Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,796 T→C 34.4% intergenic (‑879/‑289) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,7960TC34.4% 80.5 / 88.1 99intergenic (‑879/‑289)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (36/29);  new base C (13/21);  total (49/50)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

CTTGTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTT  >  CP000730/1929657‑1929929
                                                                                                                                           |                                                                                                                                         
ctTGTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATAttt                                                                                                                                           >  1:36972/1‑140 (MQ=255)
 ttGTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGAGACTGTGCTTGTATATTTg                                                                                                                                          >  1:86822/1‑140 (MQ=255)
   gTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGAGTGTATATTTGcc                                                                                                                                        >  1:117695/1‑140 (MQ=255)
        aataaAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCgatga                                                                                                                                   <  1:79461/140‑1 (MQ=255)
        aataaAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCgatga                                                                                                                                   <  1:649753/140‑1 (MQ=255)
         ataaAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCgatgat                                                                                                                                  <  2:259406/140‑1 (MQ=255)
             aaTCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCgatgattaac                                                                                                                              >  2:327669/1‑136 (MQ=255)
             aaTCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATataa                                                                                                                              >  2:398272/1‑140 (MQ=255)
             aaTCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTCTGCTTCTATATTTGCCgcgtgaataa                                                                                                                              >  2:290266/1‑131 (MQ=25)
             aaTCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAAATTATCAGAAGCAAGTGACTGTGCTTGTATATTTTCCGATGATataa                                                                                                                              >  2:327701/1‑140 (MQ=255)
                 gCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagt                                                                                                                          >  1:658037/1‑140 (MQ=255)
                     aCTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTagag                                                                                                                      >  1:375683/1‑140 (MQ=255)
                        ttCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATATTATAGTCg                                                                                                                   >  2:359776/1‑140 (MQ=255)
                             aaaTTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAt                                                                                                              >  2:546191/1‑140 (MQ=255)
                              aaTTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAtc                                                                                                             >  2:523940/1‑140 (MQ=255)
                                ttAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTCTCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAtctc                                                                                                           <  2:597648/140‑1 (MQ=255)
                                ttAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAAAAACAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCATAtctc                                                                                                           >  1:43113/1‑140 (MQ=255)
                                   aCAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCa                                                                                                        >  2:195487/1‑140 (MQ=255)
                                    cAATATCTATTCTCATAGAATTTGTCCAATTAAGTATAGGCAAGTATATCTATCAACTAGAATCATATAACTTATAAAAAGCAAGTGACTGGGCTTGTATATTTGTCGATGATATAAAAGGAGAGTCACCTaattctacg                                                                                                       >  2:437332/1‑132 (MQ=25)
                                        atCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCAAGTATATTTGCCGATGATATAATAGTAAAGTCGCCTCACTCTCAGGCGt                                                                                                   >  1:58478/1‑140 (MQ=38)
                                        atCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAACTAGAATCATATAACTTATCAGAAGCAAGTGACTCTGCTTGTATATTTGCCGATGATAGAATAGTAGAGTCGCCTATCTCTCAGGCGt                                                                                                   >  1:355065/1‑140 (MQ=255)
                                         tCTATTCTCATAGAATTTGCCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTc                                                                                                  <  2:343822/140‑1 (MQ=255)
                                           tATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCaa                                                                                                >  1:607591/1‑140 (MQ=255)
                                              tctcAGAGAATTTGCCCAATTAAGTGTAGACGATTCAATCTAGCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAttt                                                                                             <  2:176771/140‑1 (MQ=255)
                                                  aTAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTGGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGAc                                                                                         >  2:637807/1‑140 (MQ=255)
                                                   tAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTAACAAAAGAAAGTGACTGAGCTTGTAACTTTGCCGATGATATAATAATAGAGTCGCCTATATCCCAGGCGACAATTTAGaag                                                                                        >  2:72305/1‑138 (MQ=255)
                                                      aaTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGccg                                                                                     >  1:663178/1‑138 (MQ=255)
                                                          tGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCTTATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                 <  1:87393/140‑1 (MQ=255)
                                                             ctaagaaaGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAACAGCAAGTGACTGTGCTTGTATAGTTGCCGATGATATAATAGTAGAGTCACCTATCTCTCAGGCGTCAATTTAGATGCAGAgagggg                                                                              >  2:173036/7‑138 (MQ=255)
                                                                  tAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGta                                                                         >  2:208517/1‑140 (MQ=255)
                                                                     gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAATAGAATCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGAGTATaa                                                                     >  1:324369/1‑140 (MQ=255)
                                                                      tgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAg                                                                   >  1:177297/1‑140 (MQ=255)
                                                                            cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATg                                                           <  1:107495/140‑1 (MQ=255)
                                                                               ttCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCtt                                                        >  2:116665/1‑140 (MQ=255)
                                                                               ttCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCtt                                                        <  1:455596/140‑1 (MQ=255)
                                                                               ttCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCACCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCtt                                                        >  2:180334/1‑140 (MQ=255)
                                                                                 cAATCTATCAGCTAGCATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt                                                      >  1:495638/1‑140 (MQ=255)
                                                                                 cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt                                                      <  1:416060/140‑1 (MQ=255)
                                                                                  aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCTTATCTCTCCGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAtt                                                     <  1:28187/140‑1 (MQ=255)
                                                                                  aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGCGTATACGGTGATGCTTAtt                                                     >  2:420496/1‑140 (MQ=255)
                                                                                   aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAttt                                                    >  2:127954/1‑140 (MQ=255)
                                                                                    tCTATCAGCTTGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAtttt                                                   <  1:239395/140‑1 (MQ=255)
                                                                                    tCTATCAGCTAGAATTATATAACTTATCAGAAGCAATTTACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAtttt                                                   <  1:285190/140‑1 (MQ=255)
                                                                                      tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCg                                                 <  1:327701/140‑1 (MQ=255)
                                                                                      tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCg                                                 <  1:327669/140‑1 (MQ=255)
                                                                                      tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCg                                                 <  1:398272/140‑1 (MQ=255)
                                                                                        tCATCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGtt                                               <  1:213811/140‑1 (MQ=255)
                                                                                            cTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTcaca                                           <  1:195487/140‑1 (MQ=255)
                                                                                                aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCAcatcat                                       <  1:523940/140‑1 (MQ=255)
                                                                                                    atatAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCa                                   <  1:599815/140‑1 (MQ=255)
                                                                                                    atatAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCATATTTTCGTTCACATCATAGcc                                   >  2:601680/1‑139 (MQ=255)
                                                                                                        aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAg                               <  2:232125/140‑1 (MQ=255)
                                                                                                               cAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAg                        >  1:642826/1‑140 (MQ=255)
                                                                                                                aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTCGTTCACATCATAGCACCAGTCATCAGTg                      <  2:561362/140‑1 (MQ=255)
                                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAATCAACAGTgg                     >  2:194350/15‑140 (MQ=255)
                                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCAACAGTgg                     <  2:592135/126‑1 (MQ=255)
                                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTgg                     >  2:553749/15‑140 (MQ=255)
                                                                                                                 agcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTgg                     <  2:186851/126‑1 (MQ=255)
                                                                                                                  tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATAATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                    >  1:80227/14‑140 (MQ=255)
                                                                                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                  <  2:564867/129‑1 (MQ=255)
                                                                                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                  >  1:609342/12‑140 (MQ=255)
                                                                                                                     gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGTGTTGACTAACTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                 <  1:314332/130‑1 (MQ=255)
                                                                                                                      aagtagatatggttGTATATTCACAGATGGTATAATAACAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                <  2:392132/128‑1 (MQ=255)
                                                                                                                      aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                <  2:125944/131‑1 (MQ=255)
                                                                                                                      aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                <  1:506592/131‑1 (MQ=255)
                                                                                                                       agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc               <  1:512835/132‑1 (MQ=255)
                                                                                                                       agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATTAACGAAGAGAGAAGGAGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc               >  1:67708/9‑140 (MQ=255)
                                                                                                                       agtacatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc               <  2:484059/132‑1 (MQ=255)
                                                                                                                       aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                <  1:519469/140‑1 (MQ=255)
                                                                                                                       aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                >  2:506101/1‑140 (MQ=255)
                                                                                                                       aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCggtg                >  2:343325/1‑140 (MQ=255)
                                                                                                                       aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGAGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGATTGTTTTCGTTCACATCATAGCGCCAGACATCAGTGGCtgtg                >  1:179563/1‑140 (MQ=255)
                                                                                                                       aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGACGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                >  2:240119/1‑140 (MQ=255)
                                                                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCCCCCCAAACCACCAGTCATCAGTGGCTGTGct              <  1:490424/133‑2 (MQ=255)
                                                                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct              <  1:312212/133‑2 (MQ=255)
                                                                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct              <  2:654528/133‑2 (MQ=255)
                                                                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct              >  1:97691/8‑139 (MQ=255)
                                                                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACATTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct              >  1:478801/8‑139 (MQ=255)
                                                                                                                         tagatatgCTTGTATATTTACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg             <  1:169529/134‑3 (MQ=255)
                                                                                                                         tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGcc              <  1:249326/140‑1 (MQ=255)
                                                                                                                          agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GTCGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt            <  1:230962/135‑4 (MQ=255)
                                                                                                                          agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt            <  1:118944/135‑4 (MQ=255)
                                                                                                                          agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt            <  2:25474/135‑4 (MQ=255)
                                                                                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtgatgtt           >  2:484475/5‑135 (MQ=255)
                                                                                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt           >  2:103259/5‑136 (MQ=255)
                                                                                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt           <  1:246185/136‑5 (MQ=255)
                                                                                                                           aCTGCGCTTCTATATTTGCCGATGATATAATAGTAGAGTCGCCTATTTCTCAGGCGTTAATTTAGACGTAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt            <  1:315532/140‑1 (MQ=255)
                                                                                                                            cTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt           >  1:407211/1‑140 (MQ=255)
                                                                                                                            atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg          >  1:602345/4‑135 (MQ=255)
                                                                                                                            atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg          >  1:236127/4‑135 (MQ=255)
                                                                                                                             tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATTAACGAAGAAAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtttc         >  1:554713/3‑134 (MQ=255)
                                                                                                                             tatgCTTGTATATTCACAGAGGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc         <  2:267487/138‑7 (MQ=255)
                                                                                                                              gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTTTCGCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc         <  1:123323/140‑1 (MQ=255)
                                                                                                                              atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGAACCAGTCATCAGTGGCTGTGCTGTTGCg        >  1:336112/2‑140 (MQ=255)
                                                                                                                               tgCTTGTATATTTGCCGATGACAGAATAGTAGAGTCGTCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTTTATAAGGTGATGCTTTTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg        <  2:86822/140‑1 (MQ=255)
                                                                                                                                  ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt    <  1:500520/140‑4 (MQ=255)
                                                                                                                                  ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt    <  2:340199/140‑4 (MQ=255)
                                                                                                                                   tGTATATTCACAAATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta   <  1:59706/140‑5 (MQ=255)
                                                                                                                                    gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat  <  1:652921/140‑3 (MQ=255)
                                                                                                                                     tataTTTGCCGATGAAATAATAGGAGAGTTGCCTATCTCTCAGGCGTCAATTTAGTCGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCCATTGCGtttttt  <  1:261145/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CTTGTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTT  >  CP000730/1929657‑1929929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: