Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | CP000730 | 1,274,207 | T→A | 100% | S204T (TCA→ACA) | USA300HOU_1186 → | CodY family transcriptional regulator |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | CP000730 | 1,274,207 | 0 | T | A | 100.0% | 54.7 / NA | 19 | S204T (TCA→ACA) | USA300HOU_1186 | CodY family transcriptional regulator |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (10/9); total (10/9) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GCATAGTGAAGTAGAAAAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATT > CP000730/1274071‑1274343 | gCATAGTGAAGTAGAAAAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCAACaaa > 2:162098/1‑141 (MQ=255) aTAGTGAAGTAGAAAAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCAACAAAAg > 2:319445/1‑141 (MQ=255) tAGTGAAGTAGAAAAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCAACAAAAGt > 1:9933/1‑141 (MQ=255) aaGTAGAAAAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCAACAAAAGTTGCAg < 2:301785/141‑1 (MQ=255) gctATTACAATGGCAATTAATTCATTATCCTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCAACAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAAtt < 1:162098/141‑1 (MQ=255) aaTGGCAATTAATTCATTATCTTATTCTGAGAAAGAAGCGATTGAACATATCTTAGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCGACAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGc < 2:245907/141‑1 (MQ=255) cAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCAACAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTAc > 1:124087/1‑141 (MQ=255) ttATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCAACAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAGTTGTAAATGCACTACGTGCATTAgga > 1:96294/1‑139 (MQ=255) aaaaaGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTAATAATCGCAACAAAAGTTGCAGACAGCCACGCCACCGCTAGATCGTTAAGTGGAAATGCACTACGTAAATTAGAAAGTGCTgggctaa > 2:234974/1‑136 (MQ=255) aaaGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCAACAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAAtt > 2:96941/1‑141 (MQ=255) agCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCAACAAAAGTTGCCGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCCCTACGTACCTCTGAATGTTTTGGTGTAGTTGAATc > 2:318625/1‑141 (MQ=255) agCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCAACAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATc < 2:108344/141‑1 (MQ=255) gCGATTACACATATCTTTGAAGAACTTGGGTGTACGGAAGGCCTATTAATCGCAACAAAAGTTGCAGATAGAGTTGGTATAACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCa < 2:35204/141‑1 (MQ=255) cGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCAACAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCAc < 1:266166/141‑1 (MQ=255) atCTTTGAAGAACTTAGCGGTACGGAAGGCCTATTAATCGCAACAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTCCGTAAATTAGAAAGTGCTGGTGTACTTGCATCACGTTCTTTAGGa > 1:247075/1‑141 (MQ=255) tGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCAACAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGaa < 2:205134/141‑1 (MQ=255) cTTGGAGGTACGGAAGGCCTATTAATCGCAACAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAgtacct < 1:69966/141‑6 (MQ=255) aTTAATCGCAACAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTTCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGa < 2:36848/141‑1 (MQ=255) cGCAACACAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAGTTGAATCACGTTCTTTAGGAATGAAAGGTACTTTGATTAAAGTTAAAAAAGAAAAAtt > 1:210457/1‑141 (MQ=255) | GCATAGTGAAGTAGAAAAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATT > CP000730/1274071‑1274343 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |