Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,504,989 T→C 10.0% intergenic (+293/+111) USA300HOU_2365 → / ← gltT hypothetical protein/DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,504,9890TC10.0% 149.8 / 7.8 73intergenic (+293/+111)USA300HOU_2365/gltThypothetical protein/DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter
Reads supporting (aligned to +/- strand):  ref base T (26/38);  new base C (2/6);  total (28/45)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

GCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGACCTT  >  CP000730/2504852‑2505128
                                                                                                                                         |                                                                                                                                           
gcggATGCATAAAGGCGCCTAACAACCTGAGGTCACTCCGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTccc                                                                                                                                          <  2:123257/141‑1 (MQ=11)
gcgcATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTAGGGGTCGGTTCGCCTAAGATTTAGCGGATGCGTAAAAGTgcc                                                                                                                                          >  2:80425/1‑141 (MQ=14)
     tgcatAAAAGTCCCTAACCACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaaca                                                                                                                                     <  1:275045/141‑1 (MQ=11)
     tgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaaca                                                                                                                                     <  1:237681/141‑1 (MQ=255)
        atAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAAcc                                                                                                                                  <  2:134595/141‑1 (MQ=255)
         tAAACGCCCCTAACCACCTGGGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCt                                                                                                                                 <  2:209029/141‑1 (MQ=255)
               tcccTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCCAACAACCTGAGGTCACAAGGCGTCCAATCGCCGACGCTGTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGtt                                                                                                                           >  2:102410/2‑140 (MQ=11)
               ccccTAACAACCGGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTc                                                                                                                           <  1:220136/141‑1 (MQ=255)
                    aacaacCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTAc                                                                                                                      >  1:276983/1‑141 (MQ=255)
                      caacCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCGGAGGTTACTAGGc                                                                                                                    >  2:150695/1‑141 (MQ=255)
                        acCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCtt                                                                                                                  >  1:196260/1‑141 (MQ=255)
                          cTGAGGTCACTACGCCTCCGTTCCCCTGCGCTTTAGCGCATGCATAAAAGCCCCCAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTcc                                                                                                                <  2:107481/141‑1 (MQ=14)
                           tGAGGTCACTACGCCTCCGTGCGCCTGCGTTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTCTAGCGAATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCg                                                                                                               <  1:223591/141‑1 (MQ=14)
                              ggTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAAGAACCTGAGGTCACTACGCTTCCGTTc                                                                                                            >  1:175146/1‑141 (MQ=255)
                              ggTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCCGAGGTTACTGCGGTTCCGTTc                                                                                                            >  2:241142/1‑141 (MQ=14)
                               gTCACTCCGCTTCGGTTCGGCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCg                                                                                                           <  1:123365/141‑1 (MQ=14)
                               gTCACTACGCTCCCGGTCGCCGGCGCTTTTGCGCATGCATAAAAGCCCCTAACAACCTAAGATCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCg                                                                                                           <  1:158766/141‑1 (MQ=14)
                                tCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGc                                                                                                          >  2:147151/1‑141 (MQ=255)
                                 cACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTGGcc                                                                                                         >  1:241569/1‑141 (MQ=255)
                                  aCTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCt                                                                                                        >  1:227438/1‑141 (MQ=255)
                                    cccgcTTCGGGTCGGCCGCGCTTTAGCGCATGCATAAAAGCCCCTACCAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTgc                                                                                                      <  1:11775/139‑1 (MQ=11)
                                     cacttcgcgTTCGCCGTGCTTTTGCGCATGCATAAAAGCCCCTAACCACCTGAGGTCACTGCGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTgcgc                                                                                                    <  2:46019/133‑1 (MQ=11)
                                      cgcTTCCGTTCCCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTCAGCTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTgcgc                                                                                                    <  1:222289/141‑1 (MQ=14)
                                      cgcTCCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTgcgc                                                                                                    <  2:189178/141‑1 (MQ=255)
                                       gcTTCGGTCCGCCTGCGTTTCTGCGCGTGCATAAAGGCTCCTAACAACCTGGGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCt                                                                                                   <  2:232160/141‑1 (MQ=14)
                                        ttgctgtgccccTGCGCTTTGCGTATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCttt                                                                                                 <  1:51625/131‑1 (MQ=11)
                                        cttgggTTCGCCCTGGCCTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCtt                                                                                                  <  1:163596/137‑1 (MQ=14)
                                        cTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCtt                                                                                                  <  1:130605/141‑1 (MQ=255)
                                         tCCGGTTCGCCCGCGCTGTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCttt                                                                                                 <  1:106333/141‑1 (MQ=255)
                                           ccgTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAg                                                                                               <  2:227438/139‑1 (MQ=255)
                                           cGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCGCTACGCGTCGGATCGCCTGCGCTTTat                                                                                               >  2:274366/1‑140 (MQ=11)
                                               tCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgca                                                                                           >  1:153708/1‑141 (MQ=255)
                                               tCGCCTGCGCTTTAGCGCATGCAGAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCgca                                                                                           <  1:3307/141‑1 (MQ=11)
                                               gcGCCTGCGCCTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCgca                                                                                           <  1:165115/140‑1 (MQ=11)
                                                  ccTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcatgc                                                                                        >  1:76440/1‑141 (MQ=255)
                                                    tctgcTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTCCGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcatgcat                                                                                      <  2:155351/138‑1 (MQ=255)
                                                    tGCGCTTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcatgcat                                                                                      <  2:205030/141‑1 (MQ=255)
                                                    tGCGCTTATGCGCTTGCATAAAAGCCCCGAACCACCTGAGCGCACTACCCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAGGTCCCTAACAACCCGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcatgcat                                                                                      <  2:121890/141‑1 (MQ=14)
                                                        cTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATaaaa                                                                                  >  1:159394/1‑141 (MQ=255)
                                                                cagtcatAAAAGCCCCGAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaa                                                                          <  1:46652/137‑1 (MQ=255)
                                                                  tgcatAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTGCTGTTCGCCGGCGCGTTAGCGCAAGCAGGAAAGCCCCTaaca                                                                        >  2:256950/1‑141 (MQ=11)
                                                                   gcatAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaacaa                                                                       <  2:226384/141‑1 (MQ=255)
                                                                    catAAACGCCCCTAACAACCTGAGGTCCCTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTCCGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaacaac                                                                      <  1:98448/141‑1 (MQ=255)
                                                                     atAAAAGCCCCTAACAACCTGGGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCTCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAAcc                                                                     <  1:38081/141‑1 (MQ=255)
                                                                      tAAAAGCCCCTAACAACCTGAGGTTTCTGCGCCTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCCTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAAcct                                                                    >  1:68694/1‑140 (MQ=11)
                                                                       aaaaGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTCCGCTTCCGTTCGCCTTCGCTTTAGCCCATGCATAAAAGCCCCTAACAAcctg                                                                   >  1:12740/1‑139 (MQ=255)
                                                                         aggCCCCCAACAACCTGAGGTCACTCCGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCTTGCATAAAAGCCCCTTACAAcctttg                                                                 <  2:177398/139‑5 (MQ=14)
                                                                         aaGCCCCTCACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTTACAAcctttg                                                                 <  1:256950/141‑5 (MQ=255)
                                                                            ccccTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAAcctgaggtc                                                              <  1:155779/141‑8 (MQ=255)
                                                                              ccTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGt                                                            <  1:80425/141‑1 (MQ=255)
                                                                              ccTAACAACCCGCGGCCACTACGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGt                                                            <  2:255126/141‑1 (MQ=14)
                                                                               cTACCAACCTGGGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGGCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTa                                                           <  2:175146/141‑1 (MQ=255)
                                                                                 aacaacCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCCGAGGTCGCTACGCTTCCGTTCGGCTGCACATTAGCGCATCAATAGACGCCGCTGACAACCCACGGTTGTAAg                                                         >  2:287374/1‑141 (MQ=17)
                                                                                 aacaacCTGAGGGCACTACGCATCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAg                                                         >  1:148558/1‑141 (MQ=255)
                                                                                     acCTTACGTCACTTCCCTCCGCTTCGCCTGCGCTTTAGCGCATGCAGAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGc                                                     <  2:135653/141‑1 (MQ=14)
                                                                                       cggAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCtt                                                   <  1:241142/139‑1 (MQ=255)
                                                                                          aGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTACGGTTGTCACGGGCtcgtt                                                >  2:87998/1‑137 (MQ=14)
                                                                                           ggTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAGGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTAAGGTTgctacgggtttgttt                                               >  2:116740/1‑127 (MQ=14)
                                                                                           ggTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTaaa                                               <  1:213235/141‑1 (MQ=255)
                                                                                               aCTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTc                                           >  1:54799/1‑141 (MQ=255)
                                                                                                  aCGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGa                                        <  2:76440/141‑1 (MQ=255)
                                                                                                        cGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGACCCGCTTTTCTTGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAt                                  >  1:66435/1‑141 (MQ=21)
                                                                                                                   cgcTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAATGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTg                       <  1:150695/141‑1 (MQ=255)
                                                                                                                      tttAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGAtt                    >  2:252060/1‑141 (MQ=255)
                                                                                                                       ttAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGAttt                   >  1:276145/1‑141 (MQ=255)
                                                                                                                         aGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTa                 >  2:106132/1‑141 (MQ=255)
                                                                                                                            gcatgcatAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTTATTTTAAat              <  1:210402/141‑1 (MQ=255)
                                                                                                                            ccatgcatCAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCCTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAat              <  2:238157/140‑1 (MQ=255)
                                                                                                                               tgcatAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATAtt           <  2:241569/141‑1 (MQ=255)
                                                                                                                                   tAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTg       >  1:11495/1‑141 (MQ=255)
                                                                                                                                   tAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTg       >  1:76696/1‑141 (MQ=255)
                                                                                                                                    aaaaGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGa      <  1:116740/141‑1 (MQ=255)
                                                                                                                                        gTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGACCtt  <  1:87998/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGACCTT  >  CP000730/2504852‑2505128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: