Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,211,670 C→A 5.3% intergenic (‑396/+150) USA300HOU_2086 ← / ← USA300HOU_2087 hypothetical protein/hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,211,6700CA 5.3% 268.4 / 2.2 83intergenic (‑396/+150)USA300HOU_2086/USA300HOU_2087hypothetical protein/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (31/47);  new base A (3/2);  total (34/49)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

TATACTGCTACACTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACATTATTTGGA  >  CP000730/2211533‑2211809
                                                                                                                                         |                                                                                                                                            
tataCTGCTACACTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACAtt                                                                                                                                           <  1:390591/141‑1 (MQ=255)
 ataCTGCTACACTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACAttt                                                                                                                                          <  2:435955/141‑1 (MQ=255)
 agaattcTACCCTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACAttt                                                                                                                                          <  2:154176/135‑1 (MQ=255)
     tGCTACACTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGaaa                                                                                                                                      >  2:340558/1‑141 (MQ=255)
     tGCTACACTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCAAATTTGaaa                                                                                                                                      >  2:106393/1‑141 (MQ=255)
        gacacTGATGAATCATTGTGGCAAGAGGACATTGCGATGGATCATGAGAACTCTGTTATTAACCACTTTTTACAGTCTGAAAACTCTTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAa‑tca                                                                                                                                  <  2:13599/140‑1 (MQ=255)
         acacTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAa‑tcat                                                                                                                                 >  1:562330/1‑141 (MQ=255)
          cacTGATGAATCATTGTGTAAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAAGAGCAGTAGGATGACTTTCACCTTTGAAa‑tcatt                                                                                                                                >  2:434310/1‑140 (MQ=255)
           acTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCt                                                                                                                               >  1:753292/1‑141 (MQ=255)
              gatgaATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTAc                                                                                                                            <  1:430444/141‑1 (MQ=255)
                taaaTCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTCCAAACTCATTAATATTACTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTActg                                                                                                                          <  2:705470/139‑1 (MQ=255)
                  aaTCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTactact                                                                                                                        >  2:24098/1‑141 (MQ=255)
                      agtgtgTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGttt                                                                                                                    <  1:423786/139‑1 (MQ=255)
                      aTTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCGAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGttt                                                                                                                    >  1:433943/1‑141 (MQ=255)
                       ttGTGTCAAGATGATATTGCGATGAAGAATGACACCTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTc                                                                                                                   <  2:638860/141‑1 (MQ=255)
                             cAAGATGACATTGCGATGAAGAATGACAACTCTNTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTa                                                                                                             <  1:131599/141‑1 (MQ=255)
                             cAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATAGTATTTCAAATAAAGACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGGTTCTATTTa                                                                                                             >  2:222355/1‑141 (MQ=255)
                             cAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACGCAAAACGCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCGGTTTCTAtttt                                                                                                             >  1:146997/1‑140 (MQ=255)
                              aaGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCAACTTCTCGCTGTTTATATTTAt                                                                                                            >  2:339466/1‑141 (MQ=255)
                              aaGATGACATTGCGATGAAAAATGACAATTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTAt                                                                                                            <  2:87813/141‑1 (MQ=255)
                               agaagaCATCGCGATGAAGAATGACAACTCTGTTATTAACCCCTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATc                                                                                                           <  2:738851/141‑1 (MQ=255)
                               aGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATc                                                                                                           <  2:516536/141‑1 (MQ=255)
                               aGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATc                                                                                                           <  2:774186/141‑1 (MQ=255)
                               aGATGACATTGCGATGAAGAATGACAACTCTGTAATTAACCACTTTTTACATACTGAAAACTCGTTAGTATTATTTCAAATACAGACAGCAGTAGGAAGACATTCACCTTTGAAA‑TCATCTTACGGCTGCTTCTTGTTATc                                                                                                           >  1:1052/1‑141 (MQ=255)
                                    aCATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGAAGTAGGATGACTTTCACATTTTAAA‑TAATATTACTGCTATTTCTATTTCTCACtta                                                                                                      >  2:660796/1‑141 (MQ=255)
                                     cATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACatat                                                                                                     >  1:613338/1‑141 (MQ=255)
                                       ttGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTg                                                                                                   <  2:557216/141‑1 (MQ=255)
                                       atGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTg                                                                                                   <  2:612192/140‑1 (MQ=255)
                                        tGCTATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGt                                                                                                  <  1:516841/141‑1 (MQ=255)
                                        tGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGt                                                                                                  <  1:144985/141‑1 (MQ=255)
                                          cGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAGAAACAGCAGTAAGATGA‑TTTCAAATGTGAAAGTCATCCTACTGCTGTTTTTAtttgaaataatattaac                                                                                                <  1:647501/141‑15 (MQ=14)
                                              gaagaaTGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAAtg                                                                                            <  1:318465/141‑1 (MQ=255)
                                                    tGACACCTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGacac                                                                                      <  2:728445/141‑1 (MQ=255)
                                                    tGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGacac                                                                                      <  1:168166/141‑1 (MQ=255)
                                                     taCAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACt                                                                                     <  1:227446/140‑1 (MQ=255)
                                                         aCTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGt                                                                                 <  1:722829/141‑1 (MQ=255)
                                                           tctGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCCAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTGTATTTTGCACATATTGTATAATGTGACACTAAGttt                                                                               >  1:369704/1‑141 (MQ=255)
                                                            ttGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTc                                                                              <  2:653000/140‑1 (MQ=255)
                                                            ctGTTATTAACCATTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTc                                                                              <  1:207891/141‑1 (MQ=255)
                                                             tGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTATGTGTCg                                                                             <  2:91388/141‑1 (MQ=255)
                                                             tGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCg                                                                             <  1:522247/141‑1 (MQ=255)
                                                             tGTTATTAACCACTTTTTACATACTGAAAACCCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCg                                                                             <  2:120308/141‑1 (MQ=255)
                                                              gTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCCGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGc                                                                            >  2:396890/1‑141 (MQ=255)
                                                                 attaACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTAt                                                                         >  2:770971/1‑141 (MQ=255)
                                                                  ttaACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCttca                                                                        <  1:440446/141‑4 (MQ=255)
                                                                      ccACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAg                                                                    >  2:599334/1‑141 (MQ=255)
                                                                       cACCTTCTACATACTGAAGACTCGTTAATATTAGTTCAGATGAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTAGTGCTGTCCCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGc                                                                   <  2:776368/141‑1 (MQ=255)
                                                                        aCTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCg                                                                  <  2:209509/141‑1 (MQ=255)
                                                                          tttttACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGaa                                                                >  1:11718/1‑141 (MQ=255)
                                                                           ttttACATACTGAAAACTCGTTAATATTAGTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTGTGATGTGACACTAAGTTTCGCTATTGAAGCGaca                                                               >  2:400278/1‑139 (MQ=255)
                                                                             ttACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGaaaaa                                                             <  1:4874/141‑1 (MQ=255)
                                                                               aCATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAaata                                                           <  2:238523/141‑1 (MQ=255)
                                                                                cctACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAaataa                                                          <  2:606118/139‑1 (MQ=255)
                                                                                 aTACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAaataat                                                         >  2:499365/1‑141 (MQ=255)
                                                                                   aCTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAAtgt                                                       <  1:106393/141‑1 (MQ=255)
                                                                                   aCTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTAGCTATTGAAGCGAAAAATAAtgt                                                       >  1:54226/1‑141 (MQ=255)
                                                                                      taaaaCTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGACGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTgcg                                                    <  2:654277/140‑1 (MQ=255)
                                                                                       aaaaCTCGTTAAGATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACACTTTAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTACAATGTATAACTAAGTTTCGCTATTGAAGCGAAAGAAAATGTccgc                                                   >  2:320002/1‑141 (MQ=255)
                                                                                        aaaCTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGcc                                                  >  1:300541/1‑141 (MQ=255)
                                                                                         aaCTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAACCGAAAACTAATgcccgccc                                                 >  2:228327/1‑141 (MQ=255)
                                                                                           ccccttAATATTATTTCAACTAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTACTAATCCCACTTTGTATAATGTGACCCTAAGGTTCGCTATTGAAGCGAAAAATAATGTGCGCCCta                                               <  1:182937/137‑1 (MQ=255)
                                                                                             cGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATATTGTGCGCCCtata                                             >  1:232531/1‑141 (MQ=255)
                                                                                             cGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCtata                                             <  2:221512/141‑1 (MQ=255)
                                                                                             cGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCtata                                             >  2:569410/1‑141 (MQ=255)
                                                                                                tAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAg                                          >  2:270334/1‑141 (MQ=255)
                                                                                                 aaTATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGt                                         <  2:102602/141‑1 (MQ=255)
                                                                                                  atatTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACTCTAAGTTGCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGtt                                        <  1:340558/141‑1 (MQ=255)
                                                                                                                 gaaaCAGCAGTAAGATGA‑TTTCAAATGTGAAAGTCATCCTACTGCTGTTTTTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTGTCTTCAAc                         >  1:11714/2‑141 (MQ=21)
                                                                                                                 aaaaCAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTACAATTATCTTCAAc                         >  2:644092/1‑141 (MQ=255)
                                                                                                                  aaaCAGCAGTATGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACt                        <  1:382558/141‑1 (MQ=255)
                                                                                                                  aaaCAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTGTTGAAGCGAAAAATAATTTGCGCCCTATAAGGGTAAAATTGTATTCAACt                        >  1:77249/1‑141 (MQ=255)
                                                                                                                  aaaCAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACt                        >  1:481288/1‑141 (MQ=255)
                                                                                                                     cagcggTAGGTTGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACATTAAGTTGCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACtttt                     <  2:156833/141‑1 (MQ=255)
                                                                                                                      agcagTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTa                    <  2:611126/141‑1 (MQ=255)
                                                                                                                       gcagTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAg                   <  1:632512/141‑1 (MQ=255)
                                                                                                                         agTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAggg                 <  1:120913/141‑1 (MQ=255)
                                                                                                                          gTAGGATGCTTTTCAACTTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGt                <  1:96901/141‑1 (MQ=255)
                                                                                                                          gTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAgggg                >  2:542297/1‑140 (MQ=255)
                                                                                                                          gTAAGATGA‑TTTCAAATGTGAAAGTCATCCTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGATAAATAATGTGCGCCCTATAAAGTTTAAGTTATCTTCAACTTTTATGGt                >  2:400194/1‑141 (MQ=25)
                                                                                                                              gATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCTCTATTGATTCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGcac            <  1:529416/141‑1 (MQ=255)
                                                                                                                              gATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGcac            <  2:42437/141‑1 (MQ=255)
                                                                                                                                    tttCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTCGGGTGCTCattatt      <  2:771137/141‑1 (MQ=255)
                                                                                                                                      tCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATCTTGTATCATCTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCGATAAAGTTATAATTGACTTCAACTGTTAGGGTGCACATTATTTg    >  1:258904/1‑141 (MQ=255)
                                                                                                                                        acaTTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACAGCTTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACATTATTTGGa  <  2:23920/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
TATACTGCTACACTGATGAATCATTGTGTCAAGATGACATTGCGATGAAGAATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCTATAAAGTTAAAATTATCTTCAACTTTTAGGGTGCACATTATTTGGA  >  CP000730/2211533‑2211809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑