Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,879 A→T 100% L11I (TTA→ATA)  USA300HOU_0653 ← ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,8790AT100.0% 166.7 / NA 54L11I (TTA→ATA) USA300HOU_0653ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (24/30);  total (24/30)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAAT  >  CP000730/707745‑708013
                                                                                                                                      |                                                                                                                                      
ggTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTTTAAACAg                                                                                                                                  >  1:93047/1‑141 (MQ=255)
        tAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTCGTATCGATAAGGGAATGTTTTTACGTACATGCTATTAACCAATAACCAGATTTaaca                                                                                                                          >  2:404135/1‑139 (MQ=255)
          aaTTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCTGCGTACTGGTATCGATACGGAAATGCCTTTAAGTACGTGCTTATTACCTATAAACAGATATAAATCt                                                                                                                        >  2:90162/1‑141 (MQ=255)
                    ttagcgtcTTGATAAGGTAAGATTTACCCGCACCATTCGGGCCCATCATACCAATTTGTTCGCCGCGTACTGGTATCGATAAGCAAATGTTTTTAAGTACATGCTTATTACCTATACACAGATTTAAATCTTTTGTTTCTa                                                                                                              <  1:86719/134‑1 (MQ=255)
                       aaGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTTTAACa                                                                                                           >  2:639366/1‑141 (MQ=255)
                          acctGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATCATTTCGCCGCGCACTGGTATCGATAAGGAAATGTCTTTAAGCACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAAc                                                                                                        <  1:144279/138‑1 (MQ=255)
                           cTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACg                                                                                                       <  1:659720/141‑1 (MQ=255)
                            ttGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTCTCGATAAGGAAGTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGt                                                                                                      >  2:601074/1‑141 (MQ=255)
                             tGATAAGGGAAGATTTACCAGCCCCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGtt                                                                                                     <  2:635847/141‑1 (MQ=255)
                                tAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAAGAGATTTAAATCTTTTGTTTCTAACAAAGGTTtat                                                                                                  >  2:254571/1‑141 (MQ=255)
                                     gAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATAcctc                                                                                             <  2:557478/141‑1 (MQ=255)
                                      aaGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTTCTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATAcctcc                                                                                            <  1:494441/141‑1 (MQ=255)
                                      aaGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATAcctcc                                                                                            <  1:205081/141‑1 (MQ=255)
                                             aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTaa                                                                                     >  1:661554/1‑141 (MQ=255)
                                               cAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGAGTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTaaaa                                                                                   >  2:392855/1‑141 (MQ=255)
                                                aGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCtaaataaacg                                                                                  >  2:307694/1‑139 (MQ=255)
                                                  cagcATTCGGGCCCATGAAACCAATGATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGtt                                                                                <  2:631983/138‑1 (MQ=255)
                                                  cACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGtt                                                                                <  2:32718/141‑1 (MQ=255)
                                                      aTTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAgg                                                                            >  1:126249/1‑141 (MQ=255)
                                                        tCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTAGCGAATAGGAAATGTTTTTCAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACATATATGACTCCTAGATAAACGTTTAgtgt                                                                          >  2:703171/1‑138 (MQ=255)
                                                            gCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                      <  2:91190/141‑1 (MQ=255)
                                                             cccATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCt                                                                     <  2:715997/141‑1 (MQ=255)
                                                               cATGATTCCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaa                                                                   <  2:98752/141‑1 (MQ=255)
                                                               cATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaa                                                                   <  2:183069/141‑1 (MQ=255)
                                                               cATGATAACAATTATTTCGCCGCGTTCTGGTCTCGATAAGCAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaa                                                                   <  2:151225/141‑1 (MQ=255)
                                                                  taTACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaatta                                                                <  2:742061/140‑1 (MQ=255)
                                                                      cgaaTTATTTCGCCGCGTACTGGGAGCGATAAGGAAATGTTTTTAAGTACATGCTTATTGCCTGTAAACAGATGTAAATCTTTTGTTTCTAACAAACGTTTATACATGCTAAGTAAAAGTTTAGGGTAACCtaagtggttg                                                            >  2:414251/3‑134 (MQ=255)
                                                                      ccAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAACCGTTTCTACCTCCTAATTAAAAGTTTAGGCTAACCTaattaattt                                                            >  2:104032/1‑140 (MQ=255)
                                                                                cgccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAAc                                                  <  1:197376/141‑1 (MQ=255)
                                                                                cgccgcGTACTGGTATCGATAAGGAAATGTATTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAAc                                                  >  1:345058/1‑141 (MQ=255)
                                                                                 gccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACt                                                 >  1:239754/1‑141 (MQ=255)
                                                                                 gccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACt                                                 >  1:719241/1‑141 (MQ=255)
                                                                                 gccgcGTACGTGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACt                                                 <  2:120719/141‑1 (MQ=255)
                                                                                           ggTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAttt                                       >  1:14164/1‑141 (MQ=255)
                                                                                           ggTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAttt                                       >  1:134999/1‑141 (MQ=255)
                                                                                                    aaGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCa                              <  2:702231/141‑1 (MQ=255)
                                                                                                    aaGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCa                              <  2:549056/141‑1 (MQ=255)
                                                                                                     atgAAATTTTTTTAAGTACTTGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCaa                             <  2:407177/139‑1 (MQ=255)
                                                                                                      ggAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                            <  1:279691/141‑1 (MQ=255)
                                                                                                        aaaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                          <  1:275935/141‑1 (MQ=255)
                                                                                                            ttttttAATTACATACTTATTACCTATAAACAGATTTAAATCTTTTGTTTATAACAAACGTTTATACCTCCTAATAAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAtt                      <  2:84778/140‑1 (MQ=255)
                                                                                                             tttttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTc                     >  1:626974/1‑141 (MQ=255)
                                                                                                                 tatgTACATGCTTATTACCTATAAACAGATTTATGTCGGTTGTTTATCCTGAAGCTTTGTACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAGATTCGTGa                 <  1:560922/138‑1 (MQ=255)
                                                                                                                  aaGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGat                >  1:448000/1‑141 (MQ=255)
                                                                                                                  aaGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGag                <  2:91374/141‑2 (MQ=255)
                                                                                                                     tACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatata             >  2:59698/1‑141 (MQ=255)
                                                                                                                       cATGCTTATTACCTATGAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataat           <  1:248307/141‑1 (MQ=255)
                                                                                                                        aTGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataata          <  1:442359/141‑1 (MQ=255)
                                                                                                                          gCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataatata        <  1:768194/141‑1 (MQ=255)
                                                                                                                          gCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataatata        <  1:101484/141‑1 (MQ=255)
                                                                                                                          gCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCAGGTAAAGTAAATTCGTGatataatata        >  2:715775/1‑141 (MQ=255)
                                                                                                                           cTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACTTCCTAATTAGAAGTTGAGGCTAACATAATTAGTTTTATAGTAGAATGAGAATATTTATCCTATGACGTAGATTCCTAatatactataa       >  2:136922/1‑140 (MQ=255)
                                                                                                                           cTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAg       >  2:21451/1‑141 (MQ=255)
                                                                                                                                taCCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAATCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAat  <  2:93047/141‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
GGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAAT  >  CP000730/707745‑708013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑