Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,856,087 T→A 100% P80P (CCT→CCA USA300HOU_2698 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,856,0870TA100.0% 55.5 / NA 21P80P (CCT→CCAUSA300HOU_2698hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (9/12);  total (9/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCTAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAAT  >  CP000730/2855956‑2856221
                                                                                                                                   |                                                                                                                                      
cATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTca                                                                                                                                >  1:127177/1‑140 (MQ=255)
cATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTca                                                                                                                                <  1:247780/140‑1 (MQ=255)
      gTTGGTCCTAAAGCTATATCAAGATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAAt                                                                                                                          <  2:39770/140‑1 (MQ=255)
       ttGGTCCTAAAGCTATATTAATATTGGAACAAGCTTTATTTCCGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACCGGAGATGTGTCATGCAATCATGCGCCAAATCGTCAACAAATg                                                                                                                         <  2:84555/140‑1 (MQ=255)
        tGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGCAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTATCATGCAATAATGCGACAAAACGTCAACAAATGa                                                                                                                        >  1:245625/1‑140 (MQ=255)
           tCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAg                                                                                                                     <  2:62848/140‑1 (MQ=255)
                         cAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAgttgtt                                                                                                       >  2:96012/1‑140 (MQ=255)
                            tatTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTAca                                                                                                    <  2:236508/140‑1 (MQ=255)
                                                           aCATTTTAAAACGGAAGTTCAGTCATCATTATCTTTTAAGTTAATAGGAGATGTGTTTTGCATTCATGCGCCAAAACGTCAACACATGTTAGGTTTTATACTGGTTACAGCCGCCTTAGATATTCAataagatgtgtttt                                                                     >  2:101741/1‑128 (MQ=255)
                                                                        gAAGTTCAGTCATCATTACCTTTTAAGTTAACATGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTCACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGa                                                        <  2:186022/140‑1 (MQ=255)
                                                                           gTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTTAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatt                                                     <  2:210576/140‑1 (MQ=255)
                                                                                                 aGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGccac                               >  2:41858/1‑138 (MQ=255)
                                                                                                     aaCAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCCACAAATGATATCTTTTATAGTAGTTACAGCAGCATGATATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTg                           <  2:103255/140‑1 (MQ=255)
                                                                                                     aaCAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTg                           <  2:179748/140‑1 (MQ=255)
                                                                                                        aGGAAATGAGTCAGGGAATCATGCGCCAAAACGTCAATAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACa                        <  2:254625/140‑1 (MQ=255)
                                                                                                              tgtgTCCAGTAATCATTCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACg                  <  1:254433/140‑1 (MQ=255)
                                                                                                                     tGCATTCATGCGACAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCa           <  2:71283/140‑1 (MQ=255)
                                                                                                                     tGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCCCa           >  2:140916/1‑140 (MQ=255)
                                                                                                                        aaTCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTACATGTCCGCTTTGACATATACGGCCCTCCAat        >  1:38246/1‑140 (MQ=255)
                                                                                                                           cATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTAAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATAtt     >  1:267772/1‑140 (MQ=255)
                                                                                                                              gcgcCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAAt  >  1:227164/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                      
CATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCTAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAAT  >  CP000730/2855956‑2856221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: