Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,910 A→G 83.4% intergenic (‑1/‑121) USA300HOU_0653 ← / → USA300HOU_0654 ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,9100AG83.4% 228.5 / 29.3 100intergenic (‑1/‑121)USA300HOU_0653/USA300HOU_0654ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (6/11);  new base G (49/34);  total (55/45)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAG  >  CP000730/707773‑708049
                                                                                                                                         |                                                                                                                                            
ttGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACTTGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGt                                                                                                                                          >  2:660767/1‑141 (MQ=255)
  taTAAGGGAAGATTGACCACGCCCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCGTTTGTTTCTAACAAACGttt                                                                                                                                        <  2:896539/140‑1 (MQ=255)
    tAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTtat                                                                                                                                      <  2:195166/141‑1 (MQ=255)
      aGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTGATTACCTACCACCAGCTTTAAATCGTTTGTTTCTAACAAGCGTTTATAc                                                                                                                                    >  1:402075/1‑141 (MQ=255)
      aGGGAAGATTTACCAGCACCATTCGGGCCCATGATAACAATTATTTCGCCGCGTATTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTTTTTCTAACAAGCGTTTATAc                                                                                                                                    >  1:260526/1‑141 (MQ=255)
       gggAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATAcc                                                                                                                                   >  2:1045623/1‑141 (MQ=255)
         gAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTACAAACAGATTTACATCTTTTGTTTCTAACAAGCGTTTATAcctc                                                                                                                                 >  2:855037/1‑141 (MQ=255)
          gaGATTTACCAGCACCAGTCGGGCCCATGATAACAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATAcctcc                                                                                                                                <  2:519852/140‑1 (MQ=255)
          aaGATTTCCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATAcctcc                                                                                                                                <  2:1054746/141‑1 (MQ=255)
          aaGATCTACCAGCACCATCCGGGCCCATGATACTAATTATTTCGCCGCGTACTAGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATAcctcc                                                                                                                                <  2:582497/141‑1 (MQ=255)
              tttACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAAt                                                                                                                            >  1:543222/1‑141 (MQ=255)
                tACCAGCACCATTCGGGCCCATGATACCTATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACACATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTa                                                                                                                          <  1:601205/141‑1 (MQ=255)
                  ccAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTGCATGCTTATTACCTAAAGACAGATTTAACTCTTTTGTTTCTACCAAGCGTGTATACCGCCTGATTaaa                                                                                                                        >  2:424909/1‑141 (MQ=255)
                  ccAGCACCATCCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGGGTCGCTAAGGAAATGTTTTTAAGTCCACCATTACTCCCTTGAAACTAATATCAATCTTTTGTTTCTAACAAGCGTCTATACCCCCTAATaaaa                                                                                                                        >  2:1017824/1‑141 (MQ=255)
                     gCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCAATAATGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGt                                                                                                                     >  1:35270/1‑141 (MQ=255)
                        ccATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTa                                                                                                                  <  2:677490/141‑1 (MQ=255)
                          aTTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAgg                                                                                                                <  1:857468/141‑1 (MQ=255)
                            tCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCt                                                                                                              >  1:860050/1‑141 (MQ=255)
                            tCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCt                                                                                                              >  1:22087/1‑141 (MQ=255)
                            tCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTCTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCt                                                                                                              <  1:757877/141‑1 (MQ=255)
                              gggCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAAACAAGATAAATCTTTTGTTTCAAACAAGGGTTTATACCTCCTAATTAAAAGTTTAGGCTaa                                                                                                            >  1:1041106/1‑141 (MQ=255)
                                 cccATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCt                                                                                                         <  2:813206/141‑1 (MQ=255)
                                  ccATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGATTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCta                                                                                                        >  1:827942/1‑141 (MQ=255)
                                     tGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaatt                                                                                                     >  2:253189/1‑141 (MQ=255)
                                        aaCCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaattaat                                                                                                  <  2:643879/140‑1 (MQ=255)
                                          ccAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTg                                                                                                <  1:1043818/141‑1 (MQ=255)
                                           cAATTACTTCGCCGCGAACTGGTACCCATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGt                                                                                               <  1:974/141‑1 (MQ=255)
                                            ccctgtttCGCCCCGTACTGGTACCGATAAGGAAATGTTGTGAAGTATATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTCGGCTAACTTAATTAATTGta                                                                                              <  2:465426/136‑1 (MQ=255)
                                            cattattTCGCCACGTACTGGTATCTATAAGCAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGta                                                                                              <  2:625660/140‑1 (MQ=255)
                                            aaTTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGta                                                                                              <  1:964218/141‑1 (MQ=255)
                                             attattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtat                                                                                             >  1:314635/1‑141 (MQ=255)
                                               tattTCGCCGCGTACTGGTAACGATAAGGAAATTATTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAAAAAGCGTTTATACATCCTAAATAAAAGTTTAGACCACACCAATTAATGGCataa                                                                                           >  2:246633/1‑141 (MQ=255)
                                                    cgccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTATATATTaaaa                                                                                      >  2:850834/1‑140 (MQ=255)
                                                    agccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATTAAc                                                                                      >  2:126283/2‑141 (MQ=255)
                                                        gcggtcTGGGATCGATAAGGAAATGTGTTTAAGGACGTGCGTATTACCTAAAAACAGATTTAAATCTTTTGGTTCTAACAAACGGTTATACCTCCTAATTAAAAGTTTAGGCTAACCTGATTAATTGTATAATAAACTgag                                                                                  <  1:476756/136‑1 (MQ=255)
                                                        gcatACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAATAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTgag                                                                                  <  2:262779/138‑1 (MQ=255)
                                                         cgTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTgaga                                                                                 >  1:184954/1‑141 (MQ=255)
                                                             cTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAatat                                                                             >  1:818593/1‑141 (MQ=255)
                                                                 tATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTAt                                                                         <  1:893836/141‑1 (MQ=255)
                                                                 tATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTAt                                                                         <  1:391066/141‑1 (MQ=255)
                                                                 tATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTAt                                                                         <  1:710453/141‑1 (MQ=255)
                                                                    tgATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCAt                                                                      <  1:416763/140‑1 (MQ=255)
                                                                    ctaTAAGGAAATTTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCAt                                                                      <  2:30240/139‑1 (MQ=255)
                                                                      aTAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGt                                                                    >  1:830114/1‑141 (MQ=255)
                                                                       tAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTACTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTAGAATAAACTGAGAATATTTATCATGTc                                                                   >  1:640937/1‑141 (MQ=255)
                                                                         aGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATTTCaa                                                                 >  2:66915/1‑141 (MQ=255)
                                                                          ggAAATGTTTTTACGTACATGCTTATTACCTAATATCAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATGAATTGTATATTATACTGAGATTATTTATCATGTCAAg                                                                <  2:848606/141‑1 (MQ=255)
                                                                          ggAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                                                                <  2:666999/141‑1 (MQ=255)
                                                                          ggAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCCTATGATTCAACAAAGAGATTAAACCTCCTAAGTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                                                                <  1:41157/141‑1 (MQ=255)
                                                                           gAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGTGAATATTTATCATGTCAAGt                                                               <  1:469706/141‑1 (MQ=255)
                                                                           gAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGt                                                               <  1:790527/141‑1 (MQ=255)
                                                                            aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAGTTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                                                              <  2:891795/141‑1 (MQ=255)
                                                                            aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                                                              <  2:384489/141‑1 (MQ=255)
                                                                              aTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                                                            >  2:193993/1‑141 (MQ=255)
                                                                                   tttttttttttttttattaCCTAAAAACAGATTTAAATCTTTTGTTTTTATCAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTATTTGTTTAATTAACTGAGAATATTTATATTTTTAAGTGAATTTGt                                                       >  2:935885/14‑141 (MQ=255)
                                                                                   tttttttttttttttattaCATAAAAACAGATTTAAATCTTTTGTTTCTAACAAGGGTTTATACCTCCTATTTTAAAGTTTAGGCTAACCTTTTTTATTTTTTTATTTACTGATAATATTTATTATGTTCATTAAATTTGt                                                       >  2:946467/14‑141 (MQ=255)
                                                                                   tttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCgg                                                       >  2:852050/1‑140 (MQ=255)
                                                                                        gTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatat                                                  <  2:416895/141‑1 (MQ=255)
                                                                                        gTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatat                                                  >  1:87933/1‑141 (MQ=255)
                                                                                         tACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatata                                                 <  1:385508/141‑1 (MQ=255)
                                                                                         tACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatata                                                 >  1:138373/1‑141 (MQ=255)
                                                                                         tACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatata                                                 <  1:82765/141‑1 (MQ=255)
                                                                                         tACATGCGTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatata                                                 <  1:130372/141‑1 (MQ=255)
                                                                                            aTGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataata                                              >  1:126822/1‑141 (MQ=255)
                                                                                                ttattaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGCTATAATATAGa                                          >  1:1055550/1‑141 (MQ=255)
                                                                                                ttattaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGa                                          >  2:351681/1‑141 (MQ=255)
                                                                                                     aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatg                                     >  1:995261/1‑141 (MQ=255)
                                                                                                       cTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgta                                   >  1:931424/1‑141 (MQ=255)
                                                                                                       cTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgta                                   >  2:1029721/1‑141 (MQ=255)
                                                                                                       cTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgta                                   >  1:982430/1‑141 (MQ=255)
                                                                                                       cTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgta                                   >  1:982321/1‑141 (MQ=255)
                                                                                                       cTAAAAACAGATTTAAATCTTATGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgta                                   <  2:622567/141‑1 (MQ=255)
                                                                                                          aaaaCGGATTTGAATCTTTTGTCGTTACCAAGCTTTTATACCCACCAATGAAAAGTTTAGGCTAACCTAACTAATTGTATAATAAACTGAGAATATATAGCATGTCAAGTAAATTCGTGATATAATATAGACAatgtatgt                                <  2:889959/141‑1 (MQ=255)
                                                                                                            aaCTGATTTAAATCTTTTGCTTCTAGCAAGCGTTTATACGTCCTAATTAAAAGTTTACGCTAACCTAATTAATTGTATAATAAACTGAGAATATGTATCACGATAACTTAATTCGTGTTATAATATAGCCAATGTATGTGa                              <  2:6153/141‑1 (MQ=255)
                                                                                                              cAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATCAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAgg                            >  1:112002/1‑141 (MQ=255)
                                                                                                                gATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCGAGTACATTCGTGATATACTATAGCCAATGTATGGGAgggg                          >  2:35599/1‑139 (MQ=255)
                                                                                                                 aTTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCATAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAGTTCGTGATATAATATAGACAATGTATAGGGGGTGa                         >  1:274054/1‑141 (MQ=255)
                                                                                                                    tAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGTCAATGTATGTGAGGTGAAAg                      <  1:641744/141‑1 (MQ=255)
                                                                                                                    tAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATAATGTCAAGTAAAATCGTGATAAAATATAGACACTGTATGTGAGGTGAAAg                      >  1:8248/1‑141 (MQ=255)
                                                                                                                    tAAATCTTTCGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAg                      >  2:823084/1‑141 (MQ=255)
                                                                                                                    tAAATCTTTAGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAg                      <  1:817784/141‑1 (MQ=255)
                                                                                                                     aaaTCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGt                     >  2:869767/1‑141 (MQ=255)
                                                                                                                        tCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATg                  <  1:683385/141‑1 (MQ=255)
                                                                                                                           tttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTa               >  1:1060646/1‑141 (MQ=255)
                                                                                                                           tttGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTa               >  1:698414/1‑141 (MQ=255)
                                                                                                                             tGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGCGGGGAAAGAATGTTAAc             >  2:596883/1‑141 (MQ=255)
                                                                                                                              ttttCTAACAAGTGTTTATACCTCCTAATTATAAGTTTAGGCTAACTTAATTAAGTGTATAATAAACTGAGAATAGTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACt            <  2:986599/140‑1 (MQ=255)
                                                                                                                              gTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACt            >  1:1030357/1‑141 (MQ=255)
                                                                                                                                ttCTAACAAGCGTTTATGCCTCCTAATTAAAAGTTTAGGGTAACCTAATTAATTGTATAATAAACTGAGAATATTTCTCATGACAAGTCGCTTCGTGCTATAATATAGACAATTTATGTTAGGTTAAAGTATGTTAACTga          <  2:469571/141‑1 (MQ=255)
                                                                                                                                tccTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGTCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTga          <  2:405999/139‑1 (MQ=255)
                                                                                                                                 tCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGAAAATGTATGTGAGGTGAAAGTTTGTTAACTgag         >  2:428085/1‑140 (MQ=255)
                                                                                                                                  cTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTgaaa        >  1:528487/1‑140 (MQ=255)
                                                                                                                                   tAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTgaaga       <  1:757586/141‑1 (MQ=255)
                                                                                                                                   tAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTgaaga       <  2:127825/141‑1 (MQ=255)
                                                                                                                                    aaCAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTgaagaa      >  1:887727/1‑141 (MQ=255)
                                                                                                                                    aaCAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTgaagaa      >  1:817055/1‑141 (MQ=255)
                                                                                                                                    aaCAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAAAGAATGTGAGGTGAAAGTATGTTAACTgaagaa      >  1:346681/1‑141 (MQ=255)
                                                                                                                                     aCAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGaaa     >  1:279826/1‑141 (MQ=255)
                                                                                                                                        taCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAag  <  1:841527/140‑1 (MQ=255)
                                                                                                                                        taCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAag  <  1:378167/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
TTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAG  >  CP000730/707773‑708049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: