Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 538,142 T→C 9.6% F151F (TTT→TTC mfd → transcription‑repair coupling factor helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730538,1420TC 9.6% 178.1 / 10.2 73F151F (TTT→TTCmfdtranscription‑repair coupling factor helicase
Reads supporting (aligned to +/- strand):  ref base T (38/28);  new base C (4/3);  total (42/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

ACTGCGTTAGCTCAAGGTAAGAAAGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAATCAGAATTGAGCTATTTGATACCGA  >  CP000730/538005‑538279
                                                                                                                                         |                                                                                                                                         
aCTGCGGTAGCTCAAGGTAAGAAAGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCtt                                                                                                                                        <  2:402992/141‑1 (MQ=255)
 cTGCGTTAGCTCAAGGTAAGAAAGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTa                                                                                                                                       >  2:109620/1‑141 (MQ=255)
   gCGTTAGCTCAAGGTAAGAAAGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAAc                                                                                                                                     <  2:99670/141‑1 (MQ=255)
     gTTAGCTCAAGGTAAGAAAGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACaa                                                                                                                                   >  1:528964/1‑141 (MQ=255)
      ttAGCTCAAGGTAAGAAAGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACaaa                                                                                                                                  <  2:486363/141‑1 (MQ=255)
      gtAGCTCAAGGTAAGAAAGGGTTATTTATCGTTCCTTTAAAGGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAAGCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACaaa                                                                                                                                  <  2:666923/140‑1 (MQ=255)
      gtAGCTCAAAGTAATAAAGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACaaa                                                                                                                                  <  1:704162/140‑1 (MQ=255)
          cTCAAGGTAAGAAAGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAACGACATTGCGTGTTGGTGAGGATATCGATGTGGACCGATTTCTTAACAAATTAg                                                                                                                              >  2:519527/1‑141 (MQ=255)
            cAAGGTAAGAAAGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGtt                                                                                                                            <  1:578402/141‑1 (MQ=255)
              aGGTAAGAAAGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTaa                                                                                                                          >  1:657395/1‑141 (MQ=255)
                  aaGAAAGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATAAGTTAatagg                                                                                                                      >  2:127713/1‑139 (MQ=255)
                  aaGAAAGGGATAGTTATCTTTCCGTTAAATGTGTTGAAAAAGGGGTGAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATg                                                                                                                      >  2:648390/1‑141 (MQ=255)
                   aGAAAGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATgg                                                                                                                     >  1:762829/1‑141 (MQ=255)
                      aaGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGt                                                                                                                  >  1:262442/1‑141 (MQ=255)
                         ggTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTCCTTAACAAATTAGTTAATATGGGGTACa                                                                                                               >  2:291647/1‑141 (MQ=255)
                         ggTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTCCTTAACAAATTAGTTAATATGGGGTACa                                                                                                               >  2:95552/1‑141 (MQ=255)
                         ggTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTCCTTAACAAATTAGTTAATATGGGGTACa                                                                                                               >  2:414871/1‑141 (MQ=255)
                             atttatCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACg                                                                                                           <  2:412579/141‑1 (MQ=255)
                             aattatCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATGGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACg                                                                                                           <  2:247207/139‑1 (MQ=255)
                              tttatCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACgg                                                                                                          >  2:142463/1‑141 (MQ=255)
                                  tCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACACATTGGTTAATATGGGGATCAAACGGGagc                                                                                                      >  2:365510/1‑139 (MQ=255)
                                     ttCCTTTAAATCCTAGTAAAAAGTGGGTAACTGCTGTTGTACGGTCGCAACACCACCAAATGACATTGCGTGTTGGTTAGTATATCGATGTAGACCAATGTCTTAACAAAGTAGTTAATATGGGGTACAAACGGGAATCCg                                                                                                   <  2:500385/141‑1 (MQ=255)
                                      tCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGt                                                                                                  <  1:466405/141‑1 (MQ=255)
                                         tttAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGAGCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGt                                                                                               >  2:598865/1‑141 (MQ=255)
                                         tttAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACAGGAATCCGTGGt                                                                                               >  2:243909/1‑141 (MQ=255)
                                                     gAAAAAGTGGTTAACTCCTGTTGAAACGTGGCAAAATCACCAAATGACATTGCGTGTCGGTGCGGATATCGATGTGGACCAATTCCTTAACAAGTTAGTTAATTTGGGGTACAAACGGGAATCCGTGGTATCGCATATTgg                                                                                   >  2:449438/1‑141 (MQ=255)
                                                          aGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTTAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAAt                                                                              >  1:282546/1‑141 (MQ=255)
                                                           gTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTTTCGCATATTGGTGAAtt                                                                             >  2:329412/1‑141 (MQ=255)
                                                           gTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATAGGATATTGGTGAAtt                                                                             >  2:741867/1‑141 (MQ=255)
                                                            tGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGCATCGCATATTGGTGAATtc                                                                            >  2:50399/1‑141 (MQ=255)
                                                                tatcTCCTGTTGAAATGTTGCAAAATCACCAAATGACATTCCGGCCAGGTGACGTTATCCGTGTGGGCCAATTTCTTATCACGCTATTTAATTCGGGGTACAAGCGGGCGTCCGTATTACCTCAGATTGGTGACGTCTCAt                                                                        <  2:306224/138‑1 (MQ=255)
                                                                  acgcccgTTAAAATGTGGCAAAATCACCAAATGACATTGCGTGTTAGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGCGGTACAAACGGGAATCCGTGGTATCGCATATTGGGGAATTCTCATTg                                                                      <  2:318951/135‑1 (MQ=255)
                                                                  accccTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGGAGCCGTCATTTCTTATTTTGGTGAATTCTCATTg                                                                      <  2:355633/138‑1 (MQ=255)
                                                                     ccTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCga                                                                   >  1:10945/1‑141 (MQ=255)
                                                                       tGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCgagg                                                                 <  2:367434/141‑1 (MQ=255)
                                                                       ggtggAAAGGTGGCAAAATCACCAATTGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCgagg                                                                 <  2:460215/137‑1 (MQ=255)
                                                                        gTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCgagga                                                                <  2:657395/141‑1 (MQ=255)
                                                                          tGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGCGAATTCTCATTGCGaggagg                                                              >  2:576529/1‑141 (MQ=255)
                                                                          tGAAATGTGGCAAAATCACCAAATGACAGTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGaggagg                                                              >  2:576472/1‑141 (MQ=255)
                                                                          tGAAATGTGGCAAAATCACAAAATGACATTGCGTGTTGGTGAGGACATCGATGTCGACCAATTTCTTAACAAAATAGTTAATATGGGGTACAAACGGGAATCCGTGGTACCGCCTATGGGTGAATTCTCATGGCGaggagg                                                              >  2:411330/1‑141 (MQ=255)
                                                                           gAATTGTGGCAAAACCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTGTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGGATCGCATATTGGTGAATTCTCATTGCGAGGAGGt                                                             <  1:149972/141‑1 (MQ=255)
                                                                                 tgGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCGCATTGCGAGGAGGTATTATc                                                       >  2:122666/1‑141 (MQ=255)
                                                                                  ggCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGGCCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGCGGAGGTATTATCg                                                      >  2:183363/1‑141 (MQ=255)
                                                                                    cAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTCCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGat                                                    <  1:291647/141‑1 (MQ=255)
                                                                                    cAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTCCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGat                                                    <  1:95552/141‑1 (MQ=255)
                                                                                    cAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTCCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGat                                                    <  1:414871/141‑1 (MQ=255)
                                                                                          cACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCttt                                              >  1:29087/1‑141 (MQ=255)
                                                                                            ctaaaTGCCAGTGCGTGTTGGTGAGGATATCGAGGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTcc                                            <  2:432011/139‑1 (MQ=255)
                                                                                            ccAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATGAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTcc                                            <  2:383203/141‑1 (MQ=255)
                                                                                             cAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCg                                           >  2:739993/1‑141 (MQ=255)
                                                                                                 tGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTaa                                       <  1:109620/141‑1 (MQ=255)
                                                                                                   aCATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATGGATATCTTTCCGCTAAtt                                     >  1:660748/1‑141 (MQ=255)
                                                                                                    cATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTg                                    >  1:617320/1‑141 (MQ=255)
                                                                                                    cATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTg                                    >  1:252082/1‑141 (MQ=255)
                                                                                                      ttGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTggg                                  <  1:200506/141‑1 (MQ=255)
                                                                                                          gtgtTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTCTAGTTTATTGTTCGCTAATTGGGGAAc                              <  2:549929/141‑1 (MQ=255)
                                                                                                              tGGTGCGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAAt                          <  2:561312/141‑1 (MQ=255)
                                                                                                              tGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAAt                          >  1:286372/1‑141 (MQ=255)
                                                                                                              tGGTGAGGATATCGATGTGGACCAAATTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAAt                          >  1:423522/1‑141 (MQ=255)
                                                                                                              tGGTGAGGATATCGAAGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGCATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGGATTATCGATATCTTTCCGCTAATTGGGGAACCAAt                          >  2:667597/1‑141 (MQ=255)
                                                                                                                 ggAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAAGTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAATCAg                       <  1:142463/140‑1 (MQ=255)
                                                                                                                    ggATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAATCAGAAt                    >  2:470078/1‑141 (MQ=255)
                                                                                                                     gATATCGTTGTGGACCAATTTCTTTAGAAATTCGTTAATATGGGGTACTAACGGGACTCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAATCAGAAtt                   <  2:742009/141‑1 (MQ=255)
                                                                                                                        atCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAATCAGAATTGAg                >  2:151431/1‑141 (MQ=255)
                                                                                                                        atCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGCGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAATCAGAATTGAg                <  2:528964/141‑1 (MQ=255)
                                                                                                                         tCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCCATCAGAATTGAGc               >  1:673552/1‑141 (MQ=255)
                                                                                                                         tCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAATCAGAATTgcgc               >  2:774238/1‑141 (MQ=255)
                                                                                                                         tCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAATCAGAATTGAGc               >  1:333757/1‑141 (MQ=255)
                                                                                                                          cGATGTGGACCAATTTCTTACCAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCCTATTGGTGAATTCTCATTGCGCGGAGGTATTAGCGATATCTTTCCGCTCATGGGGGAACCAATCGGAATTGAGCt              >  2:51798/1‑141 (MQ=255)
                                                                                                                                   ccAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAATCAGAATTGAGCTATTTGATAc     <  2:10945/141‑1 (MQ=255)
                                                                                                                                     aaTTTCTTAACTAATGAGTTAATATGGGGTACACACGGGAATCCGTGGTATCGCATATTTGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAATCAGAATTGAGCTATTTGATACCg   <  1:199111/141‑1 (MQ=255)
                                                                                                                                      aTTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAATCAGAATTGAGCTATTTGATACCGa  >  2:225904/1‑141 (MQ=255)
                                                                                                                                      aTTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAATCAGAATTGAGCTATTTGATACCGa  >  2:653414/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                         
ACTGCGTTAGCTCAAGGTAAGAAAGGGTTATTTATCGTTCCTTTAAATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTGCGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTACAAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGATATCTTTCCGCTAATTGGGGAACCAATCAGAATTGAGCTATTTGATACCGA  >  CP000730/538005‑538279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: