BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,269	C→T	60.0%	A95T (GCA→ACA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,269	0	C	T	60.0%	171.8 / inf	558	A95T (GCA→ACA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (114/109); new base T (171/162); total (286/272)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 561 total aligned reads displayed.

GCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36130‑36409

gCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                                                                                                                             <  1:283240/141‑1 (MQ=32)

gCTACCTTCGTTGAAGAAGCGTGAGCTGTGCTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAAGATCTAATGTTTGTCCCTCTGCa                                                                                                                                             >  2:138236/1‑141 (MQ=11)

 cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                                                                                                                            <  2:159625/141‑1 (MQ=32)

   gccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                                                                                                                          <  2:154000/140‑1 (MQ=255)

   aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                                                                                                                          <  1:356824/141‑1 (MQ=17)

   aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                                                                                                                                          >  1:151574/1‑141 (MQ=32)

     cTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTGCCTCTGTATCAAt                                                                                                                                        >  1:293007/1‑141 (MQ=255)

      ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                                                                                                                                       >  1:205385/1‑141 (MQ=17)

       tCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATgg                                                                                                                                      >  2:198558/1‑141 (MQ=32)

         gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCa                                                                                                                                    <  2:335405/141‑1 (MQ=32)

         aacgAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCCATGGCa                                                                                                                                    >  2:140688/4‑141 (MQ=17)

          ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAtacaagacac                                                                                                                                   >  2:77445/1‑132 (MQ=12)

          ttGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCAc                                                                                                                                   >  1:33938/1‑141 (MQ=12)

            gAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGa                                                                                                                                 <  1:27892/141‑1 (MQ=17)

              aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAACTTGTTTACTAACGAGATTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAGCCAATTATCTAATGTATGTCCCTCTGTATCAATGGCACGtta                                                                                                                               >  1:318037/1‑141 (MQ=11)

               ggTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatat                                                                                                                              >  1:117906/1‑141 (MQ=12)

                gTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAtata                                                                                                                             >  1:250540/1‑141 (MQ=17)

                   ccTGTTCTGTTATTTCCTTTTGAGGTTTACCAAATTGTTTAATGCGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCTCTCTGCATCAATGGCACGATATAAAt                                                                                                                          <  2:9119/141‑1 (MQ=11)

                    cTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATa                                                                                                                         >  2:316577/1‑141 (MQ=255)

                      gATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCGAATGTATGTCCCTCTGCGTCAATGGCAGGATATAAAtggg                                                                                                                       >  2:357476/1‑138 (MQ=255)

                      gATCTGTAACCACCTTTTGAGGTTTACCAAATTGTTTAATTAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGc                                                                                                                       <  1:361611/141‑1 (MQ=12)

                          tttaacgaCCTTTTGAGGCTTACCAAATTGTTTAATGTGGCGTTTGATAAACGCCTATGCTGAATGATTATCTCGTTGCTTCCGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa                                                                                                                   <  1:81990/134‑1 (MQ=11)

                          tGTAACTACCTTTTTATGCTTAACAAGTTGATTAATGAGACGTTTGATAATCGTATATGCTTAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa                                                                                                                   <  2:346882/141‑1 (MQ=11)

                             aactACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGTTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGGTATAAATAGCTCCAttt                                                                                                                >  1:23501/4‑141 (MQ=11)

                             aactACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAttt                                                                                                                >  1:345915/4‑141 (MQ=255)

                               ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTc                                                                                                              <  1:59291/141‑1 (MQ=17)

                                 aCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTAGGTCCCTCTGCATCAATGGCAGGATATAATTACATCCATTATCCt                                                                                                            >  2:337895/1‑141 (MQ=11)

                                 aCCTTTTGAGGCTTACCAAATTGTTGAATGATACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCt                                                                                                            <  1:123356/141‑1 (MQ=255)

                                  tcTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtt                                                                                                           <  1:219949/140‑1 (MQ=12)

                                         aGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtttt                                                                                                    >  1:184822/1‑141 (MQ=32)

                                          ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                   <  2:259842/141‑1 (MQ=32)

                                          ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                   >  1:148457/1‑141 (MQ=32)

                                           gTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCGCCAGTTACCATTTAtttttg                                                                                                  >  2:379817/1‑139 (MQ=255)

                                            tttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCCCGATATAAATAGCTCCATTTTCCTTTTAttttttt                                                                                                 >  2:62769/1‑138 (MQ=12)

                                            tttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAt                                                                                                 <  1:114185/141‑1 (MQ=17)

                                             ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACTCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAta                                                                                                >  1:52736/1‑140 (MQ=17)

                                              tACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGt                                                                                               <  1:244954/141‑1 (MQ=32)

                                               aCCAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCTTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtata                                                                                              <  2:227319/141‑1 (MQ=255)

                                               aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTa                                                                                              <  1:100025/141‑1 (MQ=32)

                                               aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTa                                                                                              <  2:304491/141‑1 (MQ=32)

                                                  aatttGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGt                                                                                           <  1:301911/138‑1 (MQ=17)

                                                   aaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                          <  1:17668/141‑1 (MQ=17)

                                                       gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                      <  2:330871/141‑1 (MQ=32)

                                                         ttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCATCTGTATCAATGGCACGATATAAATAGCTCCATTGTCCTTTTATTTTGATATACGTCtcatca                                                                                    >  2:249131/1‑141 (MQ=255)

                                                          tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATAAACGTCCCATCaa                                                                                   >  2:51775/1‑141 (MQ=11)

                                                          tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCaa                                                                                   >  1:162580/1‑141 (MQ=17)

                                                            aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATa                                                                                 >  2:252748/1‑141 (MQ=32)

                                                             agagaTGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAc                                                                                <  2:131620/140‑1 (MQ=17)

                                                                gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCGTTTTCCTTTTATTTTGATATACGTCTCATAAATACGcc                                                                             >  2:123937/1‑141 (MQ=255)

                                                                  cGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGGTATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCAt                                                                           >  1:30948/1‑141 (MQ=17)

                                                                   gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATTCGTCTCATCAATACGCCAtt                                                                          >  1:333825/1‑141 (MQ=12)

                                                                   gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATTAATAcacactt                                                                          >  1:48387/1‑135 (MQ=11)

                                                                   gTTTGATAAACGCATATGCTGAAGGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAtt                                                                          <  2:367841/141‑1 (MQ=12)

                                                                     ttGATAATCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCATTTTg                                                                        >  1:240596/1‑141 (MQ=12)

                                                                     ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTg                                                                        <  2:118712/141‑1 (MQ=12)

                                                                     ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCCCCATCTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAtttt                                                                        >  2:294185/1‑140 (MQ=255)

                                                                     ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTg                                                                        >  2:235968/1‑141 (MQ=32)

                                                                       gATAAACGCATATGCTGAATGATTATCTCGTGGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGCTATAACTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTGGta                                                                      >  2:286318/1‑141 (MQ=11)

                                                                         tAAACGCATATGCTGAATGGTTATCTCTTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaat                                                                    <  2:231360/141‑1 (MQ=255)

                                                                          aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATTAATACGCCATTTGttata                                                                   >  1:65607/1‑141 (MQ=255)

                                                                             cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGc                                                                >  2:323203/1‑141 (MQ=25)

                                                                             cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGAACGATATAAATAGCTCAATTTTACTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGc                                                                >  2:704/1‑141 (MQ=255)

                                                                                atatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCttt                                                             <  1:215308/141‑1 (MQ=255)

                                                                                atatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCgtt                                                             >  2:262770/1‑141 (MQ=21)

                                                                                 tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttt                                                            <  2:245389/141‑1 (MQ=35)

                                                                                  aggCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTGGATATACGTCTCATCAATACGCCATTTGTAATCAGCttttt                                                           >  2:185571/3‑141 (MQ=11)

                                                                                      tGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                       <  1:297710/141‑1 (MQ=38)

                                                                                       gAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGc                                                      <  1:271443/141‑1 (MQ=255)

                                                                                       gAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATgt                                                      >  2:291843/1‑140 (MQ=38)

                                                                                           gATTATCTCGTTGCTTACGCGACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGGACGTCTCATCAATACGCCTTTTGTAATAAGCATTTTTATCCttta                                                  >  1:268716/1‑140 (MQ=11)

                                                                                           gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                  >  2:278654/1‑141 (MQ=255)

                                                                                           gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCAGTTGTACTAAGCTTTTTTATGCtttt                                                  >  1:156919/1‑141 (MQ=255)

                                                                                            aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttttt                                                 <  1:131451/141‑1 (MQ=255)

                                                                                            aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGACTTTTTAGGCttttt                                                 >  2:202942/1‑141 (MQ=11)

                                                                                              tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                               <  1:7792/141‑1 (MQ=255)

                                                                                              tATCTCGTTGCTTACGCAACCAAATATCTAATCTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTATGTCTCAGCCATACGCCATTTGTACTAAGCTTTTTTTTGCTTTttct                                               >  2:181462/1‑141 (MQ=14)

                                                                                               ttctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAACTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              <  1:258951/140‑1 (MQ=255)

                                                                                               aTCTCGTTGCTTACGCAACCAAATATCTAATGTTTGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              <  1:40187/141‑1 (MQ=255)

                                                                                                tctctgtgtttgcGCAACCATCAATCCAATGTTTGTCCTTCTGTATCAATGCCATGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATTCGCCATTTGTAATAAGCTTTTTGATGCTTTttattc                                             <  2:191876/129‑1 (MQ=11)

                                                                                                tctcGTTGCTTACGCAACCATTTGTCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                             >  1:379606/1‑141 (MQ=255)

                                                                                                   cGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaa                                          >  2:268746/1‑141 (MQ=255)

                                                                                                    gTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGTCTCATCAATTCGCCATCTATAATAATCTGTTTTATGCTTTttcttcataa                                         >  1:1055/1‑137 (MQ=11)

                                                                                                     tttcTTACTCAACCAAATATCTAATTTATGTCCCTCTGCATCAATGCCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACTTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAt                                        <  1:138688/138‑1 (MQ=14)

                                                                                                         ttACGCAACCAAATCACTAATCTATGTCCCCCAGCATCAATGGCACGATATAAATAGCTCCGTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGa                                    <  2:108705/141‑1 (MQ=14)

                                                                                                            cGCAACCAAATAACTAATGTATCTCCCTCTGTATCAATGGCACGATATAAATAGCACCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  2:164091/141‑2 (MQ=11)

                                                                                                              cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAAAATCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAATTGTAATAACCTTTTTTATGCTTTTTCTTCCAAATTTGATATaa                               >  1:167719/1‑141 (MQ=11)

                                                                                                                aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                             <  2:148457/141‑1 (MQ=37)

                                                                                                                    aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                         >  2:217596/1‑141 (MQ=255)

                                                                                                                      tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTAGGCCGGTATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                       <  1:88310/141‑1 (MQ=11)

                                                                                                                      tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                       >  2:104541/1‑141 (MQ=37)

                                                                                                                         ctcatgtatgtCCCTCGGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                    <  2:171640/138‑1 (MQ=255)

                                                                                                                          tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCCTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                   <  1:140688/141‑1 (MQ=255)

                                                                                                                          tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                   >  2:58598/1‑141 (MQ=38)

                                                                                                                           aaTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTTTTTTTCTTCCAAATTTGATATAAAATTtgggactat                  >  2:42272/1‑136 (MQ=14)

                                                                                                                             tgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATGCAAGATTGGGGCATATTc                >  1:69889/1‑141 (MQ=255)

                                                                                                                               tatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCtt              <  1:226404/141‑1 (MQ=255)

                                                                                                                                   ccccTCTTCATCCATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATATAATTTGGGCATATTCTTGAAc          <  1:196183/140‑1 (MQ=11)

                                                                                                                                    cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGAATATTCTTGAAcc         >  1:326927/1‑141 (MQ=25)

                                                                                                                                     ccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCCCATCAATACGCCATTTGTAATAAACTTTTTTATTCTTTTTCTTCCAAATTTGATGTCAATTTGGGGCATATATGTGAAccc        >  2:212657/1‑141 (MQ=11)

                                                                                                                                          gCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg   <  1:370452/141‑1 (MQ=21)

GCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36130‑36409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`