Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,322 G→A 53.3% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3220GA53.3% 82.8 / inf 554intergenic (+380/‑754)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (135/121);  new base A (129/161);  total (267/287)
Fisher's exact test for biased strand distribution p-value = 5.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 564 total aligned reads displayed.

TAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATA  >  CP000730/76183‑76459
                                                                                                                                           |                                                                                                                                          
taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg                                                                                                                                           <  1:666331/141‑1 (MQ=17)
taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg                                                                                                                                           >  2:760565/1‑141 (MQ=17)
 aatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGTTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGCGa                                                                                                                                          <  2:689279/139‑1 (MQ=255)
     taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgt                                                                                                                                      <  1:193164/141‑1 (MQ=17)
     aaaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgt                                                                                                                                      <  2:187367/140‑1 (MQ=17)
         tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggtg                                                                                                                                  <  1:26817/141‑1 (MQ=17)
          ttATCAAAAAGGAGTCGTCTGTATGAACTATTTAAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGgtgt                                                                                                                                 <  2:520189/141‑1 (MQ=11)
          ttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGgtgt                                                                                                                                 <  2:113732/141‑1 (MQ=12)
           tAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCCGAAATATTAAGAGAACGTGGTGTa                                                                                                                                >  2:49291/1‑141 (MQ=12)
            aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGTTGTaa                                                                                                                               >  2:628119/1‑141 (MQ=17)
               aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGGGGTGTAAACg                                                                                                                            >  2:289581/1‑141 (MQ=12)
                   aGGAGTCTTCTGTATGAAATATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtca                                                                                                                        <  1:539750/141‑1 (MQ=17)
                   aGGAGCCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtca                                                                                                                        <  2:550972/141‑1 (MQ=255)
                    ggAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcat                                                                                                                       >  1:734863/1‑141 (MQ=17)
                    ggAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcat                                                                                                                       <  2:125041/141‑1 (MQ=17)
                       ggcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTcatcat                                                                                                                    <  2:52913/139‑1 (MQ=12)
                        tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCAtt                                                                                                                   >  1:508031/1‑141 (MQ=17)
                        tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCAtt                                                                                                                   >  1:628216/1‑141 (MQ=32)
                        tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCAATGTAGCAGTTGGATACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAAAGTTCATCAtt                                                                                                                   >  2:442654/1‑141 (MQ=11)
                          ttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCa                                                                                                                 >  2:691213/1‑141 (MQ=17)
                              gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                                                                             <  1:357963/141‑2 (MQ=12)
                                aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACgatc                                                                                                           <  2:555385/141‑4 (MQ=12)
                                aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACgatc                                                                                                           <  1:610339/141‑4 (MQ=12)
                                 tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCt                                                                                                          <  2:407285/141‑1 (MQ=17)
                                  gAACTATTTCAGATATAAACAATTTAACGAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGCAATATTAAGAGAACATGGTGTAAACGTTCATCATTAAACGATCtc                                                                                                         >  1:499611/1‑140 (MQ=11)
                                   aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                                                                                        <  1:63997/141‑1 (MQ=17)
                                   aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAGGTTCATCATTCAACGGTCTAc                                                                                                        >  2:217488/1‑141 (MQ=17)
                                    aCTATTTCAGATATAAACAATTTAACAAGGATGGTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                       <  1:87866/141‑1 (MQ=255)
                                    aCTATTTCAGATAGAACGAATTTAACAAGGATATTACCACCGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGCGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                       <  2:23429/141‑1 (MQ=11)
                                      tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                                                                                     >  2:652392/1‑141 (MQ=17)
                                      tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                                                     <  2:374560/141‑1 (MQ=255)
                                       aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                                                    >  2:665849/1‑141 (MQ=17)
                                         ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTgg                                                                                                  <  2:365344/141‑1 (MQ=12)
                                          tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAACATTAAGGGAAAGTGGTGTAAACGTCCATCATTCAACGGTCTCCAGTTggg                                                                                                 >  1:292828/1‑141 (MQ=11)
                                          tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGCTggg                                                                                                 >  2:273583/1‑141 (MQ=17)
                                          tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                 <  2:19504/141‑1 (MQ=255)
                                          tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTCAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                 <  1:516054/141‑1 (MQ=11)
                                           cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAGCGTTCATCATTCAACGATCTACCGTGGGGt                                                                                                >  2:54128/1‑141 (MQ=11)
                                           cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGt                                                                                                >  1:148466/1‑141 (MQ=255)
                                           cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGt                                                                                                >  1:260437/1‑141 (MQ=255)
                                           cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGt                                                                                                >  1:655290/1‑141 (MQ=255)
                                           cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGt                                                                                                >  1:53519/1‑141 (MQ=255)
                                            aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAAAGGTCTACCGATgggat                                                                                               >  1:56998/1‑139 (MQ=12)
                                             gATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTc                                                                                              >  1:701903/1‑141 (MQ=255)
                                              atatAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCa                                                                                             <  1:113138/141‑1 (MQ=17)
                                              atatAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCAAAGATATGCATTGCGCTACCGCGATAGATCTGAAATACTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCa                                                                                             <  1:370373/141‑1 (MQ=11)
                                              atatAAACAATGTAACAAGGATGTTATCACTGGAGCCGTTGGCTACTAACGAAGATGTGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCa                                                                                             <  1:102567/141‑1 (MQ=11)
                                                ataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                                                                           >  2:168143/1‑141 (MQ=17)
                                                ataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                                                                           <  1:276466/141‑1 (MQ=17)
                                                 taAACAAGTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                                                          <  2:415548/141‑1 (MQ=255)
                                                  aaaCAATTTAACAAGGATGTTATCACTGTTGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCCACCGTTGGGTTCAAGaa                                                                                         <  2:388638/141‑1 (MQ=11)
                                                   cactttttATCAAGGATGTTACCACTGGAGCCGTTGGCGACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGACCTACCGTTGGGTTCAAGAat                                                                                        <  1:411463/136‑1 (MQ=11)
                                                   aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                                                                        >  1:334277/1‑141 (MQ=12)
                                                    aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAata                                                                                       >  2:347968/1‑141 (MQ=32)
                                                     aaaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATACTAAGCGAATGTGGTGTAAACGTTCATACTTCACCGATCTACCGTTGGGTTCAAGAatat                                                                                      <  2:568827/140‑1 (MQ=11)
                                                      aaTTTCACAAGGATGTTATCACTGTAGCTGTTGGCTACCATCTAAGTTATGCATTGAGTTATCGTGATATATCTGACATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCGAGAATATg                                                                                     <  2:618051/141‑1 (MQ=11)
                                                       aTTTGACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAACGGACCGCGGGGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGc                                                                                    >  1:527649/1‑141 (MQ=11)
                                                       aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGc                                                                                    <  1:161067/141‑1 (MQ=255)
                                                       aTTCAACAACGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGc                                                                                    <  1:137404/141‑1 (MQ=12)
                                                          tAACAAGGATGTTATCACTGTAGTCGTTGGCTACTATCTAAGATATGCATTGAGTTATGGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGCTGGGTTCAAGAAAATGcccc                                                                                 <  1:487623/141‑1 (MQ=255)
                                                            aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                               >  2:401098/1‑141 (MQ=17)
                                                            aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                               <  1:417383/141‑1 (MQ=17)
                                                             cAAGGATTTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCTAt                                                                              >  1:282374/1‑141 (MQ=11)
                                                             cAAGGATGTTATCACTGTAGCACCAGGCGACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAt                                                                              >  2:747909/1‑141 (MQ=11)
                                                               aGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTGCATCATTCAACGATCTACCGTTTGGTTCAAGAATATGCGCCAAttt                                                                            >  1:589721/1‑141 (MQ=11)
                                                                 gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                                                          <  2:471909/141‑1 (MQ=255)
                                                                   tGTTATCACTGTAGCTGTTGGCTACGATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCTACGGTGTACCGTTGGGTTCAACACTATGCCCCAATTTtata                                                                        >  2:209518/1‑141 (MQ=11)
                                                                   tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                        <  1:344235/141‑1 (MQ=35)
                                                                   tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                        <  2:509544/141‑1 (MQ=255)
                                                                    gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                       >  2:73137/1‑141 (MQ=255)
                                                                       aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                                                    >  2:147362/1‑141 (MQ=21)
                                                                       aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                                                    >  1:434662/1‑141 (MQ=255)
                                                                       aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGAGGTGTAAACGTTCATCATTCAACGATCTACCGTTCGGTTCAAGAATATGCCCCACTTTTATATcca                                                                    >  1:80251/1‑139 (MQ=11)
                                                                        tCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                                                   >  2:712553/1‑141 (MQ=25)
                                                                         cattggtgCCGTTGGCTACTATCTAAGTTATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt                                                                  <  2:294587/134‑1 (MQ=11)
                                                                           cTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAttt                                                                >  2:708580/1‑141 (MQ=35)
                                                                             gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                              <  1:744798/141‑1 (MQ=255)
                                                                              tagccaacggCTACTATCTAAGATATGCATTGCGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTCCCGTTGGGTTCAAGAATATGCCCCAATTGTATATCAAATTTGga                                                            >  2:58475/9‑141 (MQ=11)
                                                                               agccaacggCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATAGCAAATTTGgaa                                                           >  1:23495/8‑141 (MQ=255)
                                                                                gctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                          <  2:336524/138‑1 (MQ=21)
                                                                                 gcggtGGCTAAGATCTACGATATGCATTGAGTTATCGTGATATATCTGAAACATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAACATGCCCCAATTTTATATCAAATTTGgaaga                                                         <  1:314075/137‑1 (MQ=11)
                                                                                  tgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTCTATCAAATTTGgacgaa                                                        >  1:224457/2‑141 (MQ=17)
                                                                                   gTTGGCTACTATCTAATATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaa                                                       <  1:520010/141‑1 (MQ=11)
                                                                                     tGGCTACTATCTAAGATATGCAATGAGTTATCGTGATATATCTGAAATAGTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaaa                                                     <  1:296739/141‑1 (MQ=25)
                                                                                      ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCGTAATTCAACGGTCTACAGTTGGGTTCAAGACTATGATCCACTTTTATATCATATTTGGAAGaaaaaa                                                    <  2:69651/141‑2 (MQ=11)
                                                                                        ctactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                  <  1:691213/141‑1 (MQ=35)
                                                                                          actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGGGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                <  2:25083/141‑1 (MQ=255)
                                                                                          actaTCTAAGATAGTCAGTGAGTTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                <  2:455759/141‑1 (MQ=11)
                                                                                           ctaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTAAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAACATaa                                               >  2:264358/1‑141 (MQ=21)
                                                                                           ctaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                               <  2:320063/141‑1 (MQ=255)
                                                                                            taTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                              <  1:55995/141‑1 (MQ=255)
                                                                                             aTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                                             >  1:450943/1‑141 (MQ=35)
                                                                                               ttAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa                                           <  1:716612/140‑2 (MQ=21)
                                                                                                  aGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                        <  1:208802/141‑5 (MQ=17)
                                                                                                  aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTAGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                        <  1:466392/141‑5 (MQ=11)
                                                                                                     tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                     <  1:326108/141‑8 (MQ=11)
                                                                                                          gtttgggtatctggATATATTGGAAATATTAAGGGAACGTGGTGTAAACTTTCATCATTCAACGGTCTACCGTTGGGTTCAAGATCATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACaaa                                <  2:331744/128‑1 (MQ=14)
                                                                                                                   gcttGATATACCTGAAATATCAAGGGAACTTGGTCTAAACGCTCCGCATCCACCGGCCTACCGGCTGCTTCAACAAGAAGCCCCAATCCTTTATCAAAATTAGAAGAAAAAGCATAAAAAGCTAATAACACATGGCGTAtt                       >  2:303116/4‑141 (MQ=255)
                                                                                                                          atatCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATgaga                >  2:775565/1‑141 (MQ=39)
                                                                                                                                      ttAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTGATATCAAATTTGGCCGGCCACCCCGAAAAAACCTAAAAACACATGGCGAATTGATGAGACGTACATCaaa     >  1:174642/1‑141 (MQ=1)
                                                                                                                                           |                                                                                                                                          
TAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATA  >  CP000730/76183‑76459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: