Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,679,267	C→T	12.5%	intergenic (+139/‑250)	USA300HOU_2535 → / → USA300HOU_2536	hypothetical protein/possible secretory antigen

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,679,267	0	C	T	12.5%	182.5 / 4.8	71	intergenic (+139/‑250)	USA300HOU_2535/USA300HOU_2536	hypothetical protein/possible secretory antigen
Reads supporting (aligned to +/- strand):  ref base C (28/33);  new base T (8/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 8.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACATGAATACATGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATAT  >  CP000730/2679129‑2679404                                                                                                                                            |                                                                                                                                          tACATGAATACATGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCAt                                                                                                                                         >  2:251999/1‑141 (MQ=255)    aTGAATACATGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTAATTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCAtttt                                                                                                                                      <  2:169789/141‑1 (MQ=255)        gtACATTGGGTCTAATCATTTCATTTTAGTGCTCTGATTGCTGGGCGCTAGTTTTAATGTTATTGCTACATTTAGTATATGGTGATTTCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTatat                                                                                                                                  <  2:455205/140‑1 (MQ=255)        aTACATGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTatat                                                                                                                                  <  2:472607/141‑1 (MQ=255)          aCATGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATAttt                                                                                                                                >  1:533937/1‑141 (MQ=255)            aTGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTc                                                                                                                              >  1:106179/1‑141 (MQ=255)             tGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCCTTTTTTATTTTcc                                                                                                                             >  1:218035/1‑141 (MQ=255)              ggAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGATCCATGTACC‑TTTTTTTTAAATTATTTTTTATTTTccc                                                                                                                            >  2:139148/1‑141 (MQ=255)              ggAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTccc                                                                                                                            >  1:116673/1‑141 (MQ=255)              ggAGTCACATCATCTCATGCAAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTccc                                                                                                                            >  1:434210/1‑141 (MQ=255)                 gTCACCTCATCTCATGTGAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTATAATACTATTTTATATTTTCCCACt                                                                                                                         <  2:644813/141‑1 (MQ=255)                 gTCACAGCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACt                                                                                                                         <  1:570192/141‑1 (MQ=255)                  tCACATTAGCGCATGCTAGCGCTCTGATTCCAGGGCGCTATTTTGAAGGTGATTGCGGCATTTAGTATATTGTGATTGCTAGTTTCTTGCTGTGCGAGATCCTTGTACC‑TTTTTTTTTCCTTATTTTATATTTccctatta                                                                                                                        <  2:51290/141‑6 (MQ=255)                    acaTCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAATTCATTTTTTATTTTCCCACTAat                                                                                                                      >  2:521311/1‑141 (MQ=255)                     catcatCTCATGTTAGCACTCTGATTGCACGGCGTTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAACTCATTTTATATTTTCCCACTAata                                                                                                                     <  2:112006/141‑1 (MQ=255)                        catcTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATCTTTTCTTCCTATTATcc                                                                                                                  >  2:563837/1‑141 (MQ=255)                            tcATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTg                                                                                                              <  1:210929/141‑1 (MQ=255)                             cATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTAAC‑TTTTTTTTAAATCATTTTATATTTTCATCCTAATATCCGCtat                                                                                                             >  1:342137/1‑139 (MQ=255)                               tGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTa                                                                                                           <  1:311190/141‑1 (MQ=255)                               tGTTAGCGCTCAGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTa                                                                                                           <  1:6368/141‑1 (MQ=255)                                     cgcTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTaatcaat                                                                                                     >  1:181769/1‑141 (MQ=255)                                      gcTTTGGTTGGAGGGCGCTATTTTTTATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTaatcaatc                                                                                                    <  1:219260/141‑1 (MQ=255)                                      cctctGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTaatcaatc                                                                                                    <  1:375664/140‑1 (MQ=255)                                        cctGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCt                                                                                                  <  1:156090/140‑1 (MQ=255)                                           gATTGCAGGTCTCTATTTTTAATGTTATTGCTACATTTAGTATATTGTTATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGAc                                                                                               <  1:37078/141‑1 (MQ=255)                                            aTTGCAGGGCGCTATTATATATATAAATGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACa                                                                                              <  1:362270/141‑1 (MQ=255)                                              tGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATc                                                                                            <  2:434210/141‑1 (MQ=255)                                              tGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCACCCCGACTTCCTCTGTTAATCAATCCTGACATc                                                                                            <  1:324019/141‑1 (MQ=255)                                                   ggCGCTATTTTTAAGGTTATTGCTACATTGAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGt                                                                                       >  1:358272/1‑141 (MQ=255)                                                     cgcTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTAt                                                                                     <  2:461139/141‑1 (MQ=255)                                                        tATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCACTCCTGAAATCCTTGTATCat                                                                                  >  2:381206/1‑140 (MQ=255)                                                         aTTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACt                                                                                 >  1:218817/1‑141 (MQ=255)                                                           ttttAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTAt                                                                               <  1:602542/141‑1 (MQ=255)                                                           ttttAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGAAATCCTTGTATCACTAt                                                                               >  1:511388/1‑141 (MQ=255)                                                               aaTGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACa                                                                           >  1:35304/1‑141 (MQ=255)                                                                  gTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTCACATCCTTGTATCACTATGACaatt                                                                        >  1:62274/1‑141 (MQ=255)                                                                   tcaTTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACaatta                                                                       <  1:149001/139‑1 (MQ=255)                                                                           aCATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTTATTTATTTTATATTTTCCCACTAATATCCGCTTTTTATCAATCCTTAGATCCTTGTATCACTATGACCATTAATTGTTaa                                                               >  2:375460/1‑141 (MQ=255)                                                                            cATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTaaa                                                              <  2:230321/141‑1 (MQ=255)                                                                              tttAGTATATTGTGATTGCTATTTTCTGTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACGAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATa                                                            <  1:590419/141‑1 (MQ=255)                                                                                  gTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATg                                                        >  1:498022/1‑141 (MQ=255)                                                                                      atTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAAtt                                                    >  1:365710/1‑141 (MQ=255)                                                                                          tgATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTTTATTTTTTTTTATTTACTCACTAATATCCTTTTTTCATTATTTATGGTGTTCTTGTATCAATATGACAATTTATTATCAAATACATGTAtttttt                                                >  2:153183/1‑138 (MQ=255)                                                                                           gATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTTTTTTTTTTTATATTTTCCCACTAATATCCGCTGTTAATCGATCCTGACGTCCTTGTATTCCTCTGCCCATTAATTGTTAAATACATGAGTTTTTAc                                               >  2:193751/1‑141 (MQ=255)                                                                                              tGCTATTTTCTTTCAGTTATAGAACCATGTACCTTTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACAt                                             >  1:577413/1‑141 (MQ=255)                                                                                               gCTATTTTCTTTCAGTTATAGAACCCTGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACTATTAATTGTTAAATACATGAATTTCTACAttt                                           >  2:287109/1‑141 (MQ=255)                                                                                                  aTTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTTTTTTTTTTGATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGGCATCCTTGTATCACTATTTCAATTAATTGTTAAATACATTTATTTTTACTTTTTAt                                        >  2:426893/1‑141 (MQ=255)                                                                                                  aTTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTTTATTTTTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTCTCACTATGTCCATTAATTGTTAAATACATTTATTTCTACGTTTTAt                                        >  2:8347/1‑141 (MQ=255)                                                                                                  aTTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATGGTTAAATACATGAACTTCTACATTTTAt                                        >  2:326895/1‑141 (MQ=255)                                                                                                   ttttttttCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATg                                       <  2:512887/141‑1 (MQ=255)                                                                                                   tttgCTTTTCGTTCTACATCCCTGTTCC‑TTTTCTTTAAATCATTTTATCTTTTCCCCCTAATATCCGCTGTTAATCAATCCTGACATCCTTTTATCACTATGTCAAGTTATTGTTAAATACATGATTTTCTACATTTTATg                                       <  2:652565/141‑1 (MQ=255)                                                                                                        tttcAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGaaaaa                                  >  1:647470/1‑141 (MQ=255)                                                                                                         ttcACTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGTCAATTAATTGTTAAATACATGAATTTCTAAAATTaaaaaaaaaa                                 <  2:358272/141‑1 (MQ=255)                                                                                                          tcAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAAt                                >  1:68742/1‑141 (MQ=255)                                                                                                          tcAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTAACCATAAAACCATTGATATAGAAAAACATGAATTTCTACATTTTATGAAAAAAt                                >  2:303033/1‑141 (MQ=255)                                                                                                          tcAGTTATAGAACCATGTACC‑TTTTTTTT‑AATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATc                               <  1:176140/141‑1 (MQ=255)                                                                                                                  aGAACCATGTACC‑TTTTTTTTTTATTTTTTTATATTTTCCCACTAATATCCGCTTTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTATATTTTATGAAAAATTCTAttttt                        >  2:553508/1‑141 (MQ=255)                                                                                                                     aCCATGTACC‑TTTTTTTTAAATCATTTTATTTTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTCATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTttctt                     <  2:491895/141‑1 (MQ=255)                                                                                                                       cttGTTCC‑TTTTTTTTAAATCATTTTACTTCTACCCACTAATATCCGCTCTTAATCAATCCTGACATTCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTAc                   <  1:172869/139‑1 (MQ=255)                                                                                                                       cATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTAc                   <  1:202069/141‑1 (MQ=255)                                                                                                                         tGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCAATTTTTATTACaa                 >  1:585876/1‑141 (MQ=255)                                                                                                                           tACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAAtt               >  1:263929/1‑141 (MQ=255)                                                                                                                           tACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAAtt               <  1:206280/141‑1 (MQ=255)                                                                                                                            aCC‑TTTTTTTTTTTTTTTTTTATATTTTGCCGCCAATATCCGCTGTTAATGAATCCTGACGTCCTTGTATCACTATTACAATTAATTGTTAAATACATGCATTTTTTCATTTTTTGATAAATTCCATTTTTATTACAAttt              >  2:196297/1‑140 (MQ=255)                                                                                                                            aCC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATGCATTTTTATTACAAtta              >  1:260603/1‑140 (MQ=255)                                                                                                                            aCC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTc              <  1:251999/141‑1 (MQ=255)                                                                                                                            aCC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTc              <  1:574069/141‑1 (MQ=255)                                                                                                                              c‑TTTTTTTTAAATCATTTTGTATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAGAAATCCATTTTTATTACAATTCaa            <  1:426893/141‑1 (MQ=255)                                                                                                                                     tttAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACttt      >  1:144637/1‑141 (MQ=255)                                                                                                                                      ttAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTATACATTGTATGAAAAAATCCATTTTTATTACAATTCAACACTTta     <  1:459333/141‑1 (MQ=255)                                                                                                                                         aaTCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTatat  <  2:196570/141‑1 (MQ=255)                                                                                                                                            |                                                                                                                                          TACATGAATACATGGAGTCACATCATCTCATGTTAGCGCTCTGATTGCAGGGCGCTATTTTTAATGTTATTGCTACATTTAGTATATTGTGATTGCTATTTTCTTTCAGTTATAGAACCATGTACC‑TTTTTTTTAAATCATTTTATATTTTCCCACTAATATCCGCTGTTAATCAATCCTGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATAT  >  CP000730/2679129‑2679404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑