Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,909 A→T 100% M1M (TTG→ATG) † USA300HOU_0653 ← ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,9090AT100.0% 101.6 / NA 31M1M (TTG→ATG) †USA300HOU_0653ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/20);  total (11/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAG  >  CP000730/707771‑708029
                                                                                                                                          |                                                                                                                        
aCTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATAc                                                                                                                        >  1:173491/1‑141 (MQ=255)
   tGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGtt                                                                                                                     <  2:302252/141‑1 (MQ=255)
      aaaGTGAAGATTTACCAGCTACATTCGGGCAAAAGATAACAATTATCTCGCCGCGTACAGGTATCGATAAGGAAATGTTTTTAAGTACATAAAAAAAAAAAAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTtat                                                                                                                  <  2:275366/140‑1 (MQ=255)
           gAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAcctc                                                                                                             >  2:117992/1‑141 (MQ=255)
            aaGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAcctcc                                                                                                            <  1:58681/141‑1 (MQ=255)
                   aCCAGCCCCATTCGGGCCCATGATACCAATTGTTTCGCCGCGAACTGGGATCGATAAGGAAATGTTTTTAAGTACGTGCTTATTACCTAAAAAAAGATTTAAATCTTTTGTTTCTAACATACCGTTATACCTCCTAATGaa                                                                                                     <  2:11070/141‑1 (MQ=255)
                   aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGGTTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTaa                                                                                                     >  1:211808/1‑141 (MQ=255)
                   aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTGTTGTTTCTAACATACGTTTATACCTCCTAATTaa                                                                                                     <  1:322081/141‑1 (MQ=255)
                      aGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAg                                                                                                  >  2:183700/1‑141 (MQ=255)
                       gCACCATTCGGGCACAGGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTAATAACATAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGt                                                                                                 <  2:144383/141‑1 (MQ=255)
                        cACCATTCGGCACCATGATACAAATGATGTCGCCGCGTACTGGTACCGATAAAGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGtt                                                                                                <  2:272590/141‑1 (MQ=255)
                                  gCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                      <  2:211708/141‑1 (MQ=255)
                                  gCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                      <  1:302465/141‑1 (MQ=255)
                                                     tCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATaaa                                                                   >  1:323336/1‑141 (MQ=255)
                                                       gccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATCATAAACt                                                                 >  2:298344/1‑141 (MQ=255)
                                                       gccgcGTACTGGGATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACt                                                                 <  1:70365/141‑1 (MQ=255)
                                                                            ggAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                                            <  2:20739/141‑1 (MQ=255)
                                                                            ggAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATACTTATCATGTCAAg                                            <  1:323776/141‑1 (MQ=255)
                                                                              aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTACTTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                                          <  1:228426/141‑1 (MQ=255)
                                                                                 tGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                                       <  1:202956/141‑1 (MQ=255)
                                                                                    ttttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg                                    <  2:214609/141‑1 (MQ=255)
                                                                                    ttttAAGTACATGCTTATTACCGAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTAAAGTAAATTCg                                    >  2:243338/1‑141 (MQ=255)
                                                                                                      taCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAat                  <  2:333347/141‑1 (MQ=255)
                                                                                                       aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTGCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatg                 >  1:68323/1‑141 (MQ=255)
                                                                                                       aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAGGTAAATTTGTGATATTAAATAGATAatt                 >  1:198735/1‑140 (MQ=255)
                                                                                                       aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATCCGTGATATAATATAGACAatg                 >  1:34133/1‑141 (MQ=255)
                                                                                                          tAAAAACAGATTTAAATCTTCTGTTTCTAACATACGTTTATCCCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACCGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgtat              <  2:172380/141‑1 (MQ=255)
                                                                                                           aaaaaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgtatg             <  1:183700/141‑1 (MQ=255)
                                                                                                              aaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGa          <  2:173491/141‑1 (MQ=255)
                                                                                                              aaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGa          <  1:289822/141‑1 (MQ=255)
                                                                                                                      tAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGAAAAAGTATGTGAGTTTAAAg  >  2:296352/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                        
ACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAG  >  CP000730/707771‑708029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑