Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,946	G→A	37.7%	intergenic (‑1029/‑139)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,946	0	G	A	37.7%	32.9 / 27.1	40	intergenic (‑1029/‑139)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (13/11);  new base A (12/3);  total (25/15)
Fisher's exact test for biased strand distribution p-value = 1.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

TAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCTTTTTGTAAAATAT  >  CP000730/1929807‑1930086                                                                                                                                                 |                                                                                                                                             taataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑T‑AC‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGa                                                                                                                                             >  1:128062/1‑141 (MQ=255)   ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                                          <  1:283091/141‑1 (MQ=255)      gtagAGTCTGCTATCTCTCAGGCGTCAATTTATACGCAGATAGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCaa                                                                                                                                        <  1:31861/141‑1 (MQ=255)         gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                                                    >  1:194162/1‑137 (MQ=255)          agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑T‑TA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTAATTGGCTTAGTAAACGCaacgaat                                                                                                                                   >  1:101432/1‑136 (MQ=255)           gccGCCTATCTCTCAGGCGTCCATT‑‑GACGAAGTGAGGAGGTGCA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                                                                                                  <  1:111180/139‑7 (MQ=255)             cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaagaat                                                                                                                                >  2:74143/1‑133 (MQ=255)              gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                                                                                                                <  1:236510/141‑1 (MQ=255)              gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                                               >  2:290875/1‑132 (MQ=255)              gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                                               >  1:131133/1‑132 (MQ=255)              gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                                               >  1:131138/1‑132 (MQ=255)               ccTATCTCTCAGGCGTCAATTTAGACGCAGCGAGGCGGCGTT‑C‑AA‑T‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAGTACAAGATa                                                                                                                               >  2:219080/1‑141 (MQ=255)               ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                                                                                              >  2:140636/1‑131 (MQ=255)                cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TCAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                                             <  2:169084/141‑12 (MQ=255)                 tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaatgattaat                                                                                                                            >  2:329713/1‑129 (MQ=255)                 tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                                            >  1:119945/1‑129 (MQ=255)                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                                                           >  2:52411/1‑128 (MQ=255)                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                                                           >  1:110041/1‑128 (MQ=255)                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                                                           >  2:84230/1‑128 (MQ=255)                       tcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACat                                                                                                                       >  1:284260/1‑141 (MQ=255)                            cGTCAATTTAGACGCAGAGAGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACAtatacc                                                                                                                  >  1:758/1‑139 (MQ=255)                                               aggaggTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATcccc                                                                                               >  1:254420/1‑141 (MQ=255)                                                ggaggTTTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCt                                                                                              <  2:256696/141‑1 (MQ=255)                                                  aggTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCa                                                                                            <  2:242382/141‑1 (MQ=255)                                                     tgtA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACtt                                                                                         >  1:49376/1‑140 (MQ=255)                                                                         ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAATAATCCCCTCACTACCGCAAATAGTGAgg                                                                          <  1:211507/141‑1 (MQ=255)                                                                              ttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTCTTGTCAAATGATACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGAtt                                                                     <  1:242614/141‑1 (MQ=255)                                                                              ttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAGTAGTGGGGGGagt                                                                     >  1:32641/1‑139 (MQ=255)                                                                              ttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGAtt                                                                     <  1:51801/141‑1 (MQ=255)                                                                                          atAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAACCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTa                                                         >  2:206197/1‑141 (MQ=255)                                                                                                  aGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTa                                                 >  2:312972/1‑141 (MQ=255)                                                                                                         aGTGGCTGTGCCATTGCGTTTTTTTCATATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAACCCCCTCACTATCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATATATATTTTCGt                                          <  1:244606/141‑1 (MQ=255)                                                                                                                gtgCCATGGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTa                                   <  1:88590/141‑1 (MQ=255)                                                                                                                           tttttttCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCt                        <  1:182178/141‑1 (MQ=255)                                                                                                                             tttttCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTaa                      >  1:20786/1‑141 (MQ=255)                                                                                                                             tctttCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTGTTAATTATACTGCTaa                      <  1:164834/139‑1 (MQ=255)                                                                                                                              ttttCTTATTGGCGAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAACATCCCCTCACTACCTCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAAt                     <  2:239493/141‑1 (MQ=255)                                                                                                                                    tATTGGCTAAGTAGACGCAATACAAAATAGGTGAAATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGATGTATAAGTAAATCCTTATTTTCGTTGTCTTAATTATATTGCTAATTTTTCt               >  2:256360/1‑141 (MQ=255)                                                                                                                                        ggCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGAGGATTGGTGTATAAGAAAATACTTATTTTAGTTGTCTTAATTATACTGCTAATTTTTCttttt           >  1:225419/1‑141 (MQ=255)                                                                                                                                           tAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTGTTAATTATACTGCTATTTTTTCTTTTTTTa        >  2:263179/1‑141 (MQ=255)                                                                                                                                                 gACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCTTTTTGTAAAatat  <  1:56290/141‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                             TAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCTTTTTGTAAAATAT  >  CP000730/1929807‑1930086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑