Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,474,639 C→G 100% A9129P (GCA→CCA)  ebh ← extracellular matrix binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,474,6390CG100.0% 97.9 / NA 31A9129P (GCA→CCA) ebhextracellular matrix binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (10/21);  total (10/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGCTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCATGTTCATT  >  CP000730/1474508‑1474774
                                                                                                                                   |                                                                                                                                       
tttAATTCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCa                                                                                                                                 <  1:339839/140‑1 (MQ=255)
   aaaTCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTAATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTGTTGCTTTTAAAATATTTTGAAGTGGTTGATCCAAtt                                                                                                                              >  1:70264/1‑140 (MQ=255)
     aTCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTTGATGTTATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACttgt                                                                                                                            <  1:308077/140‑1 (MQ=255)
         aTCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAACTGGTTGATCAACTTGTTCCt                                                                                                                        >  2:46472/1‑140 (MQ=255)
              gttgAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAATATATTTTGAAGTGGTTGATCGACTTGTTGCTTATTc                                                                                                                   >  1:282196/1‑140 (MQ=255)
                                  ttCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACAtt                                                                                               <  1:227437/140‑1 (MQ=255)
                                    cAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACAtttt                                                                                             >  2:62366/1‑140 (MQ=255)
                                     aaTTGGTCGATGTCAGGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGCTCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtc                                                                                            <  1:197016/140‑1 (MQ=255)
                                        tGGTCTATGTCATTTTTACCATTTTTTTTAGCAATTTCTACACTTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagc                                                                                         <  1:107557/140‑1 (MQ=255)
                                        tGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagc                                                                                         <  2:309667/140‑1 (MQ=255)
                                           ttgATGTCATGTTTACTATTTTTTTTAGCAATTGCTACACCTTCATCACCATTTAATGTTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagcatc                                                                                      <  2:166962/138‑1 (MQ=255)
                                                gtactGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCaa                                                                                 <  1:52018/136‑1 (MQ=255)
                                                    tGTTTACCATTTGTTTTAGAAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagcatcagctctccg                                                                             <  2:3491/140‑7 (MQ=255)
                                                     gTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCt                                                                            <  1:269656/140‑1 (MQ=255)
                                                     gTTTACCATTTGTTTTAGCAAGTGCTACACGATCTTCACAATATAATTTTGTTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCt                                                                            <  2:74108/140‑1 (MQ=255)
                                                     gTTTACCAATTGTTTTAGGAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCt                                                                            <  1:290473/140‑1 (MQ=255)
                                                       ttACCATTTTTTTTGGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTGAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTg                                                                          <  1:54145/140‑1 (MQ=255)
                                                         aCCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGtt                                                                        >  1:61139/1‑140 (MQ=255)
                                                                    tAGCAAGTGCTACACTTTCATCACCATTTAATTCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCAttt                                                             <  2:163183/140‑1 (MQ=255)
                                                                      gCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTg                                                           >  2:22418/1‑140 (MQ=255)
                                                                                            cATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTtgtgtg                                     <  2:313559/140‑1 (MQ=255)
                                                                                                  aTGCTTGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTGGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCt                               <  2:233981/140‑1 (MQ=255)
                                                                                                       tGTTTTGCATTTAAAATATTTTGAAGTGGTTGCTCCACTTGCTGCTTATTCGCATTTTGTTTTAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGt                          <  1:46472/140‑1 (MQ=255)
                                                                                                       tGTTTTGCATTTAAAATATTTTGAAGTGGTTGATCAACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGt                          <  1:173034/140‑1 (MQ=255)
                                                                                                         ttttGCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGGATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGttt                        <  1:157516/140‑1 (MQ=255)
                                                                                                             gCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAGTACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTc                    >  1:244375/1‑140 (MQ=255)
                                                                                                             gCATTTAAAATATTTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGccc                    >  2:16535/1‑138 (MQ=255)
                                                                                                               aTTTAAAATATTTTAAAGTGGTTAATCCACTTTTTGCTTACTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCaa                  <  1:152121/140‑1 (MQ=255)
                                                                                                                          tttGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCAtct       >  1:328039/1‑138 (MQ=255)
                                                                                                                             gAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCATGTTCa    >  1:87360/1‑140 (MQ=255)
                                                                                                                               aGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCATGTTCAtt  <  1:44832/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
TTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATATTTTGAAGTGCTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCATGTTCATT  >  CP000730/1474508‑1474774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑