BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,161	T→C	30.0%	I131V (ATT→GTT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,161	0	T	C	30.0%	787.9 / inf	786	I131V (ATT→GTT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (260/288); new base C (97/139); total (358/428)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 798 total aligned reads displayed.

CTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36021‑36301

ctttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATTGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTATCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAt                                                                                                                                              <  1:672795/141‑1 (MQ=255)

    cttACTTTAATATGACAGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTATGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAcc                                                                                                                                          >  1:630443/1‑141 (MQ=255)

     ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTCTAAGTTTAAACGATAGAAGAAGTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCt                                                                                                                                         >  1:994022/1‑141 (MQ=14)

       tcTTTAATATGACGGTGATCTTGCTCAATGATGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCCGTAATTACCttt                                                                                                                                       <  2:676438/140‑1 (MQ=11)

       aCTTTAATCTGACGCAGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                                                                                                       >  2:492380/1‑141 (MQ=11)

         tttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTg                                                                                                                                     <  1:18479/141‑1 (MQ=255)

         tttAATATGACGGGGATGTTGCTCAATGAGGTTATTCAGATATTTCGATGGACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGATGAAGGTGCCTGATCTGTAATTACCTTTTg                                                                                                                                     >  2:780933/1‑141 (MQ=11)

          ttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATGACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGa                                                                                                                                    <  1:461816/141‑1 (MQ=21)

          ttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTCTTTCAGCCCCTTCTGCCTGCGTTGAAGGTTGCTGATCTGTAACTACGTTTTgg                                                                                                                                    >  2:220351/1‑140 (MQ=11)

            aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACTATGACAGTCAGGATTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTCATCTGTTATTACCTTTTGAgg                                                                                                                                  <  2:727010/141‑1 (MQ=255)

            aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAgg                                                                                                                                  >  1:893278/1‑141 (MQ=25)

            aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAgg                                                                                                                                  >  2:187883/1‑141 (MQ=255)

              tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGtt                                                                                                                                <  1:258182/141‑1 (MQ=25)

              tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggct                                                                                                                                <  2:965744/141‑3 (MQ=255)

                tGATGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTa                                                                                                                              <  2:712808/141‑1 (MQ=11)

                tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGGAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTa                                                                                                                              >  1:86308/1‑141 (MQ=255)

                tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGGACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTa                                                                                                                              >  1:1000204/1‑141 (MQ=255)

                 gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTAGATGTACAATGGCAGACAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACTTCTTGAGGTTTAc                                                                                                                             >  2:267440/1‑141 (MQ=11)

                 gACGGTGATAATGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTCATCTGTAACTACCTTTTGAGGCTTAc                                                                                                                             >  1:778529/1‑141 (MQ=11)

                  ccGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                            <  2:43771/140‑1 (MQ=18)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAcc                                                                                                                            >  2:306944/1‑141 (MQ=255)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                            >  1:798618/1‑141 (MQ=35)

                    gttGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaa                                                                                                                          <  1:59642/139‑1 (MQ=11)

                    ggTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTGCTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGCGGTTTACCaa                                                                                                                          >  2:329191/1‑141 (MQ=255)

                    ggTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaa                                                                                                                          >  2:881896/1‑141 (MQ=18)

                    ggTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaa                                                                                                                          <  1:1031515/141‑1 (MQ=32)

                     gTGATCTTGCCCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGAGGCGATTTAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTCTGAGGTTTACCaaa                                                                                                                         <  1:555251/141‑1 (MQ=11)

                        aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGGTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                                                                                                      >  2:1020197/1‑141 (MQ=12)

                         tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTGGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                     >  1:713836/1‑141 (MQ=11)

                         tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                     <  2:613137/141‑1 (MQ=255)

                         tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                     >  1:336025/1‑141 (MQ=11)

                          cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATGGCTACCTTCGTTGAAGGTGCCTGATCTGTACTTACCTTTTGACGTTTACTAAATTGtt                                                                                                                    >  2:1058718/1‑141 (MQ=11)

                            tGCTCAATGATGTTATTCAGATATTTCGATTTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTa                                                                                                                  <  2:982069/141‑1 (MQ=11)

                                cAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                                              <  1:930591/141‑1 (MQ=12)

                                 aaTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                                             <  1:651010/141‑1 (MQ=17)

                                  aTGGGGTTATTTATAAATTTCGATTTACAATGACAGTAAGGTTTAGTTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATgaga                                                                                                            <  2:29044/141‑1 (MQ=11)

                                     aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                         <  1:613890/141‑1 (MQ=17)

                                          aTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGAt                                                                                                    >  1:538207/1‑141 (MQ=12)

                                           ttCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATa                                                                                                   <  1:106504/141‑1 (MQ=17)

                                              aGATATTTCGATGTACAATGACAGTCAGTTTTAAGTTTAAAAGCTTTAATTATTTTACCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATaatc                                                                                                <  2:455629/141‑3 (MQ=11)

                                               gATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                                               <  2:125584/141‑1 (MQ=17)

                                                atatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATATACGc                                                                                              <  2:642458/141‑1 (MQ=11)

                                                atatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGc                                                                                              >  2:1030220/1‑141 (MQ=32)

                                                 tatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                             <  1:299882/141‑1 (MQ=17)

                                                  atTTCGATGTACCATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                                            <  2:955381/141‑1 (MQ=12)

                                                    ttCGATGTACAATGGCAGTCAGGGTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTTAAGGTGCCTGATCTGTAATTCGCTTTTGAGGTTTACAAAATTGTTTAATGAGACGTTTGATAAATGCatat                                                                                          >  1:380744/1‑141 (MQ=11)

                                                     ttcaTGTACGATGGCAGGCATGTTTAAGTTTAAAAGCTTTACTTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                                                         <  2:943535/138‑1 (MQ=11)

                                                     tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGGGCCGTTTGGTAAACGCATATg                                                                                         >  1:447177/1‑141 (MQ=11)

                                                       gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGGTTGATAAACGCATATGCt                                                                                       >  1:434390/1‑141 (MQ=12)

                                                       gATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                                                       >  2:897751/1‑141 (MQ=255)

                                                        aTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATAAGACGTTTGATAAACGCATATGCTg                                                                                      >  1:199255/1‑141 (MQ=11)

                                                            aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTAATTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                  <  2:1035359/141‑1 (MQ=12)

                                                                             taggtgtaaAAGCGTTAATTAGTTTAGCTATTGCTGCCTTCGTTGAAGGTGCCGGTTCTGTAACTATCTTTTGAGGCTTACCAAAGTGTTTAATGAGACGTTTGATAAACGCATACGCTGAATGATTATCTCGTTGCTTAc                                                                 <  1:23371/135‑1 (MQ=1)

                                                                                  ttaagatctgttaTTGCTTTACCGAATGCTGCCTTGGTTGAAGGTGCCTGACCGGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGATACGTTTGATAGACGGATAGGCTGTATGATTGGCGCGTTGCTTACGCAAc                                                            <  2:741217/129‑1 (MQ=0)

                                                                                                   tttcccccttgttgccTTCGTTCAAGGTGCCTGATCTGTAACGCCCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTGTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                           <  1:359878/127‑1 (MQ=11)

                                                                                                   tttAACCATTGCTACTTTGTTTGAAGGTGCTTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                           <  1:740689/141‑1 (MQ=11)

                                                                                                     tAGCCATTGGTGCCTTCGTTGAAGGTGCCTTATCTGTAACTTCCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGTTAATCGCATATGCTGAATGATTATCTCGTTGCTTACGAAACCAAATATCTAATGTATGTc                                         <  1:281227/141‑1 (MQ=11)

                                                                                                      aaccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGCTTCTCTCGTTGCTTACGCAACCAACTATCTCATGTATGTcc                                        >  1:523081/3‑141 (MQ=255)

                                                                                                      aaccATTGCTACCCTCGTTGAAGGTGCCTTATCTGAAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                        <  2:416993/139‑1 (MQ=12)

                                                                                                      aGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCGAATGTATGTcc                                        >  1:967330/1‑141 (MQ=17)

                                                                                                       gCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTccc                                       <  2:654871/141‑1 (MQ=12)

                                                                                                       accATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTccc                                       >  1:127791/2‑141 (MQ=17)

                                                                                                       accATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTAAGCAACCAAATATCTAATGTATGTccc                                       >  2:924261/2‑141 (MQ=17)

                                                                                                        ccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGGGACGTTTGATAAACGCATATGTTGAATGATTATCTCGTTGCTTAGGCAACCAAATATCTTATGTATGTCCct                                      >  1:693089/1‑141 (MQ=255)

                                                                                                         cactgctgcCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                     <  1:214413/138‑1 (MQ=12)

                                                                                                         cATTGCTACCTCCGGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGCTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                     <  2:760882/141‑1 (MQ=12)

                                                                                                         cATTCCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCGAATTGTTTAATGAGACGTTTGATAAACGCATATGCGGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                     <  2:247888/141‑1 (MQ=12)

                                                                                                          aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCCTACGCAACCAAATATCTAATGTATGTCCccct                                    >  2:54976/1‑141 (MQ=17)

                                                                                                            tgcagcctccattGAAGGTTCCTGATCTGTAACAACCTATTAAGGCATACCAAATTGTTTAATGAGACATTTAATAAACGCATATGCTGAATGATTACCTCGTTGCCTACGCAACCAAAAATCTAATGTATGTCCCTCTgt                                  <  2:389390/130‑2 (MQ=11)

                                                                                                              cTACCTTCGTTGAAGGTGCCTGAACTGTAATTACCTTTTGAGGTTTACCAAGTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATATAATGTATGTACCTCTGTAt                                >  2:513563/1‑141 (MQ=11)

                                                                                                                tccTTCGTTGTAGGTGCCTGATCGGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTGGATAAACGTATATGCTGAATGATTATCTCGTTCCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                              <  2:691016/140‑1 (MQ=11)

                                                                                                                gccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                              <  2:860833/140‑1 (MQ=255)

                                                                                                                aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGGCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTTCCTCTGTATCa                              >  1:835772/1‑141 (MQ=12)

                                                                                                                aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGGTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                              <  1:878467/141‑1 (MQ=17)

                                                                                                                 ccatCGTTGAAGTTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             <  2:914430/138‑1 (MQ=11)

                                                                                                                 ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             >  1:1020192/1‑141 (MQ=17)

                                                                                                                 ccTTCGGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGTTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                             <  1:575341/141‑1 (MQ=17)

                                                                                                                  cTTCGTTGAAGGTGTCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                            <  2:204658/141‑1 (MQ=17)

                                                                                                                  cTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                            <  2:34337/141‑1 (MQ=17)

                                                                                                                  cTTCGTTGAAGGTGCCCGATCTGTAACTCCCTTTTGCGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                            <  1:502517/141‑1 (MQ=11)

                                                                                                                   ttCGTTTAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTCAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCTATg                           <  1:762716/141‑1 (MQ=11)

                                                                                                                   ttCGTTGAAGGTGCCTGATCTGTAATTACTTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                           <  2:847243/141‑1 (MQ=17)

                                                                                                                   ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGTCGTTTGATAAAAGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATg                           <  1:905087/141‑1 (MQ=17)

                                                                                                                    tCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAGATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATGTCTAATGTATGTCCCTTTGTATCAATgg                          >  2:567834/1‑141 (MQ=255)

                                                                                                                      gTTTAAGGTGTCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTCTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                        <  1:417208/141‑1 (MQ=255)

                                                                                                                      gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATggac                        >  2:149793/1‑139 (MQ=17)

                                                                                                                      gTTGAAGGTGCCTGATCTGTAACTACCTTTTGATGCTTACCAAATTGTTTAATGTGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGcc                        >  1:30920/1‑140 (MQ=11)

                                                                                                                            ggTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatat                  >  1:1055627/1‑141 (MQ=255)

                                                                                                                               gCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATTTaaa               >  1:851849/1‑141 (MQ=17)

                                                                                                                                 cTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTCTGTCCCTCTCTAGCAATTGTACGatattactc             >  2:75289/1‑136 (MQ=11)

                                                                                                                                 cTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATCAATa             >  1:473782/1‑141 (MQ=255)

                                                                                                                                    aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt          >  2:364051/1‑141 (MQ=17)

                                                                                                                                    aTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt          <  2:16760/141‑1 (MQ=12)

                                                                                                                                     tgtGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTc         <  2:595514/139‑1 (MQ=17)

                                                                                                                                      ttGTAACTACCTTTTGAGGTCTACCAAATTGTTTATTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc        <  1:166561/140‑1 (MQ=255)

                                                                                                                                      cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc        >  2:1048875/1‑141 (MQ=17)

                                                                                                                                      atGTAATGAACTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCACATATCTAACATATGTCCCTCTGCATCAATGGCACGATATAAATAGCTcc        <  1:149793/140‑1 (MQ=11)

                                                                                                                                         tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCCCGATATAAATAGCTCCttt     >  1:393484/1‑141 (MQ=12)

                                                                                                                                         tAATTACCTTTTGAGGTTTACAAAATTGTTTAATGAGACGTTTGATGAACGCATATGCTGAATGGTTATCTCGTAGGTTACGCAACCAAATATCTCACGTATGTCCGTCTGTAACAATGGCACGacatcagatgctacgtt     >  2:830307/1‑128 (MQ=11)

                                                                                                                                            ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTc  <  2:790833/141‑1 (MQ=32)

CTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36021‑36301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`