Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,274,965 G→C 100% E106D (GAG→GAC bglG → transcriptional antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,274,9650GC100.0% 80.9 / NA 24E106D (GAG→GACbglGtranscriptional antiterminator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/14);  total (10/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAAAAAAGGGCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAGCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCATTTACT  >  CP000730/2274827‑2275101
                                                                                                                                          |                                                                                                                                        
caaaAAAGGGCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAccc                                                                                                                                        >  1:254535/1‑141 (MQ=255)
      aGGGCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTaa                                                                                                                                  >  1:163824/1‑141 (MQ=255)
                            gATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGaa                                                                                                            >  1:169074/1‑141 (MQ=255)
                                         aCGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTACAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTg                                                                                               <  1:222350/141‑1 (MQ=255)
                                          cGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGt                                                                                              <  2:112249/141‑1 (MQ=255)
                                             tttGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCa                                                                                           <  2:193216/141‑1 (MQ=255)
                                              ttGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGCCGTTTCTGTCCAg                                                                                          >  2:53213/1‑141 (MQ=255)
                                                  aGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACtt                                                                                      <  2:22940/141‑1 (MQ=255)
                                                  aGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACtt                                                                                      <  1:229425/141‑1 (MQ=255)
                                                     aaTCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTCCTGTCCAGACTTTAg                                                                                   <  1:212985/141‑1 (MQ=255)
                                                       tCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCAGTCCAGACTTTAGCa                                                                                 <  1:213282/141‑1 (MQ=255)
                                                                   caTCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAgat                                                                     >  2:68400/1‑141 (MQ=255)
                                                                         aCCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTa                                                               <  2:220158/141‑1 (MQ=255)
                                                                             ttGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGc                                                           <  2:167428/141‑1 (MQ=255)
                                                                             ttGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATGGGCGTTTCTGTCCGGACTTTAGCAAAGATGTTAGATGATTTAGGGc                                                           >  1:44222/1‑141 (MQ=255)
                                                                                       tGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTaaa                                                 >  2:42752/1‑141 (MQ=255)
                                                                                               agCAGAAAGCAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTAcc                                         <  1:25541/141‑1 (MQ=255)
                                                                                                gCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCa                                        >  1:109182/1‑141 (MQ=255)
                                                                                                                 tataCGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTAATCTCGaa                       >  1:146958/1‑141 (MQ=255)
                                                                                                                 tataCGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTAGCGTAAGCTCGaa                       >  2:58439/1‑141 (MQ=255)
                                                                                                                     cGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCg                   <  1:48928/141‑1 (MQ=255)
                                                                                                                       cTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTg                 >  2:66073/1‑141 (MQ=255)
                                                                                                                            aTACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGaa            <  1:100856/141‑1 (MQ=255)
                                                                                                                                      aGGACCCCGTTAAACAATGTAGTTTAGCGCAAGAAATTGTGCTTTCTGTCCACACTTTAGTAAACAGGTTAGATGAATTATGGCTTGATTTAAATAATTACCTTCTATTTTGATCTCGTAAGCTTGTCTAAGGCATTTACt  <  2:10673/141‑1 (MQ=255)
                                                                                                                                      aGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCGCGAAAGCGTAGCGAAGGCATTTACt  <  1:143909/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
CAAAAAAGGGCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAGCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCATTTACT  >  CP000730/2274827‑2275101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: