Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,761,889 C→A 78.6% D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,761,8890CA78.6% 182.5 / 47.7 97D388Y (GAT→TAT) folCtetrahydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base C (11/10);  new base A (42/34);  total (53/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

ATTCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTTC  >  CP000730/1761751‑1762026
                                                                                                                                          |                                                                                                                                         
aTTCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCAtata                                                                                                                                         >  1:262067/1‑141 (MQ=255)
 ttctgcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTTTAAATTCAACGTAGTCAtatat                                                                                                                                        >  2:918601/1‑140 (MQ=255)
   cttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATatacta                                                                                                                                      >  1:324783/1‑141 (MQ=255)
   cttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATATAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATGATTTTTTATAAATTCAACGTAGTCATatacta                                                                                                                                      >  1:709782/1‑141 (MQ=255)
     tcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAAt                                                                                                                                    <  1:844087/141‑1 (MQ=255)
       aTGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTCATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTg                                                                                                                                  <  1:25958/141‑1 (MQ=255)
        tGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGt                                                                                                                                 >  2:256519/1‑141 (MQ=255)
         ggCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTa                                                                                                                                >  1:48565/1‑141 (MQ=255)
          gCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTAt                                                                                                                               <  1:400830/141‑1 (MQ=255)
            tATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTAttt                                                                                                                             <  2:1055410/141‑1 (MQ=255)
             aTTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTCCAACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTtc                                                                                                                            <  1:559139/141‑1 (MQ=255)
             aTTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCTACGTAGTCATCTACTAATTGTATTtc                                                                                                                            <  2:658168/141‑1 (MQ=255)
             aTTTTAGTTTTAGTTTCATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACATACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAAGTCAACGTAGTCATATACTAATTGTATTtc                                                                                                                            <  1:635499/141‑1 (MQ=255)
                ttagttttagttTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTtcatc                                                                                                                         >  2:823701/1‑141 (MQ=255)
                ttagttttagttTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTtcatc                                                                                                                         >  2:537500/1‑141 (MQ=255)
                ttagttttagttTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTtcatc                                                                                                                         <  1:333867/141‑1 (MQ=255)
                  agttttagttttATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTcatcat                                                                                                                       <  2:910399/141‑1 (MQ=255)
                      ttagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCCTCATTTTc                                                                                                                   >  2:462156/1‑141 (MQ=255)
                         gttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAAt                                                                                                                <  2:284596/141‑1 (MQ=255)
                         gttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAAt                                                                                                                <  2:329621/141‑1 (MQ=255)
                          ttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAAta                                                                                                               >  1:366479/1‑141 (MQ=255)
                                        aaaGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATttc                                                                                                 >  1:908418/1‑141 (MQ=255)
                                         aaGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATttct                                                                                                <  2:825162/141‑1 (MQ=255)
                                          aGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATttctt                                                                                               >  2:194476/1‑141 (MQ=255)
                                          aGTCTAATTTTGATTTAACATCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCCTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATttctt                                                                                               >  1:791939/1‑141 (MQ=255)
                                           gTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATttcttc                                                                                              <  1:914447/141‑1 (MQ=255)
                                           gTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATttcttc                                                                                              >  2:595242/1‑141 (MQ=255)
                                           gTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATAATACCTACAATttcttt                                                                                              >  2:344028/1‑140 (MQ=255)
                                            tCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTtcttct                                                                                             >  2:898036/1‑141 (MQ=255)
                                            tCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTtcttct                                                                                             >  1:285097/1‑141 (MQ=255)
                                             cTAAATTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTcttctt                                                                                            >  1:724590/1‑141 (MQ=255)
                                                   ttGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTa                                                                                      <  1:840022/141‑1 (MQ=255)
                                                    tGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTaa                                                                                     <  1:971723/141‑1 (MQ=255)
                                                     gATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAAc                                                                                    >  2:341078/1‑141 (MQ=255)
                                                     gATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTCAc                                                                                    <  2:803686/141‑1 (MQ=255)
                                                     gATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAAc                                                                                    <  2:959538/141‑1 (MQ=255)
                                                     gATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAAc                                                                                    <  1:18079/141‑1 (MQ=255)
                                                      aTTTAACTTCACTTATTAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACg                                                                                   <  2:405306/141‑1 (MQ=255)
                                                          aaCTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCttt                                                                               >  1:404221/1‑141 (MQ=255)
                                                          aaCTGCACTTATGAAATGCAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCttt                                                                               >  2:1052897/1‑141 (MQ=255)
                                                           agttCACTTATGAAAGACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTc                                                                              <  2:413813/139‑1 (MQ=255)
                                                              tCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGa                                                                           >  1:660150/1‑141 (MQ=255)
                                                                 cTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGaaaa                                                                        >  1:1059814/1‑141 (MQ=255)
                                                                 cTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGaaaa                                                                        <  1:256519/141‑1 (MQ=255)
                                                                    aTGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATGCTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCa                                                                     >  2:806695/1‑141 (MQ=255)
                                                                    aTGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCa                                                                     >  2:960784/1‑141 (MQ=255)
                                                                      gAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATTATTTTTACATAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGCAACGCTTTCGGCAAATCaaa                                                                   >  2:200012/1‑141 (MQ=255)
                                                                      gAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGGAAATCaaa                                                                   >  1:616094/1‑141 (MQ=255)
                                                                      gAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCaaa                                                                   >  2:454650/1‑141 (MQ=255)
                                                                       aaaTACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTCCAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAAt                                                                  <  1:590050/141‑1 (MQ=255)
                                                                       aaaTACAGACTACCGGCAATTACTAACGTATCACCTTGATAATTTTTTATAAATTCAACGTATTCATATACTAATTGTATTTCATCATTTTCAAAACTACCTACACTCACCTTCGTGCGTAACGCTTTCGGAAAATCAAAt                                                                  <  1:513146/141‑1 (MQ=255)
                                                                          tACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCa                                                               >  2:639179/1‑141 (MQ=255)
                                                                           aCAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCCTTCTATGCACCACCAACAATATCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCat                                                              >  1:30719/1‑140 (MQ=255)
                                                                           aCAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATACAATTCAg                                                              >  2:944573/1‑141 (MQ=255)
                                                                            aaGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGt                                                             <  2:813013/140‑1 (MQ=255)
                                                                               aCTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTTTTTTTTTTTCGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTCCAATTTCTTTTTTGCGTAACGCTTTCGCAAAATCAAATCTAGTTgt                                                          >  2:255086/1‑140 (MQ=255)
                                                                                 tACCGTTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCAt                                                        <  1:13558/141‑1 (MQ=255)
                                                                                 tACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCAt                                                        <  1:161387/141‑1 (MQ=255)
                                                                                   ccGGTAATTACTTATGTATCACCTTGATAATTTTTTATCAATTCATCGTAGTCATATACTAATTGTAGTTCATGATTTTCAATACTACCTATTTTTTCTTCTTTGCGTAACTCTTTCGGAAAATCAAATTCAGTTGCATaa                                                      <  2:326312/141‑1 (MQ=255)
                                                                                       tAATTACTAATGTATCACCTTTATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACg                                                  <  1:1005645/141‑1 (MQ=255)
                                                                                         aTTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTa                                                >  1:623228/1‑141 (MQ=255)
                                                                                            aCTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgc                                             <  1:483839/141‑1 (MQ=255)
                                                                                            aCTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgc                                             >  2:671284/1‑141 (MQ=255)
                                                                                            aCTAATGTATCACCTTGATAATTTTTTACAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCCTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgc                                             <  1:9920/141‑1 (MQ=255)
                                                                                               aaTGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCa                                          <  1:1008906/141‑1 (MQ=255)
                                                                                               aaTGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAAAGATTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCa                                          >  2:288402/1‑141 (MQ=255)
                                                                                                    aTCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCTGAAAATCAAATTCAGTTGCATAAAACGTTTTCTCAATTaa                                     >  1:291777/1‑141 (MQ=255)
                                                                                                    aTCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAATGTATGTGCAATTaa                                     >  1:326558/1‑141 (MQ=255)
                                                                                                    aTCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTaa                                     >  1:854128/1‑141 (MQ=255)
                                                                                                      cACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAAc                                   >  1:836710/1‑141 (MQ=255)
                                                                                                       aCCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTCCAATTACTTCTTTGCCTAACGCTTTCGGAAAATCAAAGTCAGTTGCATAAAACGTATGCGCAATTAAACt                                  <  1:159581/141‑1 (MQ=255)
                                                                                                       aCCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGAGCAATTACACt                                  >  1:27414/1‑141 (MQ=255)
                                                                                                       aCCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGAATGCGCAATTAAACt                                  >  1:950965/1‑141 (MQ=255)
                                                                                                        ccTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACtt                                 <  2:836710/141‑1 (MQ=255)
                                                                                                         cTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTTCATAAAACGTATGCGCAATTAAACTTa                                >  2:243610/1‑141 (MQ=255)
                                                                                                         cTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTAATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGTAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTa                                >  1:3301/1‑141 (MQ=255)
                                                                                                          ttGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATTCGCAATTAAACTTaa                               >  1:350609/1‑141 (MQ=255)
                                                                                                           tGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCCTCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGAGCAATTAAACTTaaa                              >  2:685613/1‑141 (MQ=255)
                                                                                                           tGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTaaa                              >  1:518609/1‑141 (MQ=255)
                                                                                                              tAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGt                           >  1:259334/1‑141 (MQ=255)
                                                                                                               aaTTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGtt                          <  1:652067/141‑1 (MQ=255)
                                                                                                                aTTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGttt                         >  1:333188/1‑141 (MQ=255)
                                                                                                                aTTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGttt                         >  1:911394/1‑141 (MQ=255)
                                                                                                                 ttttttATAAATTCAACGTATTCATCTACTAATTGCATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACCCTTTCGGAAAATCATATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTg                        <  1:34132/141‑1 (MQ=255)
                                                                                                                 ttttttATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGATAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTg                        <  1:702547/141‑1 (MQ=255)
                                                                                                                 ttttttATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTg                        <  1:823709/141‑1 (MQ=255)
                                                                                                                 ttttttATAAATTCAACGTAGTCATATACTAATTGTATTTCAGCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTg                        <  2:284562/141‑1 (MQ=255)
                                                                                                                  tttttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAAGATATTCGCAATTAAAAGTCAAAGTTTGa                       >  2:477230/1‑141 (MQ=255)
                                                                                                                       ataAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACAACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtc                  >  1:998284/1‑141 (MQ=255)
                                                                                                                           aTTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGt              <  1:904108/141‑1 (MQ=255)
                                                                                                                            ttCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAAGCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCCCGtt             <  1:355270/141‑1 (MQ=255)
                                                                                                                             tCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTa            >  1:729080/1‑141 (MQ=255)
                                                                                                                             tCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAAGTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTa            <  2:908418/141‑1 (MQ=255)
                                                                                                                                 cGTAGTCATCTACCAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCg        <  1:35699/141‑1 (MQ=255)
                                                                                                                                 cGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTCCAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCg        <  2:366479/141‑1 (MQ=255)
                                                                                                                                 cGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAGAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCg        >  1:369312/1‑141 (MQ=255)
                                                                                                                                 cGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCg        >  2:778680/1‑141 (MQ=255)
                                                                                                                                    aGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGtt     >  1:932755/1‑141 (MQ=255)
                                                                                                                                    aGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGtt     >  2:36124/1‑141 (MQ=255)
                                                                                                                                       cATCTACTAATTGTATTTCATCATTTTCAATACCACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTTc  <  1:138113/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
ATTCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTTC  >  CP000730/1761751‑1762026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: