Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,863:1 +T 46.3% intergenic (‑946/‑222) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8621.T46.3% 226.4 / 306.2 136intergenic (‑945/‑223)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (36/37);  new base T (29/34);  total (65/71)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-05

Only 100 of 136 total aligned reads displayed.

GTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCC  >  CP000730/1929728‑1929992
                                                                                                                                       |                                                                                                                                       
gtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑t                                                                                                                                <  1:393039/141‑1 (MQ=255)
  aGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGCGTA‑TAA‑A‑G‑T‑Ga                                                                                                                             >  1:101414/1‑141 (MQ=255)
     cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTAGATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGc                                                                                                                         <  1:275194/141‑1 (MQ=255)
        ttCAATCTATAAGCGAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTa                                                                                                                      >  2:268194/1‑141 (MQ=255)
        ttCAATCTATAAGCAAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTa                                                                                                                      >  2:268215/1‑141 (MQ=255)
            aTCTATCAGCTAGAATCATAGAACTTATCAGAAGCAAGTGACTGGGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAGCGCTCAGGCGTCAATTTAGACTCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTAtttt                                                                                                                  >  2:216176/1‑141 (MQ=255)
                    acTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTcaca                                                                                                          <  1:28266/140‑1 (MQ=255)
                           ggatctcagtccacAGATGCAAGTGACTGTGCTCGTATCTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGc                                                                                                   >  2:86747/14‑141 (MQ=255)
                                       tCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GG‑TGCTTATTTTCGTTCACATCATAGAACCAGCcagcag                                                                                       >  2:174291/1‑141 (MQ=255)
                                          gAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGAACCAGTCATCAGTgg                                                                                    >  1:61762/1‑141 (MQ=255)
                                          atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCTTAGCACCAGTCATCAGTGGc                                                                                   <  1:239216/127‑1 (MQ=255)
                                          atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATCGCCCCAGTCATCAGGGGc                                                                                   >  2:230999/15‑141 (MQ=255)
                                            cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGAtg                                                                                 >  2:293589/13‑141 (MQ=255)
                                             ggaagtcgatatgCTTGTATATTCACAGCTGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTCCT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                <  1:164810/130‑1 (MQ=255)
                                              gaagtagatatgCTTGTATATTCACAGGTGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtc                                                                               >  1:85308/11‑140 (MQ=255)
                                               gagtagatattgttGTACATTCACGGATGGTATAATAATAGAGTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTGTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                              <  2:351849/129‑1 (MQ=255)
                                               aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                              <  1:361961/132‑1 (MQ=255)
                                               aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                              <  1:211653/132‑1 (MQ=255)
                                                agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGACGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                             <  1:268087/133‑2 (MQ=255)
                                                 gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                            >  1:175061/8‑139 (MQ=255)
                                                 gtaaatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                            <  2:153668/134‑3 (MQ=255)
                                                 gTGACTGTGCTTGTATATTTGGCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCCCATCATCGCACTAGTCATCAGTGGCTGTGcc                                                                             >  1:203355/1‑141 (MQ=255)
                                                 gTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGcc                                                                             <  1:204145/141‑1 (MQ=255)
                                                  tGACCGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑G‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGccc                                                                            >  1:269461/1‑140 (MQ=255)
                                                   agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCAACAGTCATCAGTGGCTGTGctgtt                                                                          >  2:18440/6‑137 (MQ=255)
                                                    gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                         <  2:372132/137‑6 (MQ=255)
                                                    gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                         >  1:367696/5‑136 (MQ=255)
                                                    gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                         <  2:175540/137‑6 (MQ=255)
                                                      tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                        >  1:262361/1‑141 (MQ=255)
                                                          cTTGTATAGTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                   <  2:21179/141‑4 (MQ=255)
                                                           ttGTATATTCACAGATGGTTTAATAATAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                                                                  <  2:4917/141‑5 (MQ=255)
                                                            tGTATATTTGCCGATGATATAATAGGAGCGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GC‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                                  <  1:41819/141‑1 (MQ=255)
                                                            tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCTTCAGTGGCTGTGCTGTTGCGtattat                                                                 <  1:375820/141‑6 (MQ=255)
                                                            tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAAGCATATATTT‑‑CTTTAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                                 <  1:11342/141‑6 (MQ=255)
                                                              tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttttt                                                                <  1:396339/141‑1 (MQ=255)
                                                              tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                               <  2:300593/141‑8 (MQ=255)
                                                              tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                               <  2:170422/141‑8 (MQ=255)
                                                              tataTTCACAGATGGTATAATAATAGAGCCGCCTATCTCTCAGGCGTCAATT‑‑GACCAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                               <  1:159110/141‑5 (MQ=255)
                                                                 attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                            >  2:327679/8‑141 (MQ=255)
                                                                 attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑T‑TACTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                            >  1:379275/8‑141 (MQ=255)
                                                                   ttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑CGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAt                                                           <  1:60499/141‑1 (MQ=255)
                                                                    cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGCGCTGTTGCGTATTATACGTATTg                                                         >  1:210517/5‑141 (MQ=255)
                                                                    cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATCCTTATTg                                                         >  1:134399/5‑141 (MQ=255)
                                                                    cacagatggtATAATAATAGAGTCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                         >  1:331669/5‑141 (MQ=255)
                                                                    cacagacggaaaaataatAGATTCGCCTATCATTCAGGCGTAAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                         <  1:138513/129‑1 (MQ=255)
                                                                      cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTGC‑T‑T‑T‑GC‑TAATCATTTTCGTTCAGATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTCTTATACTTATTggt                                                       >  2:223025/3‑140 (MQ=255)
                                                                      cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                       >  1:154708/3‑141 (MQ=255)
                                                                          tggtataATCATAGGGCCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                   <  2:173884/138‑4 (MQ=255)
                                                                          tggtagaataatAGAGTCGCTTATCTCTCAGGCGTCAATT‑‑GACGGAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TACTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                   <  2:69275/135‑4 (MQ=255)
                                                                          tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTTTTGGCTaa                                                    >  2:63992/1‑141 (MQ=255)
                                                                           ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGcctagt                                                  <  2:240761/139‑6 (MQ=255)
                                                                           ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                  <  1:198195/139‑1 (MQ=255)
                                                                           ggtataATAATAGAGTCGCCTATCGCTCAGTCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                  <  2:276286/139‑1 (MQ=255)
                                                                           gatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                   >  2:323567/1‑141 (MQ=255)
                                                                            gtataATAATAGAGTCGCCTATCCCTCAGGCGCCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                 <  1:755/140‑1 (MQ=255)
                                                                            atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACAGCGTAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt                                                  >  2:217750/1‑141 (MQ=255)
                                                                             tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCCCATCATAGCACCAGTCATCAGTAGCTGTGCTGTTGCGTATGATACTTATTGGCTGAGTaa                                                >  2:236414/1‑140 (MQ=255)
                                                                             tataATAATAGAGTCGCCTATCTCTCAGGCGTAAATT‑‑GACGAAGAGCGGAGGTGCA‑TTA‑G‑T‑TAGT‑TACTCATTTTGGCTTATATCATAGAACCAGTTCTCAGTGGCTGTGTGTTTGCTGTTTATACTTATTGGCTTAGTaa                                                >  2:329658/1‑140 (MQ=255)
                                                                              ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACTAAGAGAGGAGGTGCA‑TTTCT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                               <  1:178075/141‑3 (MQ=255)
                                                                              ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGCAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACGAGTCATCAGTGGCTGTGCTGTTGCGGATTATACTTATTGGCTTAGTaaa                                               >  2:86108/1‑139 (MQ=255)
                                                                              ataataataGAGTCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGGGGTGCA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                               <  1:99730/141‑3 (MQ=255)
                                                                                 ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAA‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                            >  1:32389/5‑141 (MQ=255)
                                                                                 ataataGCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                            <  1:107586/141‑1 (MQ=255)
                                                                                 ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                            <  1:120251/141‑1 (MQ=255)
                                                                                 ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAATCACTAAGTTGAcc                                             >  1:148590/1‑140 (MQ=255)
                                                                                 agaagagcggcGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCAGCAGTGGCTTTGCCGTTGCTTATTATACTTATTGGCTTAGTAAACGc                                            <  2:143877/131‑1 (MQ=255)
                                                                                  atgtagAGTCGTCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGc                                            <  1:267220/139‑1 (MQ=255)
                                                                                  aaatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                           <  2:203161/138‑1 (MQ=255)
                                                                                   aatcgagTCGCCTCTCTCTCAGGCGTCAATT‑‑GACGAAGCGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                          <  1:99205/137‑1 (MQ=255)
                                                                                   aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                          >  1:69935/3‑141 (MQ=255)
                                                                                   aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCAGAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                          <  1:59507/139‑1 (MQ=255)
                                                                                   aatagAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCCCCCATTAGTAGTGGCTGGTTTCTTTCGTATTATACTTATTGGCTTAGTAAACGCaa                                          <  1:92973/139‑1 (MQ=255)
                                                                                      agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                       >  1:231743/1‑138 (MQ=255)
                                                                                      agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                       <  2:360076/141‑4 (MQ=255)
                                                                                      agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGAGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACAGCATAGCACCAGTCATCAGTGGCTGTGCTGCTGCGTAGTATACTTCTTGGATTAGTAAACGCaacaa                                       >  2:169649/1‑138 (MQ=255)
                                                                                      agagTCCCCTATTTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                       <  2:10224/141‑4 (MQ=255)
                                                                                           cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GT‑TAATCATTTTCGTTCACATCATAGCTCCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTGAGTCAACGCaacaaataat                                  <  1:294894/141‑9 (MQ=255)
                                                                                            gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAGGCaacaaataatt                                 >  2:223362/1‑132 (MQ=255)
                                                                                            gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGccacaaataatt                                 >  2:128686/1‑130 (MQ=255)
                                                                                             ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGGAGCAATACCAAATa                                 >  2:350998/1‑141 (MQ=255)
                                                                                             ccTATCTCTCAGGCATCAAATTAGAAGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                                 >  1:216671/1‑141 (MQ=255)
                                                                                              cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                               <  2:367696/141‑12 (MQ=255)
                                                                                               tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                              <  2:315908/141‑13 (MQ=255)
                                                                                               tATCTCTCAGGCGTCAACT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                              <  1:359212/141‑13 (MQ=255)
                                                                                                aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGt                              <  1:326067/141‑1 (MQ=255)
                                                                                                aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATCCCTATTAGCTTAGTAAACGaaacaaataattaata                             >  1:304464/1‑128 (MQ=255)
                                                                                                aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                             >  1:149819/1‑128 (MQ=255)
                                                                                                aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                             >  2:134852/1‑128 (MQ=255)
                                                                                                aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                             >  2:349620/1‑128 (MQ=255)
                                                                                                aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaaaa                             >  1:385276/1‑128 (MQ=255)
                                                                                                aTCTCTCAGGCGTCAATT‑‑GAAGAAGAGAGGAGGTGCA‑TTA‑C‑T‑T‑TACTAATCATTTTCGCTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGTaacaaataattaata                             >  1:240248/1‑128 (MQ=255)
                                                                                                 tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                            <  1:323452/141‑15 (MQ=255)
                                                                                                      cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                        <  1:273782/141‑1 (MQ=255)
                                                                                                       aGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACatat                       <  2:262361/141‑1 (MQ=255)
                                                                                                        ggCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACAtata                      >  1:159331/1‑141 (MQ=255)
                                                                                                         gCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAg                     <  1:335541/141‑1 (MQ=255)
                                                                                                               aTTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTTAGTAGACGCAATATAAAATAGGTGACATATAGCCGCAc               <  1:254576/141‑1 (MQ=255)
                                                                                                               aTTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCAc               >  1:307362/1‑141 (MQ=255)
                                                                                                                     aCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaa         <  2:287627/141‑1 (MQ=255)
                                                                                                                            gaggagGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATccc  <  1:86747/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCC  >  CP000730/1929728‑1929992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑