BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,913	C→G	15.6%	S213S (TCG→TCC)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,913	0	C	G	15.6%	359.6 / 79.5	217	S213S (TCG→TCC)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (72/109); new base G (19/15); total (92/125)
Fisher's exact test for biased strand distribution p-value = 9.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

Only 100 of 225 total aligned reads displayed.

TTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCT  >  CP000730/35774‑36052

     cAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaaaa                                                                                                                                        <  2:51706/140‑1 (MQ=255)

                acaaaagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGa                                                                                                                           >  1:20869/15‑140 (MQ=11)

                  aaaagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCTGTGTTTGTTTATCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                                                         <  1:194225/128‑1 (MQ=11)

                   aaagtgtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCTCTAATATATCTTGTCAGTGTTCGTTTATCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                                                                                        <  1:74933/129‑1 (MQ=11)

                   aaagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                                                                                        >  2:159932/12‑140 (MQ=11)

                    aagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                                                                                                       <  1:534692/130‑1 (MQ=11)

                     atTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                                                                                        >  2:584782/1‑140 (MQ=255)

                       agtattttaataaAAAT‑GATGGCTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                                                    <  2:294229/133‑1 (MQ=11)

                         tgactatataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATACCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                                                    <  2:307935/133‑1 (MQ=255)

                         tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAGCCGTAGATCTGAAGAGACCTg                                                                                                                  <  1:34158/135‑1 (MQ=11)

                         tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGATAGCATGCTGCTCAGTTCGTGGCATGGCGATAATCCGTAAATCTGCAGAGAcacg                                                                                                                  >  2:329856/6‑137 (MQ=11)

                          attttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGGATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                 <  1:158799/136‑1 (MQ=11)

                          attttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGACAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAGCTGTAGATCTGAAGAGAACTGc                                                                                                                 >  1:118936/5‑140 (MQ=11)

                           ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTACTATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                >  1:46612/4‑140 (MQ=11)

                           ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                <  1:665108/137‑1 (MQ=11)

                           ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                <  2:14986/137‑1 (MQ=11)

                             cTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                <  1:635511/140‑1 (MQ=255)

                              taataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                             <  2:45730/140‑1 (MQ=11)

                               aataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAATATTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                                                            >  1:228945/1‑140 (MQ=11)

                                      atggctgggtCTGTGGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTta                                                                                                      <  2:33711/131‑1 (MQ=1)

                                        tgctttttatGTTGCAAAGTAAAAAAATATAGCGTATCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGTCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat                                                                                                     <  2:454308/131‑1 (MQ=1)

                                        tgAAGGTTCTGTTGAAAAGCAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttttcgt                                                                                                     <  2:504480/139‑4 (MQ=9)

                                                               aacatagAGGTAACTATTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTTTTATGGCGAAAATTCTTATATTTGAAGTGACCTGCAGTTTTTTTTATATAGAGCGTAAATACATTCAATa                                                                              <  1:508092/137‑1 (MQ=1)

                                                                               ctaatacaccATGTTAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTATATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCt                                                              <  1:360623/131‑1 (MQ=1)

                                                                                  atatattatGTCAGTGTTCGTTTACCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGATAGTTGCGGTTCTTGTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTg                                                           <  1:385591/137‑1 (MQ=1)

                                                                                  atatATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCAGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTGAATACGTTTTAAAGTATTCTTTg                                                           >  2:531082/4‑140 (MQ=1)

                                                                                  atatATCAGGTCATTGTTCTTTTAACTTGCAAGCATGATGCTAATTTCCTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATCGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTg                                                           <  1:320431/137‑1 (MQ=1)

                                                                                              aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc                                               <  1:571427/140‑1 (MQ=11)

                                                                                               gttttcgtctAACTTGCTAGCAAGATGCTAATTTCGTGGCATGGCTAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                              <  1:10599/131‑1 (MQ=11)

                                                                                               gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGAGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                              >  1:284000/1‑140 (MQ=11)

                                                                                                tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                             >  2:478886/1‑140 (MQ=255)

                                                                                                tgtTCGCTTAACTTGCTAGCCTTTGGCTTATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                             >  2:642331/1‑140 (MQ=11)

                                                                                                tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                             >  1:306929/1‑140 (MQ=17)

                                                                                                tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                             >  1:494598/1‑140 (MQ=17)

                                                                                                 gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                            <  2:548954/140‑1 (MQ=16)

                                                                                                 gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAACCGTAGATCTGAAAAGACCTGCGGTACTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                            >  1:358220/1‑140 (MQ=11)

                                                                                                 gtTCGCTTAACTTGCTTGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                            <  1:80714/140‑1 (MQ=16)

                                                                                                  ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                           <  2:349688/140‑1 (MQ=16)

                                                                                                  ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAAAGACCTGCGGTTCTTTTTATATAGAGCCTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACATTg                                           >  1:311666/1‑140 (MQ=11)

                                                                                                  ttCGCTTAACTTTCTAGCCTGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATATAGCATAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                           <  2:57776/140‑1 (MQ=11)

                                                                                                   tcgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAAACCTACGGTTCTTTTTATATAGTGTGTAAATACATTCAATACCTTTTAAAGTATTCTTTGGCGTATTGATACTTTGa                                          <  1:330195/136‑1 (MQ=11)

                                                                                                   tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                          <  1:270266/140‑1 (MQ=18)

                                                                                                    cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATGGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGAt                                         >  1:27794/4‑140 (MQ=11)

                                                                                                      tttAACTTGCTAGCATGATGCTAATTTCGTGGAATGGCGAAAATCCGTAGATCTGAAGACCCCTGCGGTTCTTTTTATATAGAGGGTAAATACACTCAATGCCTTTTAAAGTATTCTTTGCTGTATTAATCCTTTGATAc                                       >  2:118076/2‑140 (MQ=11)

                                                                                                      tttAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAGCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTGTTTGCTGTATTGATACTTTGATAc                                       <  2:229148/139‑1 (MQ=255)

                                                                                                      ctcgtattGCGAGCATGATGCGCATTTCGTGGCAGGGCGAAGATTCGTAGATCTGATGAGTCCTGCGGTTTTTGTTATATAGAGCGTAAATATGCTCAATAACTTTTAGGATGTGCTTCGCTTTATTGTTGCTTTGATAc                                       <  2:276762/134‑1 (MQ=2)

                                                                                                       ttAACTTGCTAGCATGATGCTAATTTCTTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                      <  1:214402/140‑1 (MQ=18)

                                                                                                       ttAACTTGCTAGCATGATGCTAATTTCTCGGTATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                      <  1:547860/140‑1 (MQ=255)

                                                                                                         aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                    <  2:545751/140‑1 (MQ=33)

                                                                                                         aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                    <  1:545264/140‑1 (MQ=18)

                                                                                                         aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                    <  1:53145/140‑1 (MQ=18)

                                                                                                          aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAAATCTGAAAAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                   >  1:14828/1‑140 (MQ=12)

                                                                                                           cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGGTACTTTGATACCTTGt                                  >  2:174310/1‑140 (MQ=17)

                                                                                                           cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                  <  1:652014/140‑1 (MQ=18)

                                                                                                           cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                  <  2:598111/140‑1 (MQ=18)

                                                                                                           cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAACttct                                  >  2:635444/1‑138 (MQ=12)

                                                                                                           cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTACTCACCCGAGTGATACTTTGATACCTTGt                                  <  1:159932/140‑1 (MQ=11)

                                                                                                           cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTACATCTGAAGAGAGCTGCGGTTCTTTTTATATAGAGCGTAAACACACTCAATACCTTTTAAAGTTTTCTTTGCTGTATTGATACTTTGCTACCTTGt                                  >  2:365785/1‑140 (MQ=11)

                                                                                                           cTTGCTAGCATGATGCTAATATGGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGGTCGTGTTATATAGAGCGTAAATACACTCAGTACCTTTTGAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                  >  2:194388/1‑140 (MQ=11)

                                                                                                            ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGCCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                 <  2:614156/140‑1 (MQ=18)

                                                                                                              gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                               >  1:247430/1‑140 (MQ=17)

                                                                                                              gCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                               >  1:420186/1‑140 (MQ=18)

                                                                                                               cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATGTTGtcttt                              >  2:259007/1‑140 (MQ=11)

                                                                                                                tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttc                             >  1:590772/1‑140 (MQ=11)

                                                                                                                tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATAACTTTTAAAGAATGGTTTGCCGTATTGATACTTTGATATCTTGtctttc                             >  1:451232/1‑140 (MQ=11)

                                                                                                                tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                             >  2:88740/1‑140 (MQ=17)

                                                                                                                tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                             <  1:446172/140‑1 (MQ=18)

                                                                                                                tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                             <  1:189461/140‑1 (MQ=18)

                                                                                                                tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGAAACTTTGATACCTTGtctttc                             >  2:268720/1‑140 (MQ=17)

                                                                                                                 aGCCTGATCGTATTTCCGTGGCATGGCTAAAATCCGTAGATCTGAAGAGACTTGCGGTTCTTTTTATATAGAGCGTCAATACCCTCAATACCTTTTAAAGTATTCTTTGCTGTATTTATACTTTGATACCTTGtctttct                            <  2:388916/140‑1 (MQ=11)

                                                                                                                  gCATGATGCTAATTTCGTGGCATGGCGACAATCAGGCGCTCTGAAGAGACCTGCTGTGCTTTTGATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGTTACTTTGATACCTTGtctttctt                           >  2:275006/1‑140 (MQ=11)

                                                                                                                  gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                           >  1:29451/1‑140 (MQ=18)

                                                                                                                   cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                          >  1:503188/1‑140 (MQ=18)

                                                                                                                   cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                          >  2:418175/1‑140 (MQ=18)

                                                                                                                     tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCGGTATTGATACTTTGATAGCtttcataagtgacg                        >  2:136003/1‑128 (MQ=11)

                                                                                                                       attgtAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAAAGACCTTCGTTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                      <  2:467755/136‑1 (MQ=11)

                                                                                                                       atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                      >  1:379333/1‑140 (MQ=34)

                                                                                                                       atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                      >  1:558252/1‑140 (MQ=35)

                                                                                                                          cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                   >  2:6277/1‑140 (MQ=25)

                                                                                                                           tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAATTTAATATCTTGTCTTTCTTACTTTAatt                  >  1:600465/1‑139 (MQ=11)

                                                                                                                           tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                  >  1:607009/1‑140 (MQ=25)

                                                                                                                           tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                  >  2:2020/1‑140 (MQ=25)

                                                                                                                            aaTTTGGTGGAATGGCGAAAAGCCGTAGATCTGAAGAGACCTGCGGTTTTCTTTCTAGATAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTCatat                 <  1:454309/140‑1 (MQ=11)

                                                                                                                            aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGCGACGTTCGTTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                 >  1:627661/1‑140 (MQ=11)

                                                                                                                             ttgtCGGGGCATGGCGAAAATCCGTAGATCTGAAGTGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATGTCTTGTCTTTCTTACTTTAATATg                <  2:665054/137‑1 (MQ=11)

                                                                                                                             aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                >  1:141332/1‑140 (MQ=21)

                                                                                                                               ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAc              <  2:43838/140‑1 (MQ=37)

                                                                                                                                tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg             <  1:251520/140‑1 (MQ=37)

                                                                                                                                  gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt           >  1:26738/1‑140 (MQ=38)

                                                                                                                                  gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCCGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAATATTCTTTGCTGTATTGATACTTTGATAACTTGTCTTTCTTACTGTAATATGACGGt           >  1:114632/1‑140 (MQ=255)

                                                                                                                                   tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg          >  1:662628/1‑140 (MQ=38)

                                                                                                                                   tGGCAGGGCGAAAATCCTTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg          <  2:247857/140‑1 (MQ=255)

                                                                                                                                    ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACGCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCCTTCTTACTTTAATCTAACGGTGa         >  2:109051/1‑140 (MQ=11)

                                                                                                                                    ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa         <  2:563362/140‑1 (MQ=255)

                                                                                                                                        tGGCGAAAATCCGTCGATCTGAAGATACCTGCGCTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAGTTTAATATGACGGTGATCtt     <  1:404909/140‑1 (MQ=11)

                                                                                                                                         ggCGAAAATCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTTAATACCTTTTAAAGTATTCTTTGCTGTATTGAGACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg    <  1:102097/140‑1 (MQ=255)

                                                                                                                                         ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGCTACCTTGTCTTTCTTACTTTAATATGACGGTCATCTTg    >  2:609582/1‑140 (MQ=255)

                                                                                                                                          gCGAAAATCCGTAGATCTGAAGAGACCTGTGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc   <  1:392957/140‑1 (MQ=255)

                                                                                                                                           cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTCATTTAATATGACGGTGATCTTGCt  >  1:236839/1‑140 (MQ=255)

                                                                                                                                           cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt  >  2:24202/1‑140 (MQ=255)

TTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCT  >  CP000730/35774‑36052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`