Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,858 T→A 41.7% intergenic (+89/+19) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8580TA41.7% 135.0 / 134.3 117intergenic (+89/+19)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base T (42/25);  new base A (22/26);  total (64/53)
Fisher's exact test for biased strand distribution p-value = 8.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

TTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCT  >  CP000730/35725‑35992
                                                                                                                                     |                                                                                                                                      
tgtAATGGTGAAGCTATTGAGTATAAAGGTTTTGGCGAAAAATGATTACTTGAACAAGGGATAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGGAAAGGAAAAAAATATAGCTAACCACTAATTTATCATg                                                                                                                                 <  2:532993/139‑1 (MQ=37)
       tAGAAGCTATTCAGTATAAAGTTTTTTTTTTTTTATGATTACTTGAACAAGAGATAAAATAAAATTGTGACCTAATAAAAATAGAAGGTGCTGTTTCAAAGTAAAAAAATATAGCTAACCACTAATTTAGAATGTTAgttt                                                                                                                          >  2:954048/1‑139 (MQ=25)
       tAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtgt                                                                                                                          <  1:38360/141‑1 (MQ=255)
         gAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTc                                                                                                                        >  1:251378/1‑141 (MQ=255)
         gAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTc                                                                                                                        >  2:807098/1‑141 (MQ=255)
         gAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAACACATCATGCTTTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTc                                                                                                                        >  1:503491/1‑141 (MQ=37)
             cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                                                                    <  1:728697/141‑1 (MQ=255)
               aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTaa                                                                                                                  <  1:575768/141‑1 (MQ=255)
               aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTaa                                                                                                                  >  2:863764/1‑141 (MQ=255)
               aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCATaa                                                                                                                  >  1:340785/1‑141 (MQ=255)
                  cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCACTTAACtt                                                                                                               >  2:307110/1‑141 (MQ=255)
                   aGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTg                                                                                                              >  1:481578/1‑141 (MQ=255)
                     tataAAGTTTTTGGTGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                                                            <  2:701441/141‑1 (MQ=255)
                     tataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTCCt                                                                                                            >  2:864405/1‑141 (MQ=255)
                     aataAAGTTTTTAGCGAAAAAGGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAATAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                                                            <  1:981310/140‑1 (MQ=255)
                       taAAGTTTTTGGCGAAAAAAGATTACTTGAACAAGAGCGAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAg                                                                                                          >  1:592934/1‑141 (MQ=255)
                         aaGTTTTTGGCGAAAAATGATTATGGGAACAAGAGCGAAAATAAGATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAACAAATATGGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                                                        >  2:228692/1‑141 (MQ=21)
                         aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                                                        >  2:302298/1‑141 (MQ=255)
                         aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                                                        >  1:535387/1‑141 (MQ=255)
                            tttttGGCGAAAAATGATTACTTGAACAAGAGCTCAAACAAAAGTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatga                                                                                                     >  1:1048009/1‑141 (MQ=255)
                              tttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATCGCTAACCACTAATTTATCATGTCAGTGCTCGCTTAACTTGCTAGCatgatg                                                                                                   <  2:418202/141‑1 (MQ=255)
                                tGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCt                                                                                                 <  1:612994/141‑1 (MQ=255)
                                tGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGGTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCt                                                                                                 >  1:613353/1‑141 (MQ=255)
                                 ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCCTGTCAGTGTACGCTTAACTTGCTAGCATGATGCTa                                                                                                >  2:1011866/1‑141 (MQ=255)
                                 ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTa                                                                                                >  1:784829/1‑141 (MQ=255)
                                             ttACTTGCACAAGAGCTAAATTAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGAAAAAAAATATAGCTAACCTCTAATTTATCATGTCAGTGTTCGATTAACTTGCTTGCATGATGCTAATTTCGTGGCAt                                                                                    >  1:669136/1‑141 (MQ=18)
                                             ttACATGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAAGATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCAt                                                                                    >  2:733932/1‑141 (MQ=255)
                                                          agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATGGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                                                       >  1:649288/1‑141 (MQ=255)
                                                            cTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTCACTTGCTAGCCTGATGCTAATTTCGTGGCATGGCGAAAGCCCGTAg                                                                     >  2:131953/1‑141 (MQ=255)
                                                             taaaataaaaTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCCCTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGa                                                                    >  1:18956/1‑141 (MQ=255)
                                                               aaataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATc                                                                  >  2:820408/1‑141 (MQ=255)
                                                                aataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCt                                                                 >  1:630667/1‑141 (MQ=255)
                                                                 acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATGACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                              >  2:711322/15‑141 (MQ=11)
                                                                 acaaaagagtattttaataaAAATGATGGTTATGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                              >  2:886357/15‑141 (MQ=11)
                                                                 acaaaagagtattttaataaAAATGATGGGTCTGTTGCAAAGTAATAAATATAGCTAATAACTAATATATCATGTCAGTATTCGTTTAAATTGCTAGCATGATGCTAATTTCATGGCATGGGGCAAATCCGCAGATCTGaa                                                              >  2:514646/15‑141 (MQ=2)
                                                                   aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                            <  2:855521/129‑1 (MQ=11)
                                                                   aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                            <  1:679751/129‑1 (MQ=11)
                                                                   aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                            <  1:878693/129‑1 (MQ=11)
                                                                    aaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCATAGATCTggaa                                                             >  1:891641/1‑138 (MQ=255)
                                                                     aatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                            >  2:1044580/1‑141 (MQ=255)
                                                                     aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                                                          <  2:20577/131‑1 (MQ=11)
                                                                      agattattCTAATAAAAATGACGCTTCTGTTGTAAAGTAAAAAATATAGCTAATCACCAATATATCCTGTCAGTGTGCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCCGAAgtgaa                                                         <  2:180925/134‑2 (MQ=11)
                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTCATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                         <  2:936461/132‑1 (MQ=11)
                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                         <  1:359737/132‑1 (MQ=11)
                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTCTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                         <  2:408003/132‑1 (MQ=11)
                                                                       gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                        >  1:76510/9‑141 (MQ=11)
                                                                        tgtgATCTAATAAAACTAGAAGGTTCTGTTGCAAAGTAAAGAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGGTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                         <  1:929180/141‑1 (MQ=255)
                                                                        agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTCATCACTAATATCTCATGTCATTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                       <  2:377173/134‑1 (MQ=11)
                                                                         gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                        <  1:946563/141‑1 (MQ=255)
                                                                         gtattttaataaAAATGATGGCTCTGTTGCAAAGTAAAAAATATAGCTATTCACGAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                      <  1:141559/135‑1 (MQ=11)
                                                                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATATCTAATTACTAATATTTTTTGTTTGTTTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                     >  2:706637/6‑141 (MQ=11)
                                                                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                     <  1:1049656/136‑1 (MQ=11)
                                                                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                     <  2:915771/136‑1 (MQ=11)
                                                                           gagcTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCCGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                      <  2:339580/138‑1 (MQ=255)
                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCTCCg                                                    >  2:427998/5‑141 (MQ=11)
                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                    >  1:752790/5‑141 (MQ=11)
                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                    >  2:842339/5‑141 (MQ=11)
                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                    <  1:809754/137‑1 (MQ=11)
                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                    <  1:317039/137‑1 (MQ=11)
                                                                            ttttaataaAAATGTTGGTTCTCTTGCAAAGTAAAAAATTTAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   <  2:93710/138‑1 (MQ=11)
                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTGATATATCACGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   <  2:40379/138‑1 (MQ=11)
                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   >  2:661955/4‑141 (MQ=11)
                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   <  2:1049545/138‑1 (MQ=11)
                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   >  1:24355/4‑141 (MQ=11)
                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTCAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   >  2:1037636/4‑141 (MQ=11)
                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAAGATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   >  1:559041/4‑141 (MQ=11)
                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATAGAACTGATCGCTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   >  1:250794/4‑141 (MQ=11)
                                                                            ttttaataaAAAGGATGGTTCTGATGCAACATAAAAAATATAGCCAAGCACTAATCTATCATGTCAGTGTTCGTTTAACTTGCTAGCACGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   <  1:298438/138‑1 (MQ=11)
                                                                            aTCTAATAAAAATCGAAGGTTCTTGTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                     <  1:286100/141‑1 (MQ=255)
                                                                             tttaataaAAATGATGGTTCTGTTGTAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                  <  1:128496/139‑1 (MQ=11)
                                                                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                  <  2:807554/139‑1 (MQ=11)
                                                                             ttcaataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                    <  2:205616/138‑1 (MQ=255)
                                                                              ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATTGCTAATCGCTAATATATCATGTCAGTGTTCGTTTAACTTGCTCGCATGATGCTAATTTCGTGGCATGGCGAAGATCTGTAGCTCTGTAGGGACCTGCGGtt                                                 >  1:63753/2‑141 (MQ=11)
                                                                              tcatattaaaTAGCTGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCCGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATTGGGAAAATCCGTAGATCTGAAGAGAACTACGGt                                                  >  2:326890/8‑141 (MQ=11)
                                                                              taaaaaaataTAAAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   <  2:634667/136‑1 (MQ=12)
                                                                              cTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   >  1:653939/1‑141 (MQ=40)
                                                                              cTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGAgg                                                   >  1:339667/1‑141 (MQ=255)
                                                                              cTAATAAAAATAGAAGGTTCTGTAGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTAGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   >  2:759524/1‑141 (MQ=255)
                                                                               taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGGTCTGAAGAGACCTGCGGTTc                                                >  2:296800/1‑141 (MQ=11)
                                                                               taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAGGAGACCTGCGGttt                                                >  1:223600/1‑140 (MQ=11)
                                                                               taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                <  1:362143/141‑1 (MQ=11)
                                                                               taataaAAATGATGGATCTGTTGCAAAGTAAAAATGATAGCTCATCACTAATATATCATGTCAGCGTACGTTGAGCTTGCTAGCATGATGATAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                >  1:792935/1‑141 (MQ=11)
                                                                                aataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGAATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                 >  2:405487/1‑141 (MQ=255)
                                                                                aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtttt                                               >  1:888645/1‑139 (MQ=11)
                                                                                aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                               >  1:43771/1‑141 (MQ=11)
                                                                                  taaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATGTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                               <  1:411365/141‑1 (MQ=38)
                                                                                  taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                             <  1:488396/141‑1 (MQ=11)
                                                                                  aaaaaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                               <  2:370507/140‑1 (MQ=37)
                                                                                   aaaaatgtgtgggtctcTTGCAAAGTAAAAAATTTAGCTAATCACTACTCTCTCATGTCAGTGTTCGTTTAACTTGCTCGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAACAGACCTGCGGTTCttt                                             <  2:208488/128‑1 (MQ=1)
                                                                                    aaaaTAGAAGGTCCTGTTGCAAAGTAAAAAAATATAGCTAACCCGTAATTTATCATGTCAGTGTTCGCTTAACTTTCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                             <  2:545003/141‑1 (MQ=255)
                                                                                     caaatgctggctCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt                                            >  2:517100/12‑141 (MQ=0)
                                                                                     aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt                                            >  2:496871/1‑141 (MQ=35)
                                                                                        tAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat                                         <  1:98650/141‑1 (MQ=32)
                                                                                         aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                        <  2:75944/141‑1 (MQ=32)
                                                                                          ggaagtacTGTTTCAGAGTAATAAGTATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata                                      >  2:888788/8‑141 (MQ=1)
                                                                                          gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatat                                       >  2:963771/1‑141 (MQ=31)
                                                                                          gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatat                                       >  2:213725/1‑141 (MQ=31)
                                                                                           aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata                                      >  1:650425/1‑141 (MQ=11)
                                                                                                                           aatcACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTa      <  2:699055/138‑1 (MQ=0)
                                                                                                                               accaaTTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCt  <  1:579164/138‑1 (MQ=1)
                                                                                                                                     |                                                                                                                                      
TTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCT  >  CP000730/35725‑35992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: