Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,353 G→A 49.2% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3530GA49.2% 17.8 / inf 425intergenic (+411/‑723)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (107/107);  new base A (94/114);  total (201/224)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 426 total aligned reads displayed.

TATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCATT  >  CP000730/76214‑76492
                                                                                                                                            |                                                                                                                                           
tATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGt                                                                                                                                            <  1:171322/141‑1 (MQ=32)
gaTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGt                                                                                                                                            <  2:148005/140‑1 (MQ=17)
 aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                                                                           <  1:21522/141‑1 (MQ=32)
 aTGAACTATTTCAGATATAAACAATATAACAAGGATGTTATCCCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATc                                                                                                                                           <  1:72845/141‑1 (MQ=11)
  tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATAGCGGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCt                                                                                                                                          <  1:108508/141‑1 (MQ=12)
     aCTATTTCAGATATAAACAATTTAACAAGGATGGTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                                                       <  1:87866/141‑1 (MQ=255)
      cTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCg                                                                                                                                      >  1:533516/1‑141 (MQ=17)
      cTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTGTCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCg                                                                                                                                      >  1:522799/1‑141 (MQ=255)
       tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                                                                                                                     >  2:652392/1‑141 (MQ=17)
       tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                                                                                                                     <  2:285933/141‑1 (MQ=17)
       tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                                                                                     <  2:768967/141‑1 (MQ=12)
        aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAGTATTAAGGGAACGTGGTGTAAACGTTCATCATTCCACGGTCTACCGtt                                                                                                                                    >  1:618539/1‑141 (MQ=12)
        aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                                                                                    <  2:344151/141‑1 (MQ=17)
        aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                                                                                    >  2:665849/1‑141 (MQ=17)
         tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAAAGGTCTACCGTTg                                                                                                                                   >  2:736957/1‑141 (MQ=17)
          ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTgg                                                                                                                                  <  1:656557/141‑1 (MQ=255)
           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                                                                                 <  1:511431/141‑1 (MQ=17)
           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                                                 <  2:19504/141‑1 (MQ=255)
           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                                                 >  2:227665/1‑141 (MQ=12)
            cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGt                                                                                                                                >  1:359017/1‑141 (MQ=17)
             aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAAAGGTCTACCGATgggat                                                                                                                               >  1:56998/1‑139 (MQ=12)
               atatAAACAATGTAACAAGGATGTTATCACTGGAGCCGTTGGCTACTAACGAAGATGTGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCa                                                                                                                             <  1:102567/141‑1 (MQ=11)
                 ataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTACGATCGGCAGTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                                                                                                           <  1:561495/141‑1 (MQ=11)
                 ataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                                                                                                           >  2:168143/1‑141 (MQ=17)
                 ataAACAATTTAACAAGGACGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAg                                                                                                                           <  2:724611/141‑1 (MQ=255)
                  taAACAATTTAACAAGGCTGTTAGCACTGGAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                                                                                          <  2:273135/141‑1 (MQ=11)
                    aaCAATTTAACATGGAGTTTATCACTGCAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGCTCTACCGTTGGGTTCAAGAat                                                                                                                        <  1:465976/141‑1 (MQ=11)
                    aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAat                                                                                                                        >  2:297719/1‑141 (MQ=17)
                    aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                                                                                                        <  2:685862/141‑1 (MQ=255)
                      cAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTAGGTTCAAGAatat                                                                                                                      >  1:408707/1‑141 (MQ=17)
                      cAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                                                                                      >  2:242997/1‑141 (MQ=255)
                      aaaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATACTAAGCGAATGTGGTGTAAACGTTCATACTTCACCGATCTACCGTTGGGTTCAAGAatat                                                                                                                      <  2:568827/140‑1 (MQ=11)
                       aaTTTCACAAGGATGTTATCACTGTAGCTGTTGGCTACCATCTAAGTTATGCATTGAGTTATCGTGATATATCTGACATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCGAGAATATg                                                                                                                     <  2:618051/141‑1 (MQ=11)
                       aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                                                                                                                     <  1:317419/141‑1 (MQ=12)
                        atgtAACAAGGGTGTTAGCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGc                                                                                                                    <  1:43213/138‑1 (MQ=255)
                        aTTTGACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAACGGACCGCGGGGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGc                                                                                                                    >  1:527649/1‑141 (MQ=11)
                        aTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGc                                                                                                                    >  1:416796/1‑141 (MQ=17)
                        aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGc                                                                                                                    <  1:161067/141‑1 (MQ=255)
                          ttAACAAGGATGTTATCACTTTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGccc                                                                                                                  <  2:753403/141‑1 (MQ=255)
                          ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccc                                                                                                                  >  1:297767/1‑141 (MQ=32)
                          ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCTACGATCTACCGTTGGGTTCAAGAATATGccc                                                                                                                  >  1:282828/1‑141 (MQ=255)
                           tAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcccc                                                                                                                 <  1:446756/141‑1 (MQ=32)
                            aaCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCa                                                                                                                <  1:264890/141‑1 (MQ=17)
                             aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                               >  2:503297/1‑141 (MQ=12)
                             aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                               >  1:13951/1‑141 (MQ=255)
                              cAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAt                                                                                                              >  2:123854/1‑141 (MQ=12)
                              cAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAt                                                                                                              >  1:549226/1‑141 (MQ=255)
                                aGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                                                                                            <  1:235797/141‑1 (MQ=17)
                                aGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                                                                                            >  1:601922/1‑141 (MQ=255)
                                aGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                                                                                            <  2:693323/141‑1 (MQ=12)
                                aGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTGCATCATTCAACGATCTACCGTTTGGTTCAAGAATATGCGCCAAttt                                                                                                            >  1:589721/1‑141 (MQ=11)
                                aGGATGTTATCACTGTAGCCGTTGATACGTTCCTTTGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                                                                                            >  1:373509/1‑141 (MQ=11)
                                 ggATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                                                                                                           >  2:321801/1‑141 (MQ=255)
                                  gATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCACCGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                                                                                          <  1:497901/141‑1 (MQ=18)
                                  gATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                                                                                          <  1:539404/141‑1 (MQ=18)
                                    tGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                                                        <  1:744776/141‑1 (MQ=21)
                                    tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                                                        >  2:462643/1‑141 (MQ=255)
                                    tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                                                        <  2:577098/141‑1 (MQ=255)
                                     tttATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                                                       <  1:227400/140‑1 (MQ=25)
                                     gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAGANTGCCCCAATGTTatat                                                                                                       >  1:639605/1‑141 (MQ=11)
                                     gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                                                       <  2:546588/141‑1 (MQ=255)
                                       tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGAGTTCAAGAATATGCCCCAATTTTATACCa                                                                                                     >  2:365454/1‑141 (MQ=11)
                                        aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                                                                                    >  2:488977/1‑141 (MQ=255)
                                        aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                                                                                    <  1:118743/141‑1 (MQ=255)
                                        aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGAGGTGTAAACGTTCATCATTCAACGATCTACCGTTCGGTTCAAGAATATGCCCCACTTTTATATcca                                                                                                    >  1:80251/1‑139 (MQ=11)
                                            cTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAttt                                                                                                <  1:486166/141‑1 (MQ=255)
                                             tttAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTCAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATACGCCCCAATTTTATATCAAATTTg                                                                                               <  2:553679/139‑1 (MQ=255)
                                              ttAGCCGTTGGCTACTATCTAAGATATGCATCGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                                                              <  2:293053/140‑1 (MQ=255)
                                              gTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                                                              <  2:711752/141‑1 (MQ=25)
                                              gTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                                                              <  1:261910/141‑1 (MQ=25)
                                               tcgccttgggCTAATATCTAAGAGATGCATTGAGCTATCGGGATATATCTGAAATTTTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATTTACCGTTGGGTTCCAGAATATGCCCCAATTTTATATCAAATTTGga                                                                                             <  2:216396/133‑1 (MQ=11)
                                                aGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATTATTAGACGATATACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                                                            >  2:351331/1‑141 (MQ=11)
                                                aGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTGGgaa                                                                                            >  1:326444/1‑141 (MQ=255)
                                                 gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                                                           >  1:65148/1‑141 (MQ=255)
                                                  ctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                                                                                          >  1:200787/3‑141 (MQ=25)
                                                      tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaaa                                                                                      >  1:708081/1‑141 (MQ=35)
                                                      tGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATCTTAAGAGAACGTGGTGTCAACGTTCATCATTTAAAGATCCACCGTTGGGTTCAAGAAAATGCCCTAAGTTTATATCAAATTTGTAAGaaaaa                                                                                      >  2:448713/1‑141 (MQ=11)
                                                       ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCGTAATTCAACGGTCTACAGTTGGGTTCAAGACTATGATCCACTTTTATATCATATTTGGAAGaaaaaa                                                                                     <  2:69651/141‑2 (MQ=11)
                                                       ggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                                                                                     <  2:414162/141‑1 (MQ=255)
                                                       ggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGACAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATGTCAAATTTGGAAGAAAAAg                                                                                     >  1:502709/1‑141 (MQ=11)
                                                         ctactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                   <  2:187936/141‑1 (MQ=35)
                                                          tactcTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                  >  2:535437/1‑141 (MQ=21)
                                                           actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGGGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                                                 <  2:25083/141‑1 (MQ=255)
                                                            ctaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTAAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAACATaa                                                                                >  2:264358/1‑141 (MQ=21)
                                                            ctaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                                                                >  1:517905/1‑141 (MQ=255)
                                                             ataTCTAAGATATGCATTGAGTGACCGTGATATATCCGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTCCCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                                                                <  2:403366/140‑1 (MQ=255)
                                                               aTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                                                                              <  1:693393/141‑1 (MQ=255)
                                                               aTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                                                                              >  1:155322/1‑141 (MQ=255)
                                                                 cTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa                                                                            >  1:295217/1‑140 (MQ=255)
                                                                  tAAGATATGGATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGGGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaag                                                                           <  2:524670/141‑3 (MQ=11)
                                                                   aaGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCt                                                                          <  1:280027/141‑1 (MQ=37)
                                                                      atatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagctta                                                                       <  1:183203/141‑7 (MQ=11)
                                                                       tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGACCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                                                      >  2:392034/1‑134 (MQ=0)
                                                                           cATTGAGCTATCGTGATATATCTGAAATATTAAGAGAAAGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAGAGAATaaaaaagtctattaaa                                                                  >  2:699737/1‑130 (MQ=1)
                                                                            cgtGAGTTATCATGATATATCTGAAATATTAAGGGGACGTGCTGAAAACGTTCATCAATCAACGGTCTACCGGTGGGTTCAAGCATATGCCCCAATTTTATATCAAAGTTGGAAGAAAAAGCATAAAAAGCTAATTACaaa                                                                 <  2:303972/139‑1 (MQ=14)
                                                                                    aTCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTAt                                                         <  1:347968/141‑1 (MQ=40)
                                                                                                    aaTATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACACATGGCGTATTGATGAGACGTACATc                                         <  2:512578/141‑1 (MQ=255)
                                                                                                          aaGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATa                                   >  2:331315/1‑141 (MQ=40)
                                                                                                                                caccatTCAACTGCCTGTCGTTGGGTACAAGATTATGCCCCAAGTTTATAGCAAAGGGGGGAGAAAAAGCATAAAAAGCTAATTACCAATGGCGTATTGATGTGACGTACATCAAAATAAAAGGAAAAGGGAGCTATTtat             <  2:376412/138‑1 (MQ=14)
                                                                                                                                      tCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTg       <  1:666155/141‑1 (MQ=38)
                                                                                                                                            |                                                                                                                                           
TATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCATT  >  CP000730/76214‑76492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: