Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,516 C→A 24.7% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,5160CA24.7% 193.1 / 60.2 100Q196K (CAA→AAA) USA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (42/32);  new base A (8/16);  total (51/49)
Fisher's exact test for biased strand distribution p-value = 6.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01

Only 100 of 104 total aligned reads displayed.

CTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGA  >  CP000730/111376‑111655
                                                                                                                                            |                                                                                                                                           
cTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTc                                                                                                                                             <  1:555125/141‑1 (MQ=255)
cTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTc                                                                                                                                             <  1:555085/141‑1 (MQ=255)
atGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTc                                                                                                                                             <  2:45553/140‑1 (MQ=255)
 tGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCa                                                                                                                                            >  1:674290/1‑141 (MQ=255)
  gAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCaa                                                                                                                                           <  1:516473/141‑1 (MQ=255)
  gAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCaa                                                                                                                                           >  1:124964/1‑141 (MQ=255)
    ggAAGTTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                                                                         <  2:231038/141‑1 (MQ=255)
        attAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCaa                                                                                                                                     <  2:298675/140‑1 (MQ=255)
          tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                                                                   <  1:110651/141‑1 (MQ=255)
           aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                                                                  >  2:248895/1‑141 (MQ=255)
           aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCATTATAAATTa                                                                                                                                  >  1:679297/1‑141 (MQ=255)
            aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATAAATTaa                                                                                                                                 >  2:443517/1‑141 (MQ=255)
             agaagaAAACATAAAAAAAGAAATAGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAAc                                                                                                                                <  1:232870/141‑1 (MQ=255)
                 gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAat                                                                                                                            <  1:583594/141‑1 (MQ=255)
                 gaaAACATAAAAAAACAAATCGAAAACTTTAAGTTTTCTGCGCTATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATTTAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAat                                                                                                                            <  1:545871/141‑1 (MQ=255)
                  aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatg                                                                                                                           <  1:317848/141‑1 (MQ=255)
                     aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgatg                                                                                                                        >  1:633555/1‑141 (MQ=255)
                      aaTAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAAtgatga                                                                                                                       <  1:77016/140‑1 (MQ=255)
                       aTAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAAATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGACGAGTTATTCGACTCAATATAAATTAACTAATtatgat                                                                                                                      >  2:413539/1‑141 (MQ=17)
                        tAAAAAAAGAAATCGAAAACTTTGAGTTTTTTTCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAtt                                                                                                                     >  2:555620/1‑141 (MQ=255)
                        tAAAAAAAGAAATCGAAAACTTTAAGCTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAtt                                                                                                                     >  2:714434/1‑141 (MQ=255)
                        gaaaaaaaGAAATCGAAAACTTTAAGGTTTGTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGCGTTATTCGGCTAAATATCAATTAACTAATGATGAtt                                                                                                                     <  2:252714/140‑1 (MQ=17)
                        aaaaaaaaGAAATCGAAAACGTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAtt                                                                                                                     <  1:47434/140‑1 (MQ=255)
                         aaaaataGAAAACTAAAACTTTAAGTTTTTTGAGCAATATAGCAAATTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATta                                                                                                                    <  2:576694/141‑1 (MQ=18)
                          aaagaataaagggAAACCTTTAAGTGTTTTGCGCAATGTAGGAATTTTAAAGATTTAAGGAATTATAAAGATGGAGATATATCATATAATCCAGAGGGGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                                   <  2:296962/129‑1 (MQ=18)
                          aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                                   >  1:663204/1‑141 (MQ=255)
                           aaaaaaaaATCAAAAACATTAAATTTTTTGCGCAACATAGCAATTTTAAATATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtata                                                                                                                  <  2:529912/141‑1 (MQ=18)
                           aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtata                                                                                                                  <  1:241681/141‑1 (MQ=255)
                                 aaaTCGAAAACTTTAAGTTTTTTGCGGAATATAGCAATTTTAAAGATTTAATGAAGTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCCGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                            <  1:594665/141‑1 (MQ=255)
                                 aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                            <  2:124964/141‑1 (MQ=255)
                                   aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATGATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                          >  1:517172/1‑141 (MQ=255)
                                    tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAc                                                                                                         >  1:712610/1‑141 (MQ=255)
                                     cGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATAAATTAACTACTGAAGATTATAATGTAAAACa                                                                                                        >  2:573373/1‑141 (MQ=18)
                                           cTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                                                                  <  2:100388/141‑1 (MQ=255)
                                             ttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTGAGTACCAATTAACTAATGATGAATATAATGTAAGACAATTaagga                                                                                                >  1:265463/1‑137 (MQ=18)
                                             ttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTCCgaa                                                                                                >  2:681113/1‑139 (MQ=255)
                                             ttAAGTTTTTTGCGCAAGATAGCAAGTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTa                                                                                                <  1:314339/141‑1 (MQ=255)
                                              tAAGTTTTTTGCGTAATATAGCAATTTTAAAGATTTAATGAAGTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                                                               <  1:601283/141‑1 (MQ=17)
                                              tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                               >  1:685925/1‑141 (MQ=255)
                                              tAAATTTTTGGCGGAATACTGCCACTTTAAATATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                               <  2:158597/141‑1 (MQ=17)
                                                aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaaaa                                                                                             >  1:650130/1‑141 (MQ=255)
                                                        gcgcAATATAGCAATCTTAAAGATTTCATGAATTCTAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTATCTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGat                                                                                     <  1:470277/141‑2 (MQ=14)
                                                          gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGataa                                                                                   >  2:600571/1‑138 (MQ=255)
                                                          gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                                   <  1:300482/141‑1 (MQ=255)
                                                           cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                                  <  1:106475/141‑1 (MQ=255)
                                                            aaTATAGTAATTTTAAACATTTAATTAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAc                                                                                 <  1:680374/141‑1 (MQ=11)
                                                              tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccg                                                                               <  2:243920/141‑2 (MQ=14)
                                                               ataGCAATTTTAAAGATTTAATGAAGTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTACTGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccga                                                                              <  2:427265/141‑3 (MQ=14)
                                                                 aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacg                                                                            <  1:397643/141‑5 (MQ=11)
                                                                 aGCAATTTTAAAGATTTAACGAGCTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGGAAAATATATGATATACcgacg                                                                            <  1:397730/141‑2 (MQ=11)
                                                                  gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATGTAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                           >  2:649307/1‑141 (MQ=255)
                                                                  gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacga                                                                           <  1:518243/141‑6 (MQ=11)
                                                                       tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaaa                                                                      <  1:630484/141‑1 (MQ=255)
                                                                        ttAAAGATTTAATGCATTATAAAGATGGATATATATCGTATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAGAAAATTACGCACAAGATAGGAtataaagacgattaaag                                                                     >  2:579634/1‑128 (MQ=11)
                                                                          aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAATGATATGACATACCAACAAATAAAgcg                                                                   >  1:468467/1‑141 (MQ=255)
                                                                           aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAGTGTAAAAAAATTACGTAAAAGAAATGACATACCAACAAAGAAAgcgc                                                                  >  1:152114/1‑141 (MQ=255)
                                                                           aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcgc                                                                  >  1:707411/1‑141 (MQ=255)
                                                                              aTTTATTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                               >  2:69763/1‑141 (MQ=255)
                                                                                  aaTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCt                                                           <  1:637238/141‑1 (MQ=255)
                                                                                    tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGGGCCGAAGCtgt                                                         >  2:720723/1‑141 (MQ=255)
                                                                                     gAATTAAAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgtt                                                        <  2:752493/141‑1 (MQ=255)
                                                                                       aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGGGCCGAAGCtgttgt                                                      >  2:468676/1‑141 (MQ=255)
                                                                                         tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGttgttg                                                    <  2:477261/141‑1 (MQ=255)
                                                                                         tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGttgttg                                                    <  2:360616/141‑1 (MQ=255)
                                                                                          ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                                   >  1:298958/1‑141 (MQ=255)
                                                                                          ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                                   >  1:298736/1‑141 (MQ=255)
                                                                                             aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAg                                                >  1:706851/1‑141 (MQ=255)
                                                                                              aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                               >  1:268135/1‑141 (MQ=255)
                                                                                               gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGt                                              <  1:256941/141‑1 (MQ=255)
                                                                                               gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGAAATAACCACAAAACAAGCGCAGAAGCtttcggtcaaatgt                                              <  2:650130/141‑14 (MQ=17)
                                                                                                         tatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAtt                                    <  2:305257/141‑1 (MQ=255)
                                                                                                         tatCATATAATCCAGAGGTGCCGAGCTATTCAGCTCAATATCAAGTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAtt                                    >  1:424130/1‑141 (MQ=255)
                                                                                                           tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATCCCAACAAATAGAGCGCCGAAGCTGTTGTTACAAGGTAAAGGGAATTTa                                  >  2:436644/1‑141 (MQ=17)
                                                                                                           tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTa                                  >  1:588603/1‑141 (MQ=255)
                                                                                                           tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATACTGAGTATAATGTAAAACACTTACGTAAAAGAGCCGAGAAACCAACAAAAAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTa                                  >  2:163044/1‑141 (MQ=17)
                                                                                                            cATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGTTTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTCAAAGGTACAGGGAATTTaa                                 <  1:433807/141‑1 (MQ=255)
                                                                                                             atatAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGATGTGGCGGAAAGGTACAGGGAATTTaaa                                <  2:550642/141‑1 (MQ=255)
                                                                                                                 aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                            <  1:555620/141‑1 (MQ=255)
                                                                                                                   tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACGGGGAATTTAAAAGGTtc                          >  1:301285/1‑141 (MQ=255)
                                                                                                                    ccAGGGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAAAGCAAATTAAGTACGAAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                         >  1:529337/1‑141 (MQ=14)
                                                                                                                    ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGGGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                         >  1:701163/1‑141 (MQ=255)
                                                                                                                       gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatca                      <  1:377412/141‑1 (MQ=255)
                                                                                                                       gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGGTGAAAGGTACAGGGAATTTAAAAGGTtcataa                      >  2:26347/1‑139 (MQ=255)
                                                                                                                         ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGt                    >  1:446192/1‑141 (MQ=255)
                                                                                                                         ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGt                    >  2:238474/1‑141 (MQ=255)
                                                                                                                          gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTGTAATGTAAAACACTTACGTAAAAGATATGACATACAAAAAAATAAAGCGCCGAAGATATTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                   >  2:710040/1‑141 (MQ=14)
                                                                                                                            gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCAGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCAACAGTTgg                 >  2:77495/1‑141 (MQ=255)
                                                                                                                              cGAGTTGTTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGGACAGGGAATTTAAAAGGTTCATCAGTTGGat               >  2:427267/1‑141 (MQ=255)
                                                                                                                              cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATAAGTTGGat               >  1:169825/1‑141 (MQ=255)
                                                                                                                              cGAGTTATTCAGCTCAATATCAAATAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCTCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat               <  2:187980/141‑1 (MQ=255)
                                                                                                                               aaGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCGGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata              <  1:261630/140‑1 (MQ=255)
                                                                                                                                aGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTCTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAgaggggtag             >  2:640303/1‑133 (MQ=255)
                                                                                                                                 gTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGAtata            >  1:734639/1‑141 (MQ=255)
                                                                                                                                  ttattaAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAATGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaa           <  2:640769/141‑1 (MQ=255)
                                                                                                                                  ttATTCAGCTTAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaa           <  1:248423/141‑1 (MQ=255)
                                                                                                                                      tttctgcAATATCAATTAACTAATGATGATTATAATGTAAATCAATGACGTCATAGTTCAGACATGTCTACAATTAAAGCGCCTGAGCTTTTGTTGAATGGTACAGGGAATTTAAAAGGTTCATCAGTTCGATATaaaaaa       <  2:379917/135‑1 (MQ=9)
                                                                                                                                      tCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaa       >  2:181290/1‑141 (MQ=255)
                                                                                                                                        aGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAt     >  1:659343/1‑141 (MQ=255)
                                                                                                                                         gCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGGACAAGGAATTTAAAAGGTTCATCAGTTGGATATAAAAACAtt    >  1:775262/1‑141 (MQ=255)
                                                                                                                                           tCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGa  >  1:426020/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGA  >  CP000730/111376‑111655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: