Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 638,614 G→T 100% T14K (ACA→AAA)  thiD ← phosphomethylpyrimidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730638,6140GT100.0% 77.4 / NA 25T14K (ACA→AAA) thiDphosphomethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/16);  total (9/16)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAA  >  CP000730/638478‑638749
                                                                                                                                        |                                                                                                                                       
aTCCATTGGTAATGGTATAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTc                                                                                                                                     >  1:169664/1‑141 (MQ=255)
  ccATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGAGCAAGCACTTTTGTCAg                                                                                                                                   >  1:287013/1‑141 (MQ=255)
        gTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCgg                                                                                                                             <  1:104600/141‑1 (MQ=255)
                                     tCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCAtt                                                                                                <  1:71474/141‑1 (MQ=255)
                                     tCTTGATCCATAGCAACGAGGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCAtt                                                                                                <  2:231390/141‑1 (MQ=255)
                                       tttATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGa                                                                                              >  2:262458/1‑141 (MQ=255)
                                         tATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGc                                                                                            <  1:91436/141‑1 (MQ=255)
                                         tATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGc                                                                                            <  1:50020/141‑1 (MQ=255)
                                         tATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGc                                                                                            <  1:269384/141‑1 (MQ=255)
                                                        tGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataa                                                                             >  1:277058/1‑141 (MQ=255)
                                                         ggCAGTTAAAGCGACCATGACATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCCGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataat                                                                            >  1:152417/1‑141 (MQ=255)
                                                               tAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATAtt                                                                      <  2:191080/141‑1 (MQ=255)
                                                                    cGACAATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTCAAAGCCATTGAGCTTCACTCCTACCTAATAATATTGTAtt                                                                 >  1:202266/1‑141 (MQ=255)
                                                                       ccATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAAACGGCAATTGTTAAAACTTTCTGTAAAGCCATTGAGCTTAACTCCTACATACTAATATTGTAtttac                                                              >  2:173853/1‑138 (MQ=255)
                                                                                   tATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAttttt                                                  <  1:166984/141‑1 (MQ=255)
                                                                                          ttCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTaa                                           <  2:168672/141‑1 (MQ=255)
                                                                                                gAACGTTTTCAACTCTTCTTGCATACCTGCGCCAGCACTTTTGTCAGACTCCGCAATTGTTAAAACTTTCTTTAAATCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGaaa                                     <  2:151696/141‑1 (MQ=255)
                                                                                                    gTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAata                                 <  1:272784/141‑1 (MQ=255)
                                                                                                            aTCTGCTTGCATACCTGCCGCAGCGCTGTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCAt                         <  2:287013/141‑1 (MQ=255)
                                                                                                            aTCTGCTTGCATACCTAAAACAGCAATTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTAAAAAAAAAAAAAAAATATTAAGCAt                         <  2:216033/141‑1 (MQ=255)
                                                                                                                ggttGTATACCTGCTCCATCACTTTTGTCATATCCGGCTATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCGACATAATAATATTGTATTCTTCATATCATTTTTATCCTAATTCAAAAATATTAAACAtaaaa                     <  2:124781/139‑5 (MQ=255)
                                                                                                                             cgcCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGAttg        >  2:197569/1‑141 (MQ=255)
                                                                                                                             cgcCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTAAATATTTGATGAttg        <  1:212975/141‑1 (MQ=255)
                                                                                                                                  gCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGATCTTCACTCCTACATAATAATATTTTGTTCAGCATATCATTTTTTTCCTACTTGAAGAATGTTACGCATTCTATAATTGATGATGGTTGaa   >  1:54848/1‑141 (MQ=255)
                                                                                                                                   cACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGaaa  <  1:170056/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAA  >  CP000730/638478‑638749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: