| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,021,817 | C→T | 100% | V139I (GTT→ATT) | USA300HOU_1873 ← | acyl‑CoA hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,021,817 | 0 | C | T | 91.3% | 53.9 / ‑0.8 | 23 | V139I (GTT→ATT) | USA300HOU_1873 | acyl‑CoA hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (12/9); total (14/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCAC > CP000730/2021680‑2021952 | tAACAATCTGGCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAatat < 1:130570/140‑1 (MQ=255) cAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAtatcat < 2:171142/140‑1 (MQ=255) tCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTgg > 1:75121/1‑140 (MQ=255) tatTTTTTGTCTGAGATATGTTGCACTTGAGCTAAATATGCAACTTGTTGTTTACTTTCAATGCGACGTGCTTTTCTTCTTTCAACATGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATac < 2:73523/140‑1 (MQ=255) tatTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATac < 1:269189/140‑1 (MQ=255) tAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAgg < 1:31433/140‑1 (MQ=255) atGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGATACATGTTTAg > 1:277047/1‑140 (MQ=255) gAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTtca > 1:272230/1‑140 (MQ=255) gAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTtca > 2:90945/1‑140 (MQ=255) aGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATAATCTTCTGGATATACACAAGGTACTTGTTTAGGTTTGCAtttct > 2:28641/1‑137 (MQ=255) atTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTCTAGGTTTGCTTTCATCATCTAAc < 2:170151/140‑1 (MQ=255) aTTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGcaac > 1:176748/1‑140 (MQ=255) tttttACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGt < 2:32386/138‑1 (MQ=255) ttACTTTCAATGCGACGTGCTTTTCTTCGTTCACCACGTTGTGGTGCTGTATAATAGAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTGTCGGTTTGCCTTCATAATCTAACGCAACAAATGTTaa > 2:278132/1‑140 (MQ=255) tttCAATGCGGCGTCCGTTGTTGCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTaa < 1:254412/140‑1 (MQ=255) ttCAATGCGACGTGCTTTTCTTCCTTCAACTCGTTGTCTCTCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCACGTACATTTTTAGGTTTGCCTTCATCATCTAACGCATCAAATGTTAAGTAAc < 2:109939/140‑1 (MQ=255) gACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATTCACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAAGGCa > 2:216575/1‑140 (MQ=255) aCGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCac > 2:205593/1‑139 (MQ=255) gCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTAAATGTTTAGGTTTGCCTTCATCGTCTAACGCAACAAATGTTAAGTAACATAATGCAACTaa > 2:25026/1‑140 (MQ=255) gCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTAACTCTGATATTCTGGATACACCGCATGTACAAGTGAAGCAGAGCCGTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTaa > 1:129108/1‑140 (MQ=255) cGTTCATCATGTTGTGGTGCTGTATCATTAAACCTTTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAACGTTAAGTAACTTAATGCAGCTAAATCATGCtt < 1:65332/140‑1 (MQ=255) cacGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGttatta > 2:286738/1‑140 (MQ=255) tggtgCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATATTAACTAACTTAGTGCAGCTAAATCCTGCTTGTTATTAAATACAt > 1:88164/1‑140 (MQ=255) tttCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGAcc < 1:267494/140‑1 (MQ=255) tCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTCCCTCAACCAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCAc > 1:241933/1‑140 (MQ=255) | TAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCAC > CP000730/2021680‑2021952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |