Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 356,477 A→G 4.8% W75R (TGG→CGG)  brnQ1 ← LIVCS family branched chain amino acid:cation symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730356,4770AG 4.8% 242.4 / 2.7 86W75R (TGG→CGG) brnQ1LIVCS family branched chain amino acid:cation symporter
Reads supporting (aligned to +/- strand):  ref base A (23/57);  new base G (2/2);  total (26/60)
Fisher's exact test for biased strand distribution p-value = 5.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01

AATATAATTAAAGTCCATTGGTTATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACATATTTTGACCCGCTGTATGGCCA  >  CP000730/356338‑356616
                                                                                                                                           |                                                                                                                                           
aaTATAATTAAAGTCCATTGGTTATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTACAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCAt                                                                                                                                            <  1:532306/141‑1 (MQ=255)
aaTATAAATAAAGTCCATTGGTTAGGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCAt                                                                                                                                            <  1:507833/141‑1 (MQ=255)
  tatcATTAAAGTCCTTTGGTTATGCACAGGTAAAATGTGTCTTGTTCCCATTTCGTACGCGACATTTGCAGCACGTGGATTACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATgg                                                                                                                                          <  2:345384/141‑1 (MQ=255)
  tataATTAAAGTCCATTGGTTATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTGCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATgg                                                                                                                                          <  2:494225/141‑1 (MQ=255)
    taATTAAAGTCCATTGGTTATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACCCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACCTGTAAATACCGACAGCAAAAATAAACCCGAACCTTGGAt                                                                                                                                        >  2:461235/1‑141 (MQ=255)
     aaTTAAAGTCCATTGGTTATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATg                                                                                                                                       <  1:63860/141‑1 (MQ=255)
     aaTTAAAGTCCATTGGTTATGCACAGGTAAAATGTGTCTTGGACCAATTTCGTACGCGACATTTGCAGCACGTGGAATCCCGTAAAATGCGCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATg                                                                                                                                       <  1:66567/141‑1 (MQ=255)
      aTTAAAGTCCATTGGTTATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGAATGt                                                                                                                                      >  2:429259/1‑141 (MQ=255)
       ttAAAGTCCATTGGTTATGCCACGGTTTATAGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCGTGGATGta                                                                                                                                     >  1:770814/1‑141 (MQ=255)
       ttAAAGTCCATTGGTTATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCGTGGATGta                                                                                                                                     >  1:715445/1‑141 (MQ=255)
        tAAAGTCCATTGGTTATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGtat                                                                                                                                    <  1:659056/141‑1 (MQ=255)
             tCCATTGGTTATGCACAGGTAAAATGTGTCTTCTACCAACTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACACGATCCATGGATGTTTACgtt                                                                                                                               <  2:70528/141‑3 (MQ=255)
               cATTGGTTATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTg                                                                                                                             >  1:381339/1‑141 (MQ=255)
               cATTGGTTATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCAGGGATGTATACGATTg                                                                                                                             >  1:679698/1‑141 (MQ=255)
                 ttGGTTATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGcc                                                                                                                           <  1:509779/141‑1 (MQ=255)
                  tGGTTATGCGAAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGCCATGTAAATCACGACAGCAAAAATAAACTCGAACCATGGAGGTATACGATTGcgt                                                                                                                          >  1:568665/1‑139 (MQ=255)
                      tATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTacac                                                                                                                      >  1:452390/1‑141 (MQ=255)
                      tATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAAACACATATTTATACGATTGCCTacac                                                                                                                      <  1:186656/141‑1 (MQ=255)
                      tATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTAAGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTacac                                                                                                                      >  2:708715/1‑141 (MQ=255)
                        tGCACAGGTAAAAGGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACtt                                                                                                                    <  1:698748/141‑1 (MQ=255)
                            aaGGTAACATGAGTCTTGTACAAAGTTCGTACGCGACATTTCAAGTACACGGTCCAATTTAAAAGGATGGGATAGACATGTAAATTACGACAGAAAAAATCAACCCGAACCATGGATGTATACGATTGCCTACACttccga                                                                                                                >  2:318772/2‑137 (MQ=255)
                                   aaTGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTtc                                                                                                         <  1:347535/141‑1 (MQ=255)
                                       tgtatcgaaCCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTtcatca                                                                                                     <  2:462889/133‑1 (MQ=255)
                                        gtCTTGTCCCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAAGAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTcatcat                                                                                                    <  1:681848/141‑1 (MQ=255)
                                        gtCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTcatcat                                                                                                    <  2:638491/141‑1 (MQ=255)
                                          cTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTGCACTTTCAACACCTTCATCCTaa                                                                                                  >  1:466867/1‑141 (MQ=255)
                                            tGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATaaaa                                                                                                <  1:237084/141‑1 (MQ=255)
                                            ggTACCAATTGCGTACTCGAAATTTGCAGCACGTAGAATACCGTAAAATGCTCCAATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATaaaa                                                                                                <  2:62619/140‑1 (MQ=255)
                                             gTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAACATTCGCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAAt                                                                                               >  1:731980/1‑141 (MQ=255)
                                             gTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAAt                                                                                               >  1:485327/1‑141 (MQ=255)
                                               aCCAATTGCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGc                                                                                             <  1:617149/141‑1 (MQ=255)
                                                 cAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGcaa                                                                                           <  2:73332/141‑1 (MQ=255)
                                                 cAAGTTCGTATGCGACATTTGCGGCACGGGGAATACCCTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGcaa                                                                                           <  2:273131/141‑1 (MQ=255)
                                                 cAAATTCGTACGCGACAGTTGCAGCACGGGGAATACCGTAAAAGGCGCCGAATGACATGCAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGcaa                                                                                           <  1:743013/141‑1 (MQ=255)
                                                  aaTTTCGTCCGCGACAGTTGCAGCACGGGGGATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGcaac                                                                                          <  1:48127/141‑1 (MQ=255)
                                                   aTTTCGTACGCCACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGCCTATGATTGCCTACACTTTCAACACCTTCATCATAAAATGcaaca                                                                                         <  1:340440/141‑1 (MQ=255)
                                                     ttCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGcaacaac                                                                                       <  1:505791/141‑1 (MQ=255)
                                                      tCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATCAAATGcaccaaca                                                                                      >  1:174000/1‑141 (MQ=255)
                                                       cGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGcaacaacaa                                                                                     >  1:313250/1‑141 (MQ=255)
                                                       cGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGcaacaacaa                                                                                     >  1:313146/1‑141 (MQ=255)
                                                       cGTACGCGACATTTGCAGCACGTGGAATACCGGAAAATGCTCCGATAGACATGTAAATCACGAGAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACAACTTCATCATAAAATGcaacaacaa                                                                                     >  1:376195/1‑141 (MQ=255)
                                                          aCGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATaa                                                                                  >  1:110090/1‑141 (MQ=255)
                                                          aCGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTACAACAACCTCATCCTAAAATGCAACCACAATaa                                                                                  >  2:505112/1‑141 (MQ=255)
                                                             cgACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAg                                                                               <  1:141243/141‑1 (MQ=255)
                                                             cgAAATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAg                                                                               <  1:141063/141‑1 (MQ=255)
                                                              gACATTTGCAGCACTGGGAAAACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGt                                                                              <  2:731777/141‑1 (MQ=255)
                                                              gACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGt                                                                              <  1:656574/141‑1 (MQ=255)
                                                              gACATTTGCAGCACGTCGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGt                                                                              <  2:629356/141‑1 (MQ=255)
                                                                aaTTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGtaa                                                                            <  2:277166/140‑1 (MQ=255)
                                                                 aTTTGCCGCACGTCGAAATCAGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGtaat                                                                           <  1:559091/141‑1 (MQ=255)
                                                                 aTTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGtaat                                                                           <  1:436425/141‑1 (MQ=255)
                                                                 aTTAGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGtaat                                                                           <  2:677112/141‑1 (MQ=255)
                                                                  tttGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGtaata                                                                          <  2:381339/141‑1 (MQ=255)
                                                                    tGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATaaa                                                                        <  1:415739/141‑1 (MQ=255)
                                                                           cGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTCCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGt                                                                 <  1:5649/141‑1 (MQ=255)
                                                                               gAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAata                                                             <  1:416646/141‑1 (MQ=255)
                                                                               gAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAata                                                             <  1:516103/141‑1 (MQ=255)
                                                                                         aaaTGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAg                                                   <  1:499505/141‑1 (MQ=255)
                                                                                            ttcTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGc                                                <  2:310051/139‑1 (MQ=255)
                                                                                            tGCTCCGATAGACATGTCCATCATGACAGCCAAAATAAACCCGAACCATGGATATATAGAATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGc                                                >  2:536101/1‑141 (MQ=255)
                                                                                            tGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAAGGCAACAACAATAACAGTAATAAAGGGGGGGAATATGCCTGTAAgggg                                                >  1:5709/1‑140 (MQ=255)
                                                                                            tGCTCCGATAAACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGc                                                <  1:99198/141‑1 (MQ=255)
                                                                                                 cGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGc                                           <  2:477285/141‑1 (MQ=255)
                                                                                                      gACATCTAAAAGACGAAACCAACAACAATCCCCGACTCTGGATGTATACGATTGCCTACCCTTTCAACCCCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATCTGCCTGTAAGGGCAAAGCCTAGc                                      <  2:657704/141‑1 (MQ=255)
                                                                                                      aaCATGTACATCGCGACAGCAAAAATAAACCCGAACCATTGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACCACAATAACAGTAATAAACGCGAGTAATATGCCTGTACTGGCAAAGCCTAGc                                      <  2:657720/140‑1 (MQ=255)
                                                                                                       aCATGTAAATCACGACAGCAAAAATAAACCCGACCCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCa                                     <  1:263858/141‑1 (MQ=255)
                                                                                                             aactCACAACAGCAAAAATCACCCCCCACCATGGATGTATCCGATTGCCTCCCCTTGCAACACCTTCATTATAAAATGCAACAACAACAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCaa                               <  1:34892/138‑1 (MQ=255)
                                                                                                             aaaCCACGACAGGAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATAccga                               <  2:92215/141‑3 (MQ=255)
                                                                                                              aaTCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAAt                              <  1:664519/141‑1 (MQ=255)
                                                                                                               aTCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACGCTTGGAACGCCGTCAGCGTAGACTGGAACAAATAATACAGGAAGAAACTGGATTAATATGCCTGTAAGGGCAAAGCCTAGCATACCaagc                             >  2:746214/1‑139 (MQ=255)
                                                                                                                  ccGACCGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCcac                          <  1:677947/140‑1 (MQ=255)
                                                                                                                  acaaCAGCAAAAATAACCCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCcac                          <  1:236430/138‑1 (MQ=255)
                                                                                                                  aCGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCcac                          <  1:775517/141‑1 (MQ=255)
                                                                                                                     aCAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACata                       >  1:616168/1‑141 (MQ=255)
                                                                                                                        gCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACATAttt                    <  2:313250/141‑1 (MQ=255)
                                                                                                                        gCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACATAttt                    <  2:313146/141‑1 (MQ=255)
                                                                                                                         cAAACATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACATAtttt                   <  2:391989/141‑1 (MQ=255)
                                                                                                                         aaaacatcaccccGTAGCATAGATGTAAACGATTGACTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACATAtttt                   <  2:323336/131‑1 (MQ=255)
                                                                                                                                  aCCCGAACCGTGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACATATTTTGACCCGCTg          <  2:715445/141‑1 (MQ=255)
                                                                                                                                  aCCCGAACCGTGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACATATTTTGACCCGCTg          <  2:770814/141‑1 (MQ=255)
                                                                                                                                  aCCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAACATGCAACAACAATAACAGTAATAAACGGCAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACATATTTTGACCCGCTg          <  2:426610/141‑1 (MQ=255)
                                                                                                                                   cccGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACATATTTTGACCCGCTGt         >  2:200005/1‑141 (MQ=255)
                                                                                                                                   cccGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACATATTTTGACCCGCTGt         >  2:757877/1‑141 (MQ=255)
                                                                                                                                    ccGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGAAAAGCCTAGCATACCAATCCACATATTTTTACCCGCTGtt        >  1:338893/1‑140 (MQ=255)
                                                                                                                                       aaCCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACATATTTTGACCCGCTGTATgg     <  1:50845/141‑1 (MQ=255)
                                                                                                                                          cATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACATATTTTGACCCGCTGTATGGCCa  <  1:272527/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AATATAATTAAAGTCCATTGGTTATGCACAGGTAAAATGTGTCTTGTACCAATTTCGTACGCGACATTTGCAGCACGTGGAATACCGTAAAATGCTCCGATAGACATGTAAATCACGACAGCAAAAATAAACCCGAACCATGGATGTATACGATTGCCTACACTTTCAACACCTTCATCATAAAATGCAACAACAATAACAGTAATAAAGGGGAGTAATATGCCTGTAAGGGCAAAGCCTAGCATACCAATCCACATATTTTGACCCGCTGTATGGCCA  >  CP000730/356338‑356616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: