Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,843 Δ1 bp 68.6% intergenic (‑926/‑242) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8430A.68.6% 182.1 / 178.2 121intergenic (‑926/‑242)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (17/21);  new base . (46/37);  total (63/58)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 121 total aligned reads displayed.

TCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAA  >  CP000730/1929705‑1929982
                                                                                                                                          |                                                                                                                                                 
tcATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGa                                                                                                                                                 <  2:380494/141‑1 (MQ=255)
    aGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCa                                                                                                                                             >  2:252728/1‑141 (MQ=255)
                     gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GGG‑TA‑TAA‑g                                                                                                                        >  2:141597/1‑141 (MQ=255)
                        tAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑Tg                                                                                                                   <  1:374085/141‑1 (MQ=255)
                                tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTAt                                                                                                           >  1:324162/1‑141 (MQ=255)
                                  aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTCttt                                                                                                         >  1:55246/1‑141 (MQ=255)
                                    tCTAGCAGCTAGAATCTTATACCTTTTCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTc                                                                                                       <  2:153346/141‑1 (MQ=255)
                                                                aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTg                                                                           >  2:338683/1‑141 (MQ=255)
                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                         <  1:20104/127‑1 (MQ=255)
                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                         >  1:313169/15‑141 (MQ=255)
                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATAATAGCACCAGTCATCGGTGGc                                                                         >  1:22756/15‑141 (MQ=255)
                                                                  tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                        >  1:146370/14‑141 (MQ=255)
                                                                  aaGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                         <  1:41315/141‑1 (MQ=255)
                                                                  aaGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                         >  2:333846/1‑141 (MQ=255)
                                                                   cggaagtagatatgCTTGTGTATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATGATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                       >  1:248995/13‑141 (MQ=255)
                                                                   cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                       >  2:362206/13‑141 (MQ=255)
                                                                   cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CAGTAC‑T‑T‑TGCTAATCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                       <  2:191221/129‑1 (MQ=255)
                                                                   cggaagtagatatgCTTGGATATTCACCGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                       <  1:125923/129‑1 (MQ=255)
                                                                   aGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCGTAGCACCAGTCATCAGTGGCt                                                                        >  1:136971/1‑141 (MQ=255)
                                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGAGTC‑AT‑TAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                      <  1:106383/130‑1 (MQ=255)
                                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                      <  1:309083/130‑1 (MQ=255)
                                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                      <  1:121617/130‑1 (MQ=255)
                                                                    gCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                       >  1:382392/1‑141 (MQ=255)
                                                                     gaagtagatatgCTTTGATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                     <  2:39615/131‑1 (MQ=255)
                                                                      aagtaggtatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                    <  2:77730/132‑1 (MQ=255)
                                                                       agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                   <  2:284964/133‑2 (MQ=255)
                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                  <  1:286635/134‑3 (MQ=255)
                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                  <  1:245413/134‑3 (MQ=255)
                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                  >  2:353669/8‑139 (MQ=255)
                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTAGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                  >  1:341078/8‑139 (MQ=255)
                                                                         tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                 <  1:350612/135‑4 (MQ=255)
                                                                         tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATCGCACCAGTCATCAGTGGCTGTGctgt                                                                 <  2:300355/135‑4 (MQ=255)
                                                                         tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGGCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCa                                                                  <  2:332439/141‑1 (MQ=255)
                                                                          agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTAACTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCACTTTCGGTCACATCATAGCACCAGTCATAAGTGGCTGTGctgtt                                                                >  1:269373/6‑137 (MQ=255)
                                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                               <  2:29063/137‑6 (MQ=255)
                                                                             tgtgCTTTTATTTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                              <  2:26293/141‑1 (MQ=255)
                                                                             tatgCTTGTTGATTCACAGATGGTATAAAAATAGAGTCGCCTATCTCTCAGGCGTTAATTT‑‑ACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                             <  2:6360/139‑1 (MQ=255)
                                                                              atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                            >  2:47848/2‑141 (MQ=255)
                                                                              atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                            >  2:28660/2‑141 (MQ=255)
                                                                               tgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGt                                                            >  1:166975/1‑141 (MQ=255)
                                                                                gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                                                          <  2:275243/141‑3 (MQ=255)
                                                                                  ttGTATATTTGCCGATGATATAATAGTATAGTTGCCTATCGCTTAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑T‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                         <  1:107095/141‑1 (MQ=255)
                                                                                    gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                      <  1:177603/141‑7 (MQ=255)
                                                                                     tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTGTGCCATTGCGttttttt                                                      <  1:232460/141‑1 (MQ=255)
                                                                                         ttcacagagggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑A‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                 >  2:264304/12‑141 (MQ=255)
                                                                                           tGCCGATGATATAATAGTAGAGTCTCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAtt                                                <  1:336731/141‑1 (MQ=255)
                                                                                           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                               >  1:94314/5‑141 (MQ=255)
                                                                                            acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                              <  2:293779/138‑1 (MQ=255)
                                                                                             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                             >  2:47341/3‑141 (MQ=255)
                                                                                             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                             >  2:265441/3‑141 (MQ=255)
                                                                                             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                             >  1:284786/3‑141 (MQ=255)
                                                                                              agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                            >  2:57144/2‑141 (MQ=255)
                                                                                               gatggtATAGTAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GGG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                           <  2:228541/141‑2 (MQ=255)
                                                                                                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTGCCT‑TCC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                         >  1:53099/4‑138 (MQ=255)
                                                                                                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                         >  1:378026/4‑141 (MQ=255)
                                                                                                 tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTaa                                          >  2:102514/1‑141 (MQ=255)
                                                                                                 tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTaa                                          >  2:362424/1‑141 (MQ=255)
                                                                                                 gggtataATAATAGAGCCGCCTATCTCTCAGGCGCCAATT‑‑GACGAAGAGAGGAG‑GTGCGT‑TAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGCTGCGTATTATGCTTATTGGCTTAg                                         >  1:230187/4‑141 (MQ=255)
                                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCACTT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                        >  1:264537/3‑141 (MQ=255)
                                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGTG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                        <  1:374042/139‑1 (MQ=255)
                                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCCGTGGCTGTGCTGTTGCGTATTATCCTTCTTGGCTTAGt                                        >  2:56730/3‑141 (MQ=255)
                                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                        >  1:301275/3‑141 (MQ=255)
                                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                        <  2:190065/139‑1 (MQ=255)
                                                                                                  gatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                         <  2:310633/141‑1 (MQ=255)
                                                                                                   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                       >  2:115820/2‑141 (MQ=255)
                                                                                                   gtataATAATAGAGACGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                       >  1:200061/2‑141 (MQ=255)
                                                                                                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                      >  2:320387/1‑140 (MQ=255)
                                                                                                    tataATAATAGAGTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                      <  1:258578/141‑2 (MQ=255)
                                                                                                       aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                   >  1:118489/1‑141 (MQ=255)
                                                                                                       aataatAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGATGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                   >  1:30782/1‑141 (MQ=255)
                                                                                                        atatTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGGG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCTGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttactaaacgc                                  <  2:269373/141‑11 (MQ=255)
                                                                                                        ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                  <  2:66599/137‑1 (MQ=255)
                                                                                                        agaagagagCCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑G‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                  <  1:267206/136‑1 (MQ=255)
                                                                                                         taatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                 <  2:282803/138‑1 (MQ=255)
                                                                                                         taatagAGTCGCCTATCTCTCAGGCGTCAAAT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                 <  1:127303/138‑1 (MQ=255)
                                                                                                            tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                              <  2:15153/141‑3 (MQ=255)
                                                                                                             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                             >  2:64214/1‑138 (MQ=255)
                                                                                                              gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                            <  1:289772/141‑5 (MQ=255)
                                                                                                              gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGAAAACGCaacaaa                            >  2:214494/1‑137 (MQ=255)
                                                                                                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGGAAACGCaacaaat                           >  2:353305/1‑136 (MQ=255)
                                                                                                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                           <  1:52210/141‑6 (MQ=255)
                                                                                                                gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                          >  2:143415/1‑135 (MQ=255)
                                                                                                                 tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                         >  2:215102/1‑134 (MQ=255)
                                                                                                                  cGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaaaa                         <  1:103237/141‑1 (MQ=255)
                                                                                                                   gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                        <  1:258115/141‑1 (MQ=255)
                                                                                                                   gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                        >  1:182836/1‑141 (MQ=255)
                                                                                                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                       >  2:359251/1‑132 (MQ=255)
                                                                                                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                       <  2:10466/141‑10 (MQ=255)
                                                                                                                     cTATCTCTCCAGCGTCAATTTAGAGGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGCTGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAg                      >  1:280001/1‑141 (MQ=255)
                                                                                                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                     <  1:241598/141‑12 (MQ=255)
                                                                                                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                     >  1:223191/1‑130 (MQ=255)
                                                                                                                       aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGAGGCTTATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTCGACGCAAGACAAACTactt                    >  1:80999/1‑138 (MQ=255)
                                                                                                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                   >  1:144825/1‑128 (MQ=255)
                                                                                                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                   <  1:362206/141‑14 (MQ=255)
                                                                                                                            ccAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTCATGGATGAGTATACGCAATACAAAATAGGTGACat               <  1:185038/140‑1 (MQ=255)
                                                                                                                            ccAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACat               <  2:363207/140‑1 (MQ=255)
                                                                                                                             cAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGTAATACAAAATAGGTGACata              >  1:282712/1‑141 (MQ=255)
                                                                                                                                     aaTTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa      <  1:277385/141‑1 (MQ=255)
                                                                                                                                     aaTTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa      <  1:218594/141‑1 (MQ=255)
                                                                                                                                         tAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCaa  <  1:68106/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                 
TCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAA  >  CP000730/1929705‑1929982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑