BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,997	T→C	29.0%	T185T (ACA→ACG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,997	0	T	C	29.0%	742.6 / inf	608	T185T (ACA→ACG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (222/206); new base C (95/81); total (318/290)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 611 total aligned reads displayed.

CAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTT  >  CP000730/35867‑36137

   tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                                                                                 >  1:281085/1‑141 (MQ=11)

   tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                                                                                 >  1:362747/1‑141 (MQ=17)

     ttcgtgtAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa                                                                                                                               <  2:38833/135‑1 (MQ=11)

       cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                                                                                                             >  1:123922/1‑141 (MQ=17)

        gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                                            >  2:394811/3‑141 (MQ=17)

        gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                                            >  2:122974/3‑141 (MQ=17)

        gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGGAGAGACCTGCGGTTCTTTTTATATAGAGCGTAGATACACTCAATACCTGTTAAAGTATTCTTTGCTGTCTTGTTACTTTgtttc                                                                                                                            >  1:383632/1‑137 (MQ=11)

         tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAAGACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                                                           >  2:270292/2‑141 (MQ=12)

         attAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTATATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAATTATTCTTTGCTGTATTGATACTTTGTTAcc                                                                                                                           <  2:240735/140‑1 (MQ=255)

          ttACCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                                                                          <  1:235114/141‑1 (MQ=17)

           tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                                                                         >  2:368203/1‑141 (MQ=17)

            aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                                                                                        <  2:65327/141‑1 (MQ=32)

              cTTGCTAGCATGATGCTAATTTCGTGGCCCGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtc                                                                                                                      >  2:3774/1‑141 (MQ=11)

              cTTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                                                      >  1:286412/1‑141 (MQ=17)

                    aGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttctt                                                                                                                >  1:322154/1‑141 (MQ=255)

                            gCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                                                                                        >  1:200802/1‑141 (MQ=255)

                            gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATCCTCTCATTCTTTTTCTTTCTTACTTTAat                                                                                                        <  2:280884/141‑1 (MQ=11)

                             cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                                                                                                       >  1:105638/1‑141 (MQ=25)

                             cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGATCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                                                                                                       >  1:391136/1‑141 (MQ=25)

                              tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                                                      >  1:100172/1‑141 (MQ=21)

                              tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                                                      >  1:263279/1‑141 (MQ=25)

                              tAATTGCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTTTTTGTATATAGAGCGTCAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAatat                                                                                                      <  1:150680/141‑1 (MQ=11)

                               aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGTGACTTTGATATCTTGTCTTTCTTACTTTAATATg                                                                                                     >  2:201052/1‑141 (MQ=11)

                               aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                                                     >  1:340253/1‑141 (MQ=21)

                                aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGa                                                                                                    >  1:168288/1‑141 (MQ=25)

                                   tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTTTATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                                                                                 >  2:291870/1‑141 (MQ=255)

                                   tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                                                                                 >  1:85802/1‑141 (MQ=35)

                                   tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                                                                                 <  2:361765/141‑1 (MQ=38)

                                    cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                                                <  1:333866/141‑1 (MQ=38)

                                     gTGGCATGGCGAAAATCCGTATATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATGCTTTGATACCTTGTCTTTTTTGCTTTAATATGACGGTg                                                                                               <  1:220421/141‑1 (MQ=14)

                                      tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                                              >  2:316275/1‑141 (MQ=37)

                                        gCATTGTGAAAATCCGTAGATCTGAACATACTTGCGGTTCTTTTTATATAGAGCGTTAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTCCTTTAATATGACGGTGATc                                                                                            <  2:195498/141‑1 (MQ=14)

                                            ggggAAAATCCGTAGATCTGAAGAGACCTACGTCTTTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                                                        <  2:143963/138‑1 (MQ=11)

                                             gCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                                                       <  2:340253/141‑1 (MQ=255)

                                                  aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGCGCGTAAATACAGTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                                                  <  2:217965/141‑1 (MQ=14)

                                                  aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAAGACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                                                  <  1:171117/141‑1 (MQ=255)

                                                  aaTCCGTAGATCTGAAGAGACCTACGGTACTTTTTATATAGAGCGTAAATACATGCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATTTTTTGTCTTTTTTACTTTAATTTGACGGTGTTCTTGTTCATTg                                                                                  >  1:255695/1‑141 (MQ=11)

                                                    tCCGTAGATCTTAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                                                                                <  2:46131/141‑1 (MQ=255)

                                                    tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                                                                                <  1:312581/141‑1 (MQ=255)

                                                    tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAAGACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTCATCTTGCTCAATGAg                                                                                <  1:291836/141‑1 (MQ=255)

                                                     ccGTCGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                               >  2:298574/1‑141 (MQ=255)

                                                     ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAGTTTGATACCTTGTCTTTCTTACTGTAATATGACGGTGATCTTGCTCAATGAgg                                                                               >  1:34815/1‑141 (MQ=255)

                                                     ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                               <  1:352001/141‑1 (MQ=255)

                                                     ccGTAGATCTGAAGAGACCTGCGGTCCCAATTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                               >  1:191545/1‑141 (MQ=11)

                                                     ccGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                               >  1:376946/1‑141 (MQ=255)

                                                     ccGTACATCTGAAGAGACCTTGGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                               <  1:41872/141‑1 (MQ=255)

                                                           aTCTGAATAGACCTGCGGTTCTTTTTATATCGTGCCTAAATATTCGCATTACCTTTTAACATATTATTAGCTGGATTGATCCTTTGATACCTTGTCTTTCTTACTTTGAAATAACGGTTATTTTGCTCATTTATGTTATTc                                                                         <  2:52216/141‑1 (MQ=2)

                                                             cTGAAGAGACCTGCGGTTCTTTTTCTATAGGGCGTAAATGCCCTCGATTCCGTTTAGCGTCGTGTTTGGGTTATTTATACTTTGATACCTTGTCTTTCTTTCTTTAGTATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                                                       <  2:34393/141‑1 (MQ=2)

                                                             cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTcgg                                                                       >  1:116358/1‑139 (MQ=255)

                                                             cTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                                                       <  2:127596/141‑1 (MQ=255)

                                                             cTGAAGAGACCTACGGTTCTATTTATATAGAGCGTAAATACATTGAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGCTGATCTTGCTCAATGAGGTTATTCAg                                                                       >  1:240718/1‑141 (MQ=11)

                                                               gAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat                                                                     >  1:187333/1‑141 (MQ=255)

                                                                  gagaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                                                  <  1:327422/141‑1 (MQ=255)

                                                                   agagcgacGGTTCTGTTTAGATAGGGCGTAAATGCATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGCTATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt                                                                 <  2:150181/134‑1 (MQ=11)

                                                                   agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTCAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt                                                                 >  1:250495/1‑141 (MQ=255)

                                                                   agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATCCAGATAttt                                                                 >  2:135979/1‑141 (MQ=255)

                                                                     aCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg                                                               <  1:395758/141‑1 (MQ=255)

                                                                        tacGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAACTCTTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGGTCTTGCTAAATGAGGTTATTTAGATATTTCTAtt                                                            >  2:230079/3‑140 (MQ=11)

                                                                         gCGGTTCTTTTTATATAGAGCGTACATACACTAAATACCTTTTAAAGCATTCTTTGCTGTATTGATACTTTGATACTTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                           <  2:115834/141‑1 (MQ=11)

                                                                         gCGGTTCTTTTTATATAGAGCGTAAATAACATCCATAGCATGTTTAGGATGCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGATGTTATTCAGATATTTCGATGt                                                           <  1:205155/141‑1 (MQ=14)

                                                                          cGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCCCTGAGCTTATTCAGATATTTCGATGTa                                                          >  2:353521/1‑141 (MQ=255)

                                                                           ggTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGCCGGTAATCTTGCTAATTGAGGTTAGTAAGTTATTAGGAAGTAc                                                         >  1:89138/1‑141 (MQ=11)

                                                                            gTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACa                                                        >  2:167203/1‑141 (MQ=255)

                                                                             ttCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACaa                                                       >  2:205968/1‑141 (MQ=255)

                                                                                    tatatgTAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATCGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                                <  1:181588/141‑1 (MQ=14)

                                                                                     atataGTGCGTAAATACACTCTATAACTTTTAAAGTATTCTTTGCTGTATTGATACTTTTGTACCTTGTCTCTGTTACTTTATTATGACCGTGATTTTGCTCTATGAGGTTATTCCGTTATTTTGATATACATTggccggc                                               >  2:19215/1‑135 (MQ=2)

                                                                                     atataGAGCGTAAATACACGCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATAACGGTGATCTTGCTCAATGAGGTTATTCCGATATTTCGATGTACAATGGCAGTc                                               >  1:221322/1‑141 (MQ=14)

                                                                                        taGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTGAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAgg                                            <  2:307543/141‑1 (MQ=255)

                                                                                         agagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGCTACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgt                                           <  1:109850/141‑1 (MQ=255)

                                                                                         agagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCGGgt                                           >  2:864/1‑141 (MQ=255)

                                                                                          gagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGTTGTACAATGACAGACAGgtt                                          >  1:297681/1‑141 (MQ=255)

                                                                                           agCGTAAATACACTCAATACCTTTTGGATGATTCGTTGCTGTATTGAGACTTTGATACCTTTTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttt                                         >  1:94201/1‑141 (MQ=14)

                                                                                              gTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttagt                                      >  2:286123/1‑139 (MQ=255)

                                                                                                aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGAAAGTCAGgtttaagtt                                    >  2:153677/1‑141 (MQ=255)

                                                                                                aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                    <  2:61493/141‑1 (MQ=255)

                                                                                                aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                    <  1:17449/141‑1 (MQ=255)

                                                                                                aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                    <  1:145996/141‑1 (MQ=255)

                                                                                                aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGAGATTTCGATGTACAATGGCAGTCAGgtgtaagtt                                    >  1:13957/1‑141 (MQ=255)

                                                                                                  aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                  >  1:35596/1‑141 (MQ=255)

                                                                                                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                 >  1:213938/1‑141 (MQ=255)

                                                                                                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAGACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgattaagtttaa                                 >  2:254354/1‑141 (MQ=255)

                                                                                                    acactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa                                >  2:7515/5‑141 (MQ=255)

                                                                                                    acacTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGCTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa                                >  2:326071/1‑141 (MQ=255)

                                                                                                    aCATTCAATACCTTTTAAAGTATTCGTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaa                                <  1:15675/141‑1 (MQ=255)

                                                                                                         cAATACCTTTTAAAGTATTCTTTGCTGTATTGATTCTTTTATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCtt                           <  1:72559/141‑1 (MQ=255)

                                                                                                          aaTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCttt                          <  1:304245/141‑1 (MQ=255)

                                                                                                           aTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTa                         >  2:101410/1‑141 (MQ=255)

                                                                                                             aCCTTTTAAAGTATACTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATCTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAt                       >  1:160535/1‑141 (MQ=255)

                                                                                                              ccTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                      >  1:77783/1‑141 (MQ=255)

                                                                                                                ttttAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCCGATATTTCGATTTACACTGACAGTCAGGTTTAAGTTTGAAATGTTTAATTat                    >  1:47636/1‑140 (MQ=14)

                                                                                                                 tttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACt                   >  1:52803/1‑141 (MQ=255)

                                                                                                                              ttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTa      >  1:61063/1‑141 (MQ=255)

                                                                                                                              ttGCTGTATTGATACTTTGATACCTTGTCTTTCGTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTAGAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTa      >  2:261346/1‑141 (MQ=255)

                                                                                                                                tcTGTAATGATACTTTGATACCTTGTCTTTTTTACTTTAATATGACGGTGATCTTTCTTAATGAGGTTATTCAGATCTTTCGATGTAAAATGGCAGTCATGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTaac    <  2:109139/140‑3 (MQ=14)

                                                                                                                                gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc    >  2:332586/1‑141 (MQ=255)

                                                                                                                                gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc    >  1:228393/1‑141 (MQ=255)

                                                                                                                                gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc    >  2:248015/1‑141 (MQ=255)

                                                                                                                                 cTGTATTGATACTTTGATACTTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGCAGTTACTCAGATTTTTGGATGTACTATGACAGTCATGTTTAGGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCt   <  1:273493/141‑1 (MQ=14)

                                                                                                                                 cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAACGCCGTCAGCGATAAGTTTAAAAGCTTTAATTACTTTAACAATTGCTACCt   >  2:179589/1‑141 (MQ=14)

                                                                                                                                  tGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCtt  >  2:303786/1‑141 (MQ=255)

CAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTT  >  CP000730/35867‑36137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`