| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 478,502 | C→A | 100% | D342E (GAC→GAA) | USA300HOU_0457 → | NADH dehydrogenase (ubiquinone) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 478,502 | 0 | C | A | 100.0% | 83.2 / NA | 29 | D342E (GAC→GAA) | USA300HOU_0457 | NADH dehydrogenase (ubiquinone) |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/15); total (14/15) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATC > CP000730/478366‑478638 | ttCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCtt > 1:295063/1‑141 (MQ=255) cAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTAt > 1:38355/1‑141 (MQ=255) tgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCCTCTGCTAAAGAAGCTTATGGCTg > 1:292686/1‑141 (MQ=255) cATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGa > 1:123851/1‑141 (MQ=255) gtgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa < 1:207359/141‑1 (MQ=255) gtgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa < 1:29721/141‑1 (MQ=255) tttttCAGCATCTGTTATTTATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATCTTCTAAGACAAGCATCTGCTAATGTAGCTTATGGCTGGATTGTCATggg < 2:152556/138‑1 (MQ=255) atatTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATggg < 1:188157/141‑1 (MQ=255) ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCTTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACg < 1:132879/141‑1 (MQ=255) agcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTAtt > 2:101435/1‑141 (MQ=255) gATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCCTGGGACGTGTATTAGc > 2:13148/1‑141 (MQ=255) attattAATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCt < 2:144168/141‑1 (MQ=255) cGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTATATCGTGAAGCGATTTCATTTTTGAGAACAAGCAGCGGTGTAAGAATCTTATGGCGGGGTTGTCATAGGACGTGTATTAGCTATTATCCTGGCATTTCTAtt > 1:147636/1‑141 (MQ=255) ggTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGGGAAGCGATTCAATATTCCCAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAttt < 1:39598/141‑1 (MQ=255) ggTATTTTAGAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAttt < 1:309763/141‑1 (MQ=255) aTTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg < 2:268638/141‑1 (MQ=255) tttAAAGCAACATTATTTTCACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCTTGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATg < 2:219158/141‑1 (MQ=255) tAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGGGTATTAGCTCTTATCGTGGCATTTCTATTTTGGATggg > 2:243360/1‑139 (MQ=255) ttattTTTACAATCAGGGTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGa < 2:123851/141‑1 (MQ=255) tCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATTGATGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTCTTTTGGGTGAGTAGTGTCAGGAGGGCATATGag > 2:106398/1‑140 (MQ=255) ggTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGGGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgtg > 1:124527/1‑141 (MQ=255) cTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTaa > 1:86078/1‑141 (MQ=255) tCGGGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTg < 1:155440/141‑1 (MQ=255) tgttaaGTGATTCAATATGCCAAACAAAGCATCTGTTCAAGAAGTTTATGTCTTGATTGTCTTGGGACGTGTATTAGCTATGATCGTGGCATTTCTATTTTGGATGAGTAGTTACAGAAGTGCATATGAAGTTTTAAGTGc < 2:303663/137‑1 (MQ=255) gAATCGATTTAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACt < 2:128868/141‑1 (MQ=255) aGCATCTTCTAAAGAAGCTTATGGCTTGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGt < 2:295063/141‑1 (MQ=255) aGCATCTGCTAAAGAAGCTTATGGCTGGCTTGTCCTGGGACGTGAATTAGGTATTCCGCTGGCATTTCTATTTTGGATGAGAAGTGACAGAAGTGCATCTGAAGTGTTAAGTGCACTACTTCTAGGATGTTAATGACTTGt > 1:63385/1‑141 (MQ=255) gCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTa > 2:73939/1‑141 (MQ=255) aaaGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAAt > 2:31207/1‑141 (MQ=255) aagaagCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAATTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATGCTAGGATGGTCATACCTAGTAACTTGGaaac > 2:227348/1‑139 (MQ=255) | TTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATC > CP000730/478366‑478638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |