Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,322 G→A 53.5% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3220GA53.5% 91.2 / inf 649intergenic (+380/‑754)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (158/141);  new base A (192/154);  total (350/299)
Fisher's exact test for biased strand distribution p-value = 5.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

Only 100 of 661 total aligned reads displayed.

ATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATA  >  CP000730/76182‑76459
                                                                                                                                            |                                                                                                                                          
ataGTAAAATTTTAACAAAAAGGAGTCTTCTGAATGAACTATTGCAGATATAAACAATTTAACCAGGATGTTATCACTGGAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgg                                                                                                                                            <  1:261049/141‑1 (MQ=11)
    tataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGTCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAAc                                                                                                                                        <  1:761384/141‑1 (MQ=255)
        aTTTTACCAAAACGGAGTCTTCCGTATGAACTATTTCAGACTGAAACAATTTAACAAGGATGGTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATGTGAAATATTAAGGGAACgtgg                                                                                                                                    <  2:728970/141‑1 (MQ=11)
        aTTTTAACAAAAAGGAGTCTTCGGTATGGACTATTTCAGATACAAACAATGTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtgg                                                                                                                                    <  1:323260/141‑1 (MQ=11)
         ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACACTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggt                                                                                                                                   >  2:688189/1‑141 (MQ=17)
         ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggg                                                                                                                                   >  1:475091/1‑140 (MQ=17)
          tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggtg                                                                                                                                  >  1:1029571/1‑141 (MQ=32)
            tAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                                                                                                                                <  2:517505/141‑1 (MQ=17)
             aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaa                                                                                                                               >  2:432931/1‑141 (MQ=17)
             aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATATAGCATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                                                                               >  1:200925/1‑141 (MQ=11)
              aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaaa                                                                                                                              <  1:182398/141‑1 (MQ=17)
              aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaa                                                                                                                              >  2:933953/1‑141 (MQ=17)
                 aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                                                                           >  1:982666/1‑141 (MQ=12)
                    aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGGTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtca                                                                                                                        <  1:368340/141‑1 (MQ=255)
                     ggAGTCTTCTGTATGAACTAGTTCAGATAGAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcat                                                                                                                       <  2:622688/141‑1 (MQ=12)
                       ggTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatca                                                                                                                     <  2:863678/140‑1 (MQ=12)
                       aGTCTTGTGGATGAACTCTTTGAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatca                                                                                                                     <  2:499958/141‑1 (MQ=11)
                        gTCTTCTGTATGAACTATTTCAGATATAAACACTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGGTATCGTGATATGTCTGAAATATTACAGGAAGGTGGTGTAAACGTTcatcat                                                                                                                    >  1:92070/1‑141 (MQ=11)
                        gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTcatcat                                                                                                                    <  2:456299/141‑1 (MQ=17)
                         tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCAtt                                                                                                                   >  2:370139/1‑141 (MQ=17)
                             ctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGACATATTAAGACAACGTGGTGTAAACGTTCATCATTCAAc                                                                                                               >  2:879967/1‑141 (MQ=255)
                                 aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                                           <  1:607125/141‑1 (MQ=32)
                                  tcaacgctttCAGATAAAAACAATTTAACAAGGATGTTATCACTGTAGTCGTTGGCTACTAGCTACGATATGCATTGAGCCATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCt                                                                                                          <  1:442946/134‑1 (MQ=11)
                                  tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAGAGGTTGATGATTCAACGGTCt                                                                                                          >  1:136279/1‑141 (MQ=11)
                                   gAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTa                                                                                                         <  2:1005146/141‑1 (MQ=17)
                                    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                                                                                        <  2:894236/141‑1 (MQ=17)
                                    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATAGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                                                                                        <  1:353367/141‑1 (MQ=17)
                                    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCGACATGCAACGAACTAc                                                                                                        >  2:539144/1‑141 (MQ=11)
                                    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                                                                        >  2:114042/1‑141 (MQ=12)
                                    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                                                                        >  1:789669/1‑141 (MQ=255)
                                     aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                       <  1:1023442/141‑1 (MQ=12)
                                         tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTg                                                                                                   <  1:1002377/141‑1 (MQ=17)
                                          ttCAGATATAGACAATTTAACAAGGATGTTCTAACTGTAGCCGTTGACTACTTTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTgg                                                                                                  >  2:483910/1‑141 (MQ=11)
                                           tCAGATATAAACAATTTAAGAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                                                 <  2:198522/141‑1 (MQ=17)
                                           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                                                 <  1:425680/141‑1 (MQ=17)
                                           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                                                 <  2:945047/141‑1 (MQ=32)
                                            cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGt                                                                                                >  2:102390/1‑141 (MQ=12)
                                             aGATATAAACAATTTCACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGtt                                                                                               >  2:913293/1‑141 (MQ=17)
                                             aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                                               >  2:59142/1‑141 (MQ=12)
                                               atatAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCa                                                                                             >  2:721185/1‑141 (MQ=17)
                                               atatAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCa                                                                                             <  2:471420/141‑1 (MQ=12)
                                                tataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATGTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTATCGTTGTGTTCaa                                                                                            >  2:310443/1‑141 (MQ=255)
                                                tataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCTACGACCTACCGTTGGGTTCaa                                                                                            <  1:545585/141‑1 (MQ=11)
                                                 ttaAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                                                                           <  2:15177/140‑1 (MQ=17)
                                                      cAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                                                      >  2:981463/1‑141 (MQ=255)
                                                        aTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGc                                                                                    <  1:156016/141‑1 (MQ=17)
                                                         tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                                                   <  2:575929/141‑1 (MQ=12)
                                                         tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                                                   <  2:52945/141‑1 (MQ=12)
                                                           tAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAAAATGcccc                                                                                 >  2:44383/1‑141 (MQ=17)
                                                            aaCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCa                                                                                >  1:382617/1‑141 (MQ=17)
                                                             aCAAGGATGTTATCGCTGTCGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                               >  1:580572/1‑141 (MQ=11)
                                                             aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAGCGTGGTGTAAACTTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                               <  1:117088/141‑1 (MQ=12)
                                                             aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                               <  1:223318/141‑1 (MQ=17)
                                                             aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACTTTCATCATTCAAGGATGTACCGTTGGGTTCAAGAATATGCCCCat                                                                               >  1:241497/1‑140 (MQ=11)
                                                             aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                               >  1:1058508/1‑141 (MQ=255)
                                                               aaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtt                                                                             >  2:172529/1‑141 (MQ=255)
                                                               aaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtt                                                                             >  1:712207/1‑141 (MQ=12)
                                                               aaGGATGTTATCACTGTAGCCGTGGGCTACTATCTAAGATATGCATTGAGCTATCGGGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGCATATGCCCCaagt                                                                             >  2:552358/1‑139 (MQ=11)
                                                                  gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCTCAATTTta                                                                          >  1:537733/1‑141 (MQ=255)
                                                                     gTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                       <  1:883219/141‑1 (MQ=25)
                                                                     gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                       >  1:56955/1‑141 (MQ=34)
                                                                      ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                                                      >  2:1054305/1‑141 (MQ=255)
                                                                      ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                                                      >  1:673753/1‑141 (MQ=255)
                                                                       tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                     <  2:393301/141‑1 (MQ=255)
                                                                         tCACTGTAGCTGTTGGCTACTATCTCAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                                                   >  2:40421/1‑141 (MQ=21)
                                                                              gTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                              >  1:951353/1‑141 (MQ=25)
                                                                              gTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                              <  1:28192/141‑1 (MQ=25)
                                                                               tAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                                             <  2:330590/141‑1 (MQ=255)
                                                                                aGCCGTTGGCTACTATCTAAGATATGAAAAAGGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                            >  1:513563/1‑141 (MQ=11)
                                                                                aGCCGTTGGCTAATATCTAAGATATGCATTGAGCTATCGTGATATATCTCAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                            <  2:241411/141‑1 (MQ=11)
                                                                                 gctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                           >  2:799874/4‑141 (MQ=25)
                                                                                 gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                           >  2:1038910/1‑141 (MQ=255)
                                                                                 gCCGTTGGCTACTATCTAAGAGATGCATTGAGCTATCGTGATATATCTGAACTATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGCTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTGGgaag                                                           >  1:810922/1‑141 (MQ=11)
                                                                                  ctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAACTTTGgaaga                                                          >  2:339829/3‑141 (MQ=25)
                                                                                  ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                                                          >  2:757080/1‑141 (MQ=255)
                                                                                       ggCTACTATCCAAGATATGCGTTGAGCTATCGTGATATATCTGCAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAGTTTATATCAAATTTGGAAGAAAAAg                                                     <  2:962066/141‑1 (MQ=11)
                                                                                        gCTACTATCTAATATATGCATTGAGTTATCGGGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                                                    <  2:275119/141‑1 (MQ=21)
                                                                                         ctactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                  <  2:814285/141‑1 (MQ=35)
                                                                                          tactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                 <  1:931514/141‑1 (MQ=35)
                                                                                           actaTCTTAGATATGCATTGAGCTATGGTGATATATCTGAAAGATTCAGAGAACGTGGGGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                <  1:664068/141‑1 (MQ=11)
                                                                                           actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGCGAACGTGGTTTAATCGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                <  1:1040986/141‑1 (MQ=11)
                                                                                           actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                >  2:222694/1‑141 (MQ=255)
                                                                                           actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                >  1:523606/1‑141 (MQ=255)
                                                                                               tCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACGGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa                                            <  1:638263/141‑1 (MQ=25)
                                                                                               tCTAAGATATGCATCGAGCTATCGTGATATATCTTAAATATTAAGAGAACGTACTGTGCACTTGCATTATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa                                            <  1:503951/141‑1 (MQ=11)
                                                                                                ttAAGCTATGCATTGCTCTATCGTGATAGATCTGCAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATTTACCGTTGGATTCAAGAATATGCCCCAATTTTATATCAAATCTGGAAGAAAAAGCATaaaaaa                                           <  2:775665/140‑2 (MQ=11)
                                                                                                cTAAGATATGCATTGAGTTATCGTGATATATCTGAACTATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGACGAAAAAGCATaaaaaa                                           <  1:590020/141‑2 (MQ=21)
                                                                                                cTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGAATaaaaaa                                           >  1:850862/1‑140 (MQ=21)
                                                                                                 tAAGATATGCATTGAGTTATCGTGATATATTTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaag                                          >  2:287062/1‑139 (MQ=17)
                                                                                                  aaGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCt                                         >  2:60448/1‑141 (MQ=38)
                                                                                                   aGATATGCATTGAGTTATCGTGATATATCTGAAAGATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                        >  1:860364/1‑137 (MQ=17)
                                                                                                    aatatGCATTGAGTTATCGTGATATATATATTATATGAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGATAAAGCAGaaaaaagctt                                       <  2:1014436/140‑6 (MQ=11)
                                                                                                     atatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAAt                                      >  2:131478/1‑141 (MQ=40)
                                                                                                     atatGCATTGAGTTATCGTGATATATATGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATGTTAATACCAATTTGGAAGAAAAAGCATAAAAAGCTAAt                                      >  2:635765/1‑141 (MQ=14)
                                                                                                     atatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagctta                                      >  1:623494/1‑135 (MQ=11)
                                                                                                      tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATACGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAAtt                                     <  1:826477/141‑1 (MQ=255)
                                                                                                        tGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaaacttatta                                   >  2:262861/1‑132 (MQ=11)
                                                                                                                gTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCg                           <  1:407679/141‑1 (MQ=39)
                                                                                                                   aTCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAAAAAAAAGCTAATTACAAATGGCGTa                         >  2:850658/1‑141 (MQ=1)
                                                                                                                                         aaGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACACATGGCGTATTGATGAGACGTACATCAAAGTa  >  2:6602/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                          
ATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATA  >  CP000730/76182‑76459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: