Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,915,579 T→C 6.1% T81T (ACA→ACG metK ← methionine adenosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,915,5790TC 6.1% 215.0 / 4.1 80T81T (ACA→ACGmetKmethionine adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (37/38);  new base C (2/3);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

TTCAATTTCTTCTTCGCTATCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGCTACACGTGCATTTGGATCGTCTT  >  CP000730/1915447‑1915718
                                                                                                                                    |                                                                                                                                           
ttCAATTTCTTCTTCGCTATCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTTTATc                                                                                                                                      >  1:639749/1‑140 (MQ=255)
gtCAATTTCTTCTTCGCTATCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATc                                                                                                                                      <  2:337344/139‑1 (MQ=255)
   aaTTCCTTCTTCGCTATCTTTATCCCGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCAGAACCATATTTTGCTCTTGTGTATCCaa                                                                                                                                   <  1:220044/140‑1 (MQ=255)
     gttcttcttcGCTATCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAAtt                                                                                                                                 <  1:600890/139‑1 (MQ=255)
       tcttcttcGCTATCTTTATCACGATACTCTAATGCTTTGTCCACGCCTTGTGAAATGTCGGGTGATTGTTCATTAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTc                                                                                                                               <  2:236772/140‑1 (MQ=255)
       tcttcttcGCTATCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTTTTCATCAATTGCAGTTAAAATTGTCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTc                                                                                                                               <  1:191843/140‑1 (MQ=255)
         ttcttcGCTATCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCTTAACCATATTTTGCTCTTGTGTATCCAATTTCtt                                                                                                                             >  1:288233/1‑140 (MQ=255)
         ttcttcGCTATCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCtt                                                                                                                             >  1:574814/1‑140 (MQ=255)
            ttcGCTATCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCTGGTGATTGTTCATCAATCGCATTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTaa                                                                                                                          <  2:435040/140‑1 (MQ=255)
             tcacTATCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAAt                                                                                                                         <  2:10560/137‑1 (MQ=255)
             tcGCTGTCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAAt                                                                                                                         <  2:10557/140‑1 (MQ=255)
             tcGCTATCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAACATTGCCATTGTTTCATAATCATAACCATATTTTGCGCTTGTGTATCCAATTCCTTTCAt                                                                                                                         >  2:462341/1‑140 (MQ=255)
             tcGCTATCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAAt                                                                                                                         <  1:199493/140‑1 (MQ=255)
              cGCTATCTTTATCACGATACACTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTAGACAATTTCTTTAAtt                                                                                                                        >  2:271859/1‑140 (MQ=255)
                cTATCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGt                                                                                                                      <  2:281492/140‑1 (MQ=255)
                   tCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTCGTGTATCCAATTTCTTTAATTGTTtc                                                                                                                   <  2:414490/140‑1 (MQ=255)
                     tttATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTtctc                                                                                                                 >  1:281735/1‑140 (MQ=255)
                        aTCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTaa                                                                                                              <  2:175419/140‑1 (MQ=255)
                         tCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTCCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTaac                                                                                                             >  2:104421/1‑140 (MQ=255)
                          caggATACTCGAACGCTGTACCCACGCCTTGTGCAATGTCAGGTGATTGTTCCTCACTCGCACTTAAAACTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTaaca                                                                                                            <  2:431415/137‑1 (MQ=255)
                          cACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTACCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCCATTTCTTTAATTGTTTCTCTaaca                                                                                                            >  1:542165/1‑140 (MQ=255)
                           aCGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTTATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTaacaa                                                                                                           <  1:312305/140‑1 (MQ=255)
                             gATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTCGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACt                                                                                                         >  1:439423/1‑140 (MQ=255)
                             gATACTCTAATGATTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTCGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACt                                                                                                         >  1:470980/1‑140 (MQ=255)
                              aTACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTAACAGAAGCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACtt                                                                                                        >  1:654701/1‑140 (MQ=255)
                               tACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACttt                                                                                                       <  1:64256/140‑1 (MQ=255)
                               caCTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACttt                                                                                                       <  1:2267/139‑1 (MQ=255)
                                 ctctAATGCTTTATCCACGTCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCTATTGTTTCATAATCATAACCATATTTTGCTCGTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCg                                                                                                     <  2:562253/140‑1 (MQ=255)
                                  tctAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCgg                                                                                                    >  1:294417/1‑140 (MQ=255)
                                            tATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACa                                                                                          <  1:567737/140‑1 (MQ=255)
                                                cACGCCTTGAGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATAtg                                                                                      >  1:488258/1‑140 (MQ=255)
                                                 aCGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATAtgt                                                                                     >  1:196715/1‑140 (MQ=255)
                                                   gCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATAtgttg                                                                                   <  1:95435/140‑1 (MQ=255)
                                                    gcTTGAGCAATTTTAGGTGATTGTTCATCAATTGCAGTTAAATTTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCGAACAACTTTCGGAATATCAACATAtgttgt                                                                                  <  1:84440/139‑1 (MQ=255)
                                                    ccTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATAtgttgt                                                                                  <  1:328104/140‑1 (MQ=255)
                                                          gCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTACTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTAGGAATATCAACATATGATGTTGTata                                                                            >  1:83090/1‑138 (MQ=255)
                                                           cAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCGACATATGTTGTTGTAGaa                                                                           >  1:530836/1‑140 (MQ=255)
                                                               gTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATACCAACATATGTTGTTGTAGAAAttt                                                                       >  1:364288/1‑140 (MQ=255)
                                                                  aGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGc                                                                    >  1:52248/1‑140 (MQ=255)
                                                                  aGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACAATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGc                                                                    >  1:192083/1‑140 (MQ=255)
                                                                    gTGATTGCTCATCCATCGCAGATAAAATTGCCATTGTTTCATAAACAGAACCATATGTTGCTCTTGTGGATCCAATTGTTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCg                                                                  >  2:629852/1‑140 (MQ=255)
                                                                        ttGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAATTATAGGAATATAAACATATGTAGTTGTAGAAATTTCGCCGGCGa                                                              >  2:95477/1‑140 (MQ=255)
                                                                        ttGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAAGATCAACATATGTTGTTGTAGAAATTTCGCCGGCGa                                                              <  1:192386/140‑1 (MQ=255)
                                                                           gtcatcaATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCa                                                           <  2:574814/139‑1 (MQ=255)
                                                                               tcaATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGACTATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGc                                                       <  1:519635/140‑1 (MQ=255)
                                                                                caATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGcc                                                      <  2:567664/140‑1 (MQ=255)
                                                                                  aTCGCAGTTAAATTTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTTTGTATCTAATTTCTTTAATTGTTTCTCTAAAAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCAt                                                    <  1:55482/140‑1 (MQ=255)
                                                                                  aTCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCAt                                                    <  1:189138/140‑1 (MQ=255)
                                                                                   tCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTGACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATa                                                   <  2:566107/140‑1 (MQ=255)
                                                                                   tCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATa                                                   >  2:539493/1‑140 (MQ=255)
                                                                                   tCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCCATCAATGCCATa                                                   >  2:623043/1‑140 (MQ=255)
                                                                                      taGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCt                                                <  1:93194/139‑1 (MQ=255)
                                                                                         ttAAAATTGCCATTGTTTCATAATCATAACCTTTTTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTACCAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCtgtt                                             <  2:484261/140‑1 (MQ=255)
                                                                                         ttAAAATTGCCATTGCTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCtgtt                                             >  1:492319/1‑140 (MQ=255)
                                                                                                 gCCATTGTTTCATAATCATAACCATATTTTGCTCTCGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAgt                                     <  2:470980/140‑1 (MQ=255)
                                                                                                 gCCATTGTTTCATAATCATAACCATATTTTGCTCTCGTGTATCCAATTTCTTTAATTCTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAgt                                     <  2:439423/140‑1 (MQ=255)
                                                                                                  ccATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAgtt                                    >  2:153771/1‑140 (MQ=255)
                                                                                                   cATTGTTTCATAATCATAACCATATTTTGCTCTTGAGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCCGCGATCAATGCCATACCTGTTGTAACAgttg                                   <  2:451150/140‑1 (MQ=255)
                                                                                                      tGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGttt                                >  1:520825/1‑140 (MQ=255)
                                                                                                           cataatcataaCCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACaa                           <  2:295524/140‑1 (MQ=255)
                                                                                                            ataatcataaCCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGCTTCTCTAACAACTTACGGAATAACAACATAAGAAGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTAGTAACAGTTGTTTCACAAg                          >  1:79369/1‑140 (MQ=255)
                                                                                                             taatcataaCCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGc                         <  1:665560/140‑1 (MQ=255)
                                                                                                             taatcataaCCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGc                         >  2:423518/1‑140 (MQ=255)
                                                                                                             taatcataaCCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGAAATATCAACAACTATGAACAAAAAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGc                         >  1:316551/1‑140 (MQ=255)
                                                                                                                  ataaCCATTTTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGCTacac                    <  2:239520/140‑1 (MQ=255)
                                                                                                                  ataaCCATATTTTGCTCTTGTGTATCCAATTTCTTTCATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGCTacac                    <  2:64042/140‑1 (MQ=255)
                                                                                                                      ccATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACTTTTGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGCTACACGTGc                <  1:401756/140‑1 (MQ=255)
                                                                                                                       cATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTGGCCGGCGATCAATGCCATACCTGTTGTAAAAGTTGTTTCACACGCTACCCGTGCa               >  1:537772/1‑140 (MQ=255)
                                                                                                                       cATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGCTACACGTGCa               >  2:285914/1‑140 (MQ=255)
                                                                                                                       cATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGCTACACGTGCa               >  1:643207/1‑140 (MQ=255)
                                                                                                                         tatTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGCTACACGTGCAtt             <  1:422984/140‑1 (MQ=255)
                                                                                                                           ttttGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCCCAAGCTACACGTGCATTTg           >  2:50744/1‑140 (MQ=255)
                                                                                                                           ttttGCTCTTGGGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGCTACACGTGCATTTg           <  2:83640/140‑1 (MQ=255)
                                                                                                                            tttGCTCTTGTGTAACCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGCTACACGTGCATTTgg          >  2:452148/1‑140 (MQ=255)
                                                                                                                              tGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGCTACACGTGCATTTGGAt        <  2:154464/140‑1 (MQ=255)
                                                                                                                                 tctTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGCTACACGTGCATTTGGATCGt     >  1:387713/1‑140 (MQ=255)
                                                                                                                                 tctTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTGGTAACAGTTGTTTCACAAGCTACACGTGCATTTGGATCGt     >  2:312024/1‑140 (MQ=255)
                                                                                                                                 tctTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGACGGGGATCAATGCCATACCTGTTGTAACAGTTGTTTCCCACGCTGCCCGTGCGTATGGATCGt     >  2:394711/1‑140 (MQ=255)
                                                                                                                                 tctTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCAGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCCTACCTGTTGTAACAGTTGTTTCACAAGCTACACGTGCATTTGGGTCGt     >  2:239388/1‑140 (MQ=255)
                                                                                                                                 tctTGTGTATCCAATTTATTTAATTGTTTCTCTAACAACTTTCGGGATATAAACATATATTTTTGTAGAAATTTGGCATGTGATCAATGCTATACCTGTTGTAACAGTTGTTTCACAAACTACACGTGCATTTTGATcat     >  1:303740/1‑138 (MQ=255)
                                                                                                                                    tgtgTATCCAATTTCTTTAACTTTTTCTCTAACAACTCTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATCCCTGTTGTAACAGTTGTTTCACAAGCTACACGTGCATTTGGATCGTCtt  <  2:217697/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                           
TTCAATTTCTTCTTCGCTATCTTTATCACGATACTCTAATGCTTTATCCACGCCTTGTGCAATGTCAGGTGATTGTTCATCAATCGCAGTTAAAATTGCCATTGTTTCATAATCATAACCATATTTTGCTCTTGTGTATCCAATTTCTTTAATTGTTTCTCTAACAACTTTCGGAATATCAACATATGTTGTTGTAGAAATTTCGCCGGCGATCAATGCCATACCTGTTGTAACAGTTGTTTCACAAGCTACACGTGCATTTGGATCGTCTT  >  CP000730/1915447‑1915718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: