Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,413 C→T 51.6% V47I (GTC→ATC)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,4130CT51.6% ‑3.5 / inf 362V47I (GTC→ATC) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (80/93);  new base T (81/103);  total (164/198)
Fisher's exact test for biased strand distribution p-value = 7.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.25e-01

Only 100 of 365 total aligned reads displayed.

AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCAT  >  CP000730/36273‑36551
                                                                                                                                            |                                                                                                                                          
aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTATCATCAACACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTTAACCCAACGGTAGAc                                                                                                                                            >  2:141151/1‑141 (MQ=255)
  tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCg                                                                                                                                          <  1:186729/141‑1 (MQ=40)
     cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTg                                                                                                                                       <  1:142093/141‑1 (MQ=255)
      actatatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCCTTATCATGAACCCAACGGTAGATCGTTGa                                                                                                                                      <  1:324413/138‑1 (MQ=11)
      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTTTTTTTTTTTCCAAATTTGATATAAAATTGGGGCATATTCTTGATATAAACGGTAGACGGTTgg                                                                                                                                      >  2:431612/1‑140 (MQ=11)
      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                                                      >  1:650129/1‑141 (MQ=21)
       cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                                                                                                                     <  1:588062/141‑1 (MQ=40)
       cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTTTTTTTTTTATATACAATCAGGCCATATTCTTTCACCCAGCGGTAGAGGGTTGaa                                                                                                                                     >  2:534346/1‑141 (MQ=2)
        gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAat                                                                                                                                    <  1:531579/141‑1 (MQ=255)
        gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATAAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCGAAATTTGATATAAAATTGGGGCATATTCTTGAACCAAACGGTAGAGCGTTGAat                                                                                                                                    >  2:547354/1‑141 (MQ=11)
         gtatcAATAGCTCCAGTTGCCTTTTATTTTGATGTACGCCTCATCAATACGCCAGTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatg                                                                                                                                   <  1:89311/140‑1 (MQ=11)
         atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCTTATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                                                   <  1:479153/141‑1 (MQ=11)
          tataAATAGCTCCATTTTCCTTTTATTTTGATATTCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                                                  <  1:39825/141‑1 (MQ=255)
            taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                                                                                                                                <  2:298259/141‑1 (MQ=255)
             aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                                                               <  2:399789/141‑1 (MQ=255)
              aaTAGCTCCATTATCCTTCTATTTTGATATACCTTTCATCAATACGCCATTTGTAATAACCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAATTTGGGGCATATTCTTGAACCCAACGGTATATCGTTGAATGATaaa                                                                                                                              >  2:24872/1‑141 (MQ=11)
                 aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                                                                                                                           <  2:269275/141‑1 (MQ=255)
                  tttCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                                                          <  2:496060/139‑1 (MQ=255)
                     ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGGATGATGAACGTTTac                                                                                                                       <  1:337554/141‑1 (MQ=255)
                      ttttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGTTTTTTCTTCCAAAGTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                                      <  2:508381/139‑1 (MQ=11)
                      cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGTGGCATATTCTTAAACCCAACAGTAGACCGTTCAATGATGACCGTTTcca                                                                                                                      >  2:52848/1‑141 (MQ=11)
                      cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                                      >  1:438965/1‑141 (MQ=255)
                       atattCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                                                     <  1:19916/138‑1 (MQ=21)
                        ttttCCTTTTATTTTGATGTACGTCTTATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                                                    <  1:404793/141‑1 (MQ=21)
                        ttttCCTTTTATTTTGATATACGTCTCACCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                                                    <  2:290055/141‑1 (MQ=255)
                        ttttCCTCGTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                                                    <  1:12891/141‑1 (MQ=11)
                         tttCCTTTTATTTTTATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                                                                   <  1:420595/141‑1 (MQ=11)
                         tttCCTTTTATTTTGATGTACGTCTCATCACTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGCACCTGACGGTAGACCGTTGAATGATGACCGTTTAcacca                                                                                                                   >  2:626121/1‑141 (MQ=11)
                         tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacaacc                                                                                                                   >  2:634857/1‑138 (MQ=255)
                          ttCCTTTTATTTTGTTTTACGTCTCATCAATACGCCATTTGTAATACGCCTATTTATTCTTTGTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                                                                                                                  <  1:317795/141‑1 (MQ=11)
                          ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                                                                                                                  >  1:109458/1‑141 (MQ=25)
                           tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                                                                                                                 <  1:267849/141‑1 (MQ=40)
                            ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                                                                >  1:100640/1‑141 (MQ=21)
                            ccTTTTATTTTGATGTACGTCGCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAGATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                                                                >  1:344940/1‑141 (MQ=255)
                                ttATTTTGGTATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGGAGATCGTTGAATGATGAACGTTTACACCACGTTctc                                                                                                            <  2:609394/141‑3 (MQ=11)
                                 tATTTTGATGTACGTCTCATCAATACGTCATTTGGAATAAGCTTTTTTATCCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGTACCTAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                                                           <  2:438095/141‑1 (MQ=11)
                                 tATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct                                                                                                           <  1:627536/141‑4 (MQ=255)
                                  aTTTTGATATACGTCTCACCAATACGCCATTTGTAATATGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACTCCACGTTCTCtt                                                                                                          <  2:230969/141‑1 (MQ=11)
                                    tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCTATTTTTTATATAAAATTGGGGCATATTCTTTAACCCAACGGTAGACGGTTGAATGATGAACGTTTCCACCACGtcgattttt                                                                                                        >  2:283483/1‑133 (MQ=11)
                                    tgtGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                                                        <  2:425497/139‑1 (MQ=21)
                                      tggtGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAata                                                                                                      <  1:3520/138‑1 (MQ=21)
                                       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                     >  2:516674/5‑141 (MQ=255)
                                       gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGCTGAACGCTTACACCAAGTTTCCTTAatat                                                                                                     >  2:361700/1‑141 (MQ=11)
                                         tGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAttt                                                                                                   <  1:209792/141‑1 (MQ=18)
                                          gTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc                                                                                                  <  1:640928/141‑1 (MQ=39)
                                          atACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTc                                                                                                  <  1:121433/140‑1 (MQ=255)
                                           tACGTCTCATCAATACGCCATTGGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGATTGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                 <  1:462229/141‑1 (MQ=255)
                                             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                                                               <  1:592702/141‑1 (MQ=255)
                                             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                                                               <  2:416648/141‑1 (MQ=255)
                                             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACAGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                                                               <  2:334692/141‑1 (MQ=18)
                                             cGTCTCACCAATACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                                                               <  1:445116/141‑1 (MQ=255)
                                              gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGat                                                                                              <  1:158529/141‑1 (MQ=21)
                                                 tacttaacaCGCCCTGTGTAATGATCTTTTTTACGCTTCTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATTAACGTTTACACCACGTTCCCTTAATATTTCAGatata                                                                                           <  1:277941/133‑1 (MQ=11)
                                                  catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                                                          <  2:380302/141‑1 (MQ=255)
                                                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTAAATATTTCGTATATGTc                                                                                         >  1:234358/1‑141 (MQ=11)
                                                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGTTCTCTTAATATTTCAGATATATcc                                                                                        >  2:168853/1‑140 (MQ=255)
                                                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                                                        <  1:546038/141‑1 (MQ=21)
                                                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTCCACCACGATCTCTTAATATTTCCGATATATTAc                                                                                       >  1:136776/1‑141 (MQ=11)
                                                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                                                       >  2:127539/1‑141 (MQ=40)
                                                      aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGAAATATCACg                                                                                      >  2:601754/1‑141 (MQ=21)
                                                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACgt                                                                                     >  1:169473/1‑140 (MQ=25)
                                                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                                                     <  2:569016/141‑1 (MQ=18)
                                                         aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                                                   <  2:117203/141‑1 (MQ=255)
                                                         aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTACTATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                                                   >  1:297142/1‑141 (MQ=11)
                                                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTGCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                  <  2:73041/141‑2 (MQ=11)
                                                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                                                              >  1:492423/1‑141 (MQ=255)
                                                               tttGTTATAAGCTTTTTTATGATTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                             <  1:8243/141‑1 (MQ=11)
                                                               tttGTAATAAGCTTTTTTATGTTTTTTTTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                             <  1:292667/141‑1 (MQ=11)
                                                               tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                             <  2:650129/141‑1 (MQ=18)
                                                               tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                             >  1:285297/1‑141 (MQ=21)
                                                                ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                                                                 <  1:535204/104‑1 (MQ=255)
                                                                ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                            >  1:202949/1‑141 (MQ=255)
                                                                 ttgaataaGCTTTTTTATGCGTTGTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAGCCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                           <  1:120111/138‑1 (MQ=11)
                                                                 tggaataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGTATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                           <  2:258648/138‑1 (MQ=11)
                                                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTGGGGCATATTCTGGAACCCAACGGTAGATCCTGGAATTATCAACTTTTACACCACGTTCTCTTAATATTGCAGATATATCACGATAGCTCAATg                                                                           <  2:184949/141‑1 (MQ=11)
                                                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                           <  1:565053/141‑1 (MQ=11)
                                                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                           <  2:656752/141‑1 (MQ=255)
                                                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                           <  2:159218/141‑1 (MQ=255)
                                                                  gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                          <  1:603602/141‑2 (MQ=11)
                                                                  gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAACTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTGCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                          <  2:342761/141‑2 (MQ=11)
                                                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                         >  1:135236/1‑139 (MQ=11)
                                                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCGTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTGTTAATATTTTAGCTATATCACGATTGCTCAAttca                                                                         >  2:558031/1‑138 (MQ=11)
                                                                    aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACGCCACGTTCCCTTAATATTTCAGCTATATCACGACAACTCAATGtat                                                                        >  1:51926/1‑141 (MQ=255)
                                                                           ttttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAg                                                                 >  1:663701/1‑141 (MQ=35)
                                                                               ttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTACCTTAATATTTCATGTATATCACGATAACTCAATGTATATCTTAGAtca                                                             >  2:464318/1‑139 (MQ=255)
                                                                                    cTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGcc                                                        <  1:44918/141‑1 (MQ=35)
                                                                                     tttttCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCa                                                       <  2:450838/141‑1 (MQ=35)
                                                                                        ttcttcCAAATTTGATACAAAATTGGGGCATATTCTTGCACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACg                                                    >  1:429423/1‑141 (MQ=25)
                                                                                                 aTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTg                                           <  1:395460/141‑1 (MQ=35)
                                                                                                      aTAGAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATACCTCAATGTATATCTTAGACAGTAGCCAACTGCTACAGTGATAAc                                      <  1:367385/141‑1 (MQ=255)
                                                                                                          aaaaTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATcc                                  >  2:111524/1‑141 (MQ=32)
                                                                                                               tGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGtt                             <  2:373085/141‑1 (MQ=32)
                                                                                                                 gggCATATTCTTGTACCCAAGGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTATTATTTCTGATATATCTCGATACCTCAATGTATATTTTTGATAGTAGCCAACGGCTACAGTGATAACATCCTTGttta                           >  2:312935/1‑139 (MQ=11)
                                                                                                                  ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACAACCTTGGTaaa                          >  2:484384/1‑141 (MQ=17)
                                                                                                                       atTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGtt                     <  1:255970/141‑1 (MQ=32)
                                                                                                                         tCTTGCACCTAAAGGTAGACCGTTGAATCATGAACGTTTACACCACGTTCCCTTACTATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAACTGTTta                   <  2:213094/141‑1 (MQ=11)
                                                                                                                                 ccAACCGTAGACCGTTGCATGACAATCGTTTGCCCCACGTTGTCTTTTTTTATCATATATATCACCTTTACTCAAGGTTTTTTTTACTTAGTATCCGATTTCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaa           <  2:57934/141‑1 (MQ=2)
                                                                                                                                   caCGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCGTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAAt         <  1:548927/140‑1 (MQ=17)
                                                                                                                                         gcaCCGTGGAATGATGAACGTTTACACCACGTTCCCTTAAGATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCa   <  2:100760/139‑1 (MQ=12)
                                                                                                                                          gACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATCTCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCGGAAATGGTTCAt  >  1:19393/1‑141 (MQ=12)
                                                                                                                                            |                                                                                                                                          
AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCAT  >  CP000730/36273‑36551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: