Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,013,179 A→T 30.2% Q19H (CAA→CAT)  USA300HOU_1864 → possible metal‑dependent hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,013,1790AT30.2% 40.8 / 21.7 33Q19H (CAA→CAT) USA300HOU_1864possible metal‑dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base A (14/9);  new base T (4/6);  total (18/15)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

CATGGTTAGTATGTAAGTTAATTTAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTCGCATCATAGAGGTATCACGCA  >  CP000730/2013039‑2013313
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cATGGTTAGTATGTAAGTTAATTTAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCaa                                                                                                                                        >  2:16757/1‑141 (MQ=255)
         tATGTAAGTTAAATTAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCa                                                                                                                               <  1:152860/141‑1 (MQ=255)
                ttaaaTTTAGGTATTTGCAAAATTGGATTATAATAGTATATATAATATTATGAACTGAGCGGTCGGTGATGGACACTGCAACACTTATCGCAATTGGGGTGGGCCTTACATCACTTGCAACTCATGATCCAGCAATTGCtt                                                                                                                        <  2:122256/138‑1 (MQ=255)
                gTTAATTTAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCGACTCAAGATCCAGCAATGGCtt                                                                                                                        <  2:252009/141‑1 (MQ=255)
                      ttAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGt                                                                                                                  >  2:8487/1‑141 (MQ=255)
                      ttAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGt                                                                                                                  >  1:107280/1‑141 (MQ=255)
                       tAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCATGATCCAGCAATGGCTTCTACGtt                                                                                                                 >  2:127694/1‑141 (MQ=255)
                        aGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAAATGATATAAACACGGCAAAACATATTTCAATTGGGGTAGAGCGTACAGCACTTGCAACTCAACATCCAGCAACAGGTTCTACGttt                                                                                                                >  1:241981/1‑141 (MQ=255)
                             tttGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTGTACGTTTGGTGc                                                                                                           >  1:279753/1‑141 (MQ=255)
                                  gAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCATGATCCAGCAATGGCTTCTACGTTTGGTGCAACAg                                                                                                      <  2:100890/141‑1 (MQ=255)
                                         gATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCATGATCCAGCAATGGCTTCTACGTTTGGTGCAACACCTACAAc                                                                                               >  1:256289/1‑141 (MQ=255)
                                         gATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAAc                                                                                               >  2:274030/1‑141 (MQ=255)
                                                         ataataTTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTATGGAGCAACAGATACAACACTTGTcctgcgtaca                                                                               >  2:181203/1‑132 (MQ=255)
                                                          taataTTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCAt                                                                              >  1:8981/1‑141 (MQ=255)
                                                              tttattaAAGGAGTGAACGGATATGGACACTGCAACACATATCGCAATTGTGGTGGGCCTTACTGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAATCCTTATTGTTGGTTCATTAAt                                                                          <  2:236365/140‑1 (MQ=255)
                                                              attatGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGCGGTGGGCCTTACGGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAAGCCTTATTGTTGGTTCATTAAt                                                                          <  2:236268/141‑1 (MQ=255)
                                                                   gAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAAGTCCTg                                                                     <  1:346618/141‑1 (MQ=255)
                                                                         aGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCATGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATgggg                                                               >  2:345295/1‑141 (MQ=255)
                                                                          gTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGa                                                              <  1:47591/141‑1 (MQ=255)
                                                                                 gATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTTATGGGGATACTGtt                                                       >  1:11073/1‑141 (MQ=255)
                                                                                 gATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAACAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCCTTGGGTAATTAATTCATGCTCGGCATACTGtt                                                       >  2:60163/1‑141 (MQ=255)
                                                                                   tatGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCt                                                     <  1:8487/141‑1 (MQ=255)
                                                                                         cacTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCATGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCttaaatt                                               >  2:27724/1‑141 (MQ=255)
                                                                                             gCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAg                                           >  1:258800/1‑141 (MQ=255)
                                                                                                  acaTATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACaa                                      <  1:157717/141‑1 (MQ=255)
                                                                                                      aaccataTTGGGTTGTGCCTTCTAGCACTTGCAACTCATCATCCAGCAATGGCTTCGACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCa                                  <  2:329471/135‑1 (MQ=255)
                                                                                                        cGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCATGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAAc                                <  1:201662/141‑1 (MQ=255)
                                                                                                                 ggTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTc                       >  1:277653/1‑141 (MQ=255)
                                                                                                                 ggTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTc                       >  1:47728/1‑141 (MQ=255)
                                                                                                                   tGGGCCTTACAGCACTTGCAACTCATGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTCGc                     <  1:345295/141‑1 (MQ=255)
                                                                                                                   tGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTCGc                     >  1:337994/1‑141 (MQ=255)
                                                                                                                         ttACAGCACTTGCAACTCATGATCCAGCAACGGCTTCTACGTTGGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTTAGGGGGACACTGTTCTTAAATTAAAGGACAATGCAACATATATTTCGCATCATa               <  1:330936/141‑1 (MQ=255)
                                                                                                                                      aaCTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGTACAATGCAACATATATTTCGCATCATAGAGGTATCACGCa  >  2:145359/1‑141 (MQ=255)
                                                                                                                                      aaCTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTCGCATCATAGAGGTATCACGCa  <  1:187645/141‑1 (MQ=255)
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CATGGTTAGTATGTAAGTTAATTTAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTCGCATCATAGAGGTATCACGCA  >  CP000730/2013039‑2013313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑