Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 629,681 C→A 71.2% L84I (CTA→ATA)  USA300HOU_0564 → possible 3‑hexulose‑6‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730629,6810CA71.2% 79.2 / 47.8 67L84I (CTA→ATA) USA300HOU_0564possible 3‑hexulose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (11/8);  new base A (20/27);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 2.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

CGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGA  >  CP000730/629545‑629817
                                                                                                                                        |                                                                                                                                        
cGAAGGTTTAACATCATTGAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAgg                                                                                                                                      <  1:56159/141‑1 (MQ=255)
          ccAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCagaa                                                                                                                            <  1:522570/141‑1 (MQ=255)
            agcagTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCagaaga                                                                                                                          <  1:257826/141‑1 (MQ=255)
                    aaaatatGGCAAACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTTGCGCGGATGTAATTACAATACTAGTTGTTGCAGAAGACGCATCaa                                                                                                                  <  1:665057/138‑1 (MQ=255)
                    aaCATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCaa                                                                                                                  <  2:408197/141‑1 (MQ=255)
                    aaCATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCaa                                                                                                                  >  2:803988/1‑141 (MQ=255)
                         agggCAAAAAACATTAATAATGAAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTaaa                                                                                                             <  2:599955/139‑1 (MQ=255)
                           ggCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAagc                                                                                                           <  2:882566/141‑1 (MQ=255)
                           ggCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAagc                                                                                                           <  1:948485/141‑1 (MQ=255)
                           ggCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAagc                                                                                                           <  2:190488/141‑1 (MQ=255)
                           ggCAGACAAAATTAGGAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAagc                                                                                                           <  1:153396/141‑1 (MQ=255)
                             cAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAagcag                                                                                                         >  1:528978/1‑141 (MQ=255)
                               gACAACATGAGTAATGTAAAAGTATAAGCAGACATGAAAATTATAGATGAAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCt                                                                                                       <  1:618178/141‑1 (MQ=255)
                                   acaTTAGTAATGTAAAAGTATTAACAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCAGATGTAATTACAATAATAGGTGTTGAAGAAGAGGCATCAAATAAaacaacaaatg                                                                                                   >  2:827793/1‑134 (MQ=255)
                                     aTTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTgaa                                                                                                 >  1:1061849/1‑141 (MQ=255)
                                     aTTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTgaa                                                                                                 <  2:255141/141‑1 (MQ=255)
                                      ttAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGGAAGTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTgaag                                                                                                >  1:993694/1‑141 (MQ=255)
                                          tAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGc                                                                                            <  2:308233/141‑1 (MQ=255)
                                          tAATGTAAAAGTATTAGCAAACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGc                                                                                            <  2:308530/141‑1 (MQ=255)
                                              gTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCAt                                                                                        >  1:35482/1‑141 (MQ=255)
                                               tAAAAGTATTAGCAGACATCAAAGTGTTAGATTCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAAGTAAAGCAGCTATTGAAGAAGCTCATa                                                                                       <  2:741053/141‑1 (MQ=255)
                                                  aaGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaa                                                                                    <  1:48759/141‑1 (MQ=255)
                                                  aaGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaa                                                                                    <  1:705664/141‑1 (MQ=255)
                                                  aaGTACTAGCAGACATGAAAATGATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaa                                                                                    <  1:135752/141‑1 (MQ=255)
                                                   aGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAACTTTGGCGCGGATGTAATTACAATAATAGATGTTGCAGAAGATGCATCAATTAAAGCAGCAATTGAAGCAGCTAATaaaaa                                                                                   >  2:118180/1‑141 (MQ=255)
                                                        tAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAataata                                                                              <  1:600846/141‑1 (MQ=255)
                                                        tAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCAAAGAAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAaatagaa                                                                              >  1:538710/1‑138 (MQ=255)
                                                         aGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAAtaataa                                                                             >  1:183545/1‑141 (MQ=255)
                                                           cAGACATAAAAAATAGAAATGCAGGTGAGTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAAc                                                                           <  2:264570/141‑1 (MQ=255)
                                                             gACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCCAGCAATTGAATGTGGCGCGGATGTGATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATCGAAGAAGCTCATAAAAATAATAAACaa                                                                         >  1:624929/1‑141 (MQ=255)
                                                               gaTGAAAATTATAGATGCGGCTGATTATGAAGTTAGCAAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAAtt                                                                       <  2:1036207/140‑1 (MQ=255)
                                                                  gAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGGTGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACt                                                                    >  2:221251/1‑141 (MQ=255)
                                                                          taGATGCAGTCGATTATGAAGTAAGCAAAGCAATTAAATTGGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAAAAATCAACAATGTCTAGTTGata                                                            <  2:535126/141‑1 (MQ=255)
                                                                          taGATGCAGATGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGata                                                            <  1:183518/141‑1 (MQ=255)
                                                                           aGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGatat                                                           >  1:941818/1‑141 (MQ=255)
                                                                            gATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATg                                                          <  1:554241/141‑1 (MQ=255)
                                                                            gATGAAGCTGAATATGAAGTTAGACAAGTAATTAAATGTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATg                                                          <  1:928012/141‑1 (MQ=255)
                                                                                cAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGTGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAAAAATTACTAGTTGATATGATTg                                                      >  2:165460/1‑141 (MQ=255)
                                                                                     gagtATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGtt                                                 <  2:873369/138‑1 (MQ=255)
                                                                                     gATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGtt                                                 >  1:910973/1‑141 (MQ=255)
                                                                                     aaTTATGAAGTTAGCCAAGCAATTAAAATTGGCGCGGAAGTAAGTACAATAATAGGTGGTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGtt                                                 <  2:599709/140‑1 (MQ=255)
                                                                                        tATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCaa                                              >  1:275565/1‑141 (MQ=255)
                                                                                        tATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCaa                                              >  1:275847/1‑141 (MQ=255)
                                                                                        tATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCaa                                              >  1:425420/1‑141 (MQ=255)
                                                                                         aTGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAg                                             >  1:1039244/1‑141 (MQ=255)
                                                                                          tGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGa                                            <  1:188038/141‑1 (MQ=255)
                                                                                              gatAGCCAAGCAAGTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTa                                        <  1:153867/139‑1 (MQ=255)
                                                                                                tAGGCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTAGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTTATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGa                                      <  2:131208/141‑1 (MQ=255)
                                                                                                tAGCCAGGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGa                                      <  2:876752/141‑1 (MQ=255)
                                                                                                 aGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGaa                                     <  1:226440/141‑1 (MQ=255)
                                                                                                  gCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGaaa                                    >  1:419976/1‑141 (MQ=255)
                                                                                                   ccAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGaaaa                                   >  1:542933/1‑141 (MQ=255)
                                                                                                   ccAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAAAAATTACTAGTTGATAGGATTGCTGTTCAAGATTTACaaaa                                   >  2:279578/1‑141 (MQ=255)
                                                                                                   ccAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGaaaa                                   >  1:424399/1‑141 (MQ=255)
                                                                                                    cAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGCTATGATTGCTGTTCAAGATTTAGaaaaa                                  >  1:804206/1‑141 (MQ=255)
                                                                                                       gCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGGTCATAAAAATAATAAACAATTACTAGTGGATATGATTGCTGTTGAAGATTTGAAAAAACga                               >  2:534102/1‑140 (MQ=255)
                                                                                                         aaTTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGc                             >  1:398778/1‑141 (MQ=255)
                                                                                                         aaTTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGc                             <  1:154709/141‑1 (MQ=255)
                                                                                                           ttAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCaa                           <  1:690688/141‑1 (MQ=255)
                                                                                                            tAAATTTGGCGCGGATGTACTTACGGCATTTTTTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCaaa                          >  1:254154/1‑141 (MQ=255)
                                                                                                            tAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCaaa                          >  2:892968/1‑141 (MQ=255)
                                                                                                              aaTTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAGGATGCCACAATTAAAGCAGCTATTGAGGAAGCTCATAAAAATAATAAACAATTACTAGTTGATAGGATTGCTGTGCAAGAATTAGAAAAACGTGCaaaaa                        >  1:483275/1‑140 (MQ=255)
                                                                                                              aaTTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCaaaaa                        >  1:497017/1‑140 (MQ=255)
                                                                                                                   ggCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTa                   >  1:948095/1‑141 (MQ=255)
                                                                                                                      gcgGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGAt                >  2:708891/1‑141 (MQ=255)
                                                                                                                        cgATGTAATTACAATACGAGGTTTTGCAGAAGATGCAACAATTAAAGCAGCGATTGAAGAAGCTCATAAAAATAAGAAACAATTACTAGTTGATATGATTGCTGATCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGa              <  1:262843/140‑1 (MQ=255)
                                                                                                                             tAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGGTGAAATgg         >  1:313269/1‑141 (MQ=255)
                                                                                                                                    aataatAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGa  <  1:174994/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
CGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGA  >  CP000730/629545‑629817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: