Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,799 C→A 35.8% intergenic (‑882/‑286) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,7990CA35.8% 84.0 / 70.1 97intergenic (‑882/‑286)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (34/28);  new base A (13/22);  total (47/50)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

GTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTG  >  CP000730/1929660‑1929937
                                                                                                                                           |                                                                                                                                              
gTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGAGTGTATATTTGcc                                                                                                                                                >  1:117695/1‑140 (MQ=255)
     aataaAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCgatga                                                                                                                                           <  1:649753/140‑1 (MQ=255)
     aataaAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCgatga                                                                                                                                           <  1:79461/140‑1 (MQ=255)
      ataaAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCgatgat                                                                                                                                          <  2:259406/140‑1 (MQ=255)
          aaTCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCgatgattaac                                                                                                                                      >  2:327669/1‑136 (MQ=255)
          aaTCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATataa                                                                                                                                      >  2:398272/1‑140 (MQ=255)
          aaTCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTCTGCTTCTATATTTGCCgcgtgaataa                                                                                                                                      >  2:290266/1‑131 (MQ=25)
          aaTCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAAATTATCAGAAGCAAGTGACTGTGCTTGTATATTTTCCGATGATataa                                                                                                                                      >  2:327701/1‑140 (MQ=255)
              gCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagt                                                                                                                                  >  1:658037/1‑140 (MQ=255)
                  aCTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTagag                                                                                                                              >  1:375683/1‑140 (MQ=255)
                     ttCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATATTATAGTCg                                                                                                                           >  2:359776/1‑140 (MQ=255)
                          aaaTTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAt                                                                                                                      >  2:546191/1‑140 (MQ=255)
                           aaTTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAtc                                                                                                                     >  2:523940/1‑140 (MQ=255)
                             ttAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTCTCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAtctc                                                                                                                   <  2:597648/140‑1 (MQ=255)
                             ttAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAAAAACAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCATAtctc                                                                                                                   >  1:43113/1‑140 (MQ=255)
                                aCAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCa                                                                                                                >  2:195487/1‑140 (MQ=255)
                                 cAATATCTATTCTCATAGAATTTGTCCAATTAAGTATAGGCAAGTATATCTATCAACTAGAATCATATAACTTATAAAAAGCAAGTGACTGGGCTTGTATATTTGTCGATGATATAAAAGGAGAGTCACCTaattctacg                                                                                                               >  2:437332/1‑132 (MQ=25)
                                     atCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCAAGTATATTTGCCGATGATATAATAGTAAAGTCGCCTCACTCTCAGGCGt                                                                                                           >  1:58478/1‑140 (MQ=38)
                                     atCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAACTAGAATCATATAACTTATCAGAAGCAAGTGACTCTGCTTGTATATTTGCCGATGATAGAATAGTAGAGTCGCCTATCTCTCAGGCGt                                                                                                           >  1:355065/1‑140 (MQ=255)
                                      tCTATTCTCATAGAATTTGCCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTc                                                                                                          <  2:343822/140‑1 (MQ=255)
                                        tATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCaa                                                                                                        >  1:607591/1‑140 (MQ=255)
                                           tctcAGAGAATTTGCCCAATTAAGTGTAGACGATTCAATCTAGCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAttt                                                                                                     <  2:176771/140‑1 (MQ=255)
                                               aTAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTGGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGAc                                                                                                 >  2:637807/1‑140 (MQ=255)
                                                tAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTAACAAAAGAAAGTGACTGAGCTTGTAACTTTGCCGATGATATAATAATAGAGTCGCCTATATCCCAGGCGACAATTTAGaag                                                                                                >  2:72305/1‑138 (MQ=255)
                                                   aaTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGccg                                                                                             >  1:663178/1‑138 (MQ=255)
                                                       tGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCTTATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                         <  1:87393/140‑1 (MQ=255)
                                                          ctaagaaaGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAACAGCAAGTGACTGTGCTTGTATAGTTGCCGATGATATAATAGTAGAGTCACCTATCTCTCAGGCGTCAATTTAGATGCAGAgagggg                                                                                      >  2:173036/7‑138 (MQ=255)
                                                               tAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGta                                                                                 >  2:208517/1‑140 (MQ=255)
                                                                  gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAATAGAATCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGAGTATaa                                                                             >  1:324369/1‑140 (MQ=255)
                                                                   tgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAg                                                                           >  1:177297/1‑140 (MQ=255)
                                                                         cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATg                                                                   <  1:107495/140‑1 (MQ=255)
                                                                            ttCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCtt                                                                >  2:116665/1‑140 (MQ=255)
                                                                            ttCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCtt                                                                <  1:455596/140‑1 (MQ=255)
                                                                            ttCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCACCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCtt                                                                >  2:180334/1‑140 (MQ=255)
                                                                              cAATCTATCAGCTAGCATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt                                                              >  1:495638/1‑140 (MQ=255)
                                                                              cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt                                                              <  1:416060/140‑1 (MQ=255)
                                                                               aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCTTATCTCTCCGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAtt                                                             <  1:28187/140‑1 (MQ=255)
                                                                               aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGCGTATACGGTGATGCTTAtt                                                             >  2:420496/1‑140 (MQ=255)
                                                                                aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAttt                                                            >  2:127954/1‑140 (MQ=255)
                                                                                 tCTATCAGCTTGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAtttt                                                           <  1:239395/140‑1 (MQ=255)
                                                                                 tCTATCAGCTAGAATTATATAACTTATCAGAAGCAATTTACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAtttt                                                           <  1:285190/140‑1 (MQ=255)
                                                                                   tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCg                                                         <  1:327701/140‑1 (MQ=255)
                                                                                   tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCg                                                         <  1:398272/140‑1 (MQ=255)
                                                                                   tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCg                                                         <  1:327669/140‑1 (MQ=255)
                                                                                     tCATCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGtt                                                       <  1:213811/140‑1 (MQ=255)
                                                                                         cTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTcaca                                                   <  1:195487/140‑1 (MQ=255)
                                                                                             aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCAcatcat                                               <  1:523940/140‑1 (MQ=255)
                                                                                                 atatAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCa                                           <  1:599815/140‑1 (MQ=255)
                                                                                                 atatAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCATATTTTCGTTCACATCATAGcc                                           >  2:601680/1‑139 (MQ=255)
                                                                                                     aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAg                                       <  2:232125/140‑1 (MQ=255)
                                                                                                            cAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAg                                >  1:642826/1‑140 (MQ=255)
                                                                                                             aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTCGTTCACATCATAGCACCAGTCATCAGTg                              <  2:561362/140‑1 (MQ=255)
                                                                                                              atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAATCAACAGTgg                             >  2:194350/15‑140 (MQ=255)
                                                                                                              atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCAACAGTgg                             <  2:592135/126‑1 (MQ=255)
                                                                                                              atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTgg                             >  2:553749/15‑140 (MQ=255)
                                                                                                              agcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTgg                             <  2:186851/126‑1 (MQ=255)
                                                                                                               tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATAATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                            >  1:80227/14‑140 (MQ=255)
                                                                                                                 ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                          >  1:609342/12‑140 (MQ=255)
                                                                                                                 ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                          <  2:564867/129‑1 (MQ=255)
                                                                                                                  gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGTGTTGACTAACTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                         <  1:314332/130‑1 (MQ=255)
                                                                                                                   aagtagatatggttGTATATTCACAGATGGTATAATAACAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                        <  2:392132/128‑1 (MQ=255)
                                                                                                                   aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                        <  2:125944/131‑1 (MQ=255)
                                                                                                                   aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                        <  1:506592/131‑1 (MQ=255)
                                                                                                                    agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                       <  1:512835/132‑1 (MQ=255)
                                                                                                                    agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATTAACGAAGAGAGAAGGAGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                       >  1:67708/9‑140 (MQ=255)
                                                                                                                    agtacatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                       <  2:484059/132‑1 (MQ=255)
                                                                                                                    aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                        >  2:506101/1‑140 (MQ=255)
                                                                                                                    aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                        <  1:519469/140‑1 (MQ=255)
                                                                                                                    aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCggtg                        >  2:343325/1‑140 (MQ=255)
                                                                                                                    aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGAGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGATTGTTTTCGTTCACATCATAGCGCCAGACATCAGTGGCtgtg                        >  1:179563/1‑140 (MQ=255)
                                                                                                                    aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGACGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                        >  2:240119/1‑140 (MQ=255)
                                                                                                                     gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCCCCCCAAACCACCAGTCATCAGTGGCTGTGct                      <  1:490424/133‑2 (MQ=255)
                                                                                                                     gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                      <  2:654528/133‑2 (MQ=255)
                                                                                                                     gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                      >  1:97691/8‑139 (MQ=255)
                                                                                                                     gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                      <  1:312212/133‑2 (MQ=255)
                                                                                                                     gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACATTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                      >  1:478801/8‑139 (MQ=255)
                                                                                                                      tagatatgCTTGTATATTTACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                     <  1:169529/134‑3 (MQ=255)
                                                                                                                      tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGcc                      <  1:249326/140‑1 (MQ=255)
                                                                                                                       agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GTCGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                    <  1:230962/135‑4 (MQ=255)
                                                                                                                       agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                    <  1:118944/135‑4 (MQ=255)
                                                                                                                       agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                    <  2:25474/135‑4 (MQ=255)
                                                                                                                        gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtgatgtt                   >  2:484475/5‑135 (MQ=255)
                                                                                                                        gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                   >  2:103259/5‑136 (MQ=255)
                                                                                                                        gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                   <  1:246185/136‑5 (MQ=255)
                                                                                                                        aCTGCGCTTCTATATTTGCCGATGATATAATAGTAGAGTCGCCTATTTCTCAGGCGTTAATTTAGACGTAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                    <  1:315532/140‑1 (MQ=255)
                                                                                                                         cTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                   >  1:407211/1‑140 (MQ=255)
                                                                                                                         atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                  >  1:602345/4‑135 (MQ=255)
                                                                                                                         atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                  >  1:236127/4‑135 (MQ=255)
                                                                                                                          tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATTAACGAAGAAAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtttc                 >  1:554713/3‑134 (MQ=255)
                                                                                                                          tatgCTTGTATATTCACAGAGGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                 <  2:267487/138‑7 (MQ=255)
                                                                                                                           gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTTTCGCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                 <  1:123323/140‑1 (MQ=255)
                                                                                                                           atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGAACCAGTCATCAGTGGCTGTGCTGTTGCg                >  1:336112/2‑140 (MQ=255)
                                                                                                                            tgCTTGTATATTTGCCGATGACAGAATAGTAGAGTCGTCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTTTATAAGGTGATGCTTTTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                <  2:86822/140‑1 (MQ=255)
                                                                                                                               ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt            <  1:500520/140‑4 (MQ=255)
                                                                                                                               ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt            <  2:340199/140‑4 (MQ=255)
                                                                                                                                tGTATATTCACAAATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta           <  1:59706/140‑5 (MQ=255)
                                                                                                                                 gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat          <  1:652921/140‑3 (MQ=255)
                                                                                                                                  tataTTTGCCGATGAAATAATAGGAGAGTTGCCTATCTCTCAGGCGTCAATTTAGTCGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCCATTGCGtttttt          <  1:261145/140‑1 (MQ=255)
                                                                                                                                          ccGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTg  >  2:156940/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                              
GTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTG  >  CP000730/1929660‑1929937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: