Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 728,366 G→A 6.5% G50S (GGT→AGT)  USA300HOU_0675 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730728,3660GA 6.5% 186.1 / 2.7 69G50S (GGT→AGT) USA300HOU_0675hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base G (36/28);  new base A (3/2);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01

TTCACTAAATATGGCTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCTAGGTGCTTTAATTATGA  >  CP000730/728228‑728504
                                                                                                                                          |                                                                                                                                          
ttCACTAAATAGGACTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTGAGCTTTTATTGGGCGTCAAGTACCAAAAATTGGTACTGCCTTTGAAGAGACAAATCATGAATCAACATTACAATACGTATTCgg                                                                                                                                           <  2:186084/140‑1 (MQ=255)
 tCACTTAATATGGCTTGTAGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGt                                                                                                                                          <  2:635271/140‑1 (MQ=255)
  cACTAAATATGGCTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTg                                                                                                                                         <  2:256057/140‑1 (MQ=255)
   aCTAAATATGGCTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGATTCAACATTACAATACGTATTCGTTGa                                                                                                                                        >  1:649942/1‑140 (MQ=255)
    cTAAATATGGCTTGTGGGCTTATTTAGGTGATATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTGCAATACGTATTCGGTGaa                                                                                                                                       >  2:572347/1‑140 (MQ=255)
            ggCTTGTGGGCTTATTTAGGTGTTGTTATATCTGGTTTTATTTTATCTTTTATTGGACGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAg                                                                                                                               >  1:342906/1‑140 (MQ=255)
             ttttGTGGGTTTATTTATGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCACGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGt                                                                                                                              <  1:164917/138‑1 (MQ=255)
             gCTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGaaagcgttagt                                                                                                                              >  1:192917/1‑132 (MQ=255)
             gCTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGt                                                                                                                              <  2:95041/140‑1 (MQ=255)
             gCTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGt                                                                                                                              >  2:533809/1‑140 (MQ=255)
              cTTGTGGGCTTATTTGGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTa                                                                                                                             >  1:494694/1‑140 (MQ=255)
              cTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTa                                                                                                                             >  1:662980/1‑140 (MQ=255)
              cTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTa                                                                                                                             >  1:266259/1‑140 (MQ=255)
              cTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTa                                                                                                                             >  1:93418/1‑140 (MQ=255)
              cTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGAGCGACAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTa                                                                                                                             >  1:21491/1‑140 (MQ=255)
                tgtgGGCTTATTTATGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTaaa                                                                                                                           <  2:446221/140‑1 (MQ=255)
                    ggCTTATTTAGTTGTTATTATATCTTGTTTTATTTTAGCTTTTATTGGGCGGCAAGTAGCAAAAATTGGTACTGCTTTTGAAGCGACAAATCATGAATCAACCTTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCt                                                                                                                       <  2:30992/140‑1 (MQ=255)
                     gCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGACGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCtt                                                                                                                      >  2:427161/1‑140 (MQ=255)
                      cTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCttt                                                                                                                     >  1:388789/1‑140 (MQ=255)
                         aTTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGAt                                                                                                                  <  2:483911/140‑1 (MQ=255)
                                       atatCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAAtctt                                                                                                    >  1:640956/1‑140 (MQ=255)
                                       atatCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATAATGAATCAACATTACAATACGTATGCGGGTAAAAGTTTAGTAAAGTCGTTGATTATATTTTATtctt                                                                                                    >  1:177862/1‑140 (MQ=255)
                                        tatCTTGTTTTATTTTAGCTTTTATTGGGTGCCAAGTAGCATAAATTGGTACTGGCTTTGAATCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAAtcttc                                                                                                   <  1:143511/140‑1 (MQ=255)
                                         atCTTGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAAtcttct                                                                                                  <  1:225988/140‑1 (MQ=255)
                                         atCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTTGCTTTTATTGGTTCTTTTTTTGTTTCGACTTGTCTACAAACAATTTTTTGAATCTAATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAAtcttct                                                                                                  <  1:319642/140‑1 (MQ=255)
                                         atCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAAtcttct                                                                                                  >  2:542703/1‑140 (MQ=255)
                                              gTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGCATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGACTTTGATTATATTTTAATcttcttcttc                                                                                             >  2:578842/1‑139 (MQ=255)
                                               ttttattttaGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTAt                                                                                            <  1:99991/140‑1 (MQ=255)
                                                tttattttaGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTATAGTAAAGTCTTTGATTATATTTTAATCTTCTCCTTAtt                                                                                           >  2:339465/1‑140 (MQ=255)
                                                   attttaGCTTTTATTGGGCGCAAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTTTTCTTATTTgt                                                                                        >  2:438936/1‑139 (MQ=255)
                                                       taGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGtata                                                                                    >  1:339112/1‑140 (MQ=255)
                                                        aGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAg                                                                                   >  1:44617/1‑140 (MQ=255)
                                                        aGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGAGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAg                                                                                   >  2:561743/1‑140 (MQ=255)
                                                                    gcgcCAAGTAGCAAAAATTGGTACTCCCTTTTAAGTGACAAACCATGACTCAATATTACAATACGTATCCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGa                                                                       <  2:192917/140‑1 (MQ=255)
                                                                          aGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTCGATTATATTTTAATTTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTg                                                                 >  1:548445/1‑140 (MQ=255)
                                                                           gTAGCAAAAATTGGTATTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTgg                                                                <  2:582498/140‑1 (MQ=255)
                                                                            tAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAATTTAGAAAAATCTTTGATTATATTTTAATCTTCTTCTTATTTCGAAGAGCTTGAACCATGATAGCTGGt                                                               >  1:152212/1‑140 (MQ=255)
                                                                            tAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCAGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGt                                                               >  2:231678/1‑140 (MQ=255)
                                                                            tAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCAGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGt                                                               >  2:231650/1‑140 (MQ=255)
                                                                            tAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCAGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGt                                                               >  1:207526/1‑140 (MQ=255)
                                                                             aGCAAAAATTGGTACTGTCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTTATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTg                                                              <  2:248739/140‑1 (MQ=255)
                                                                                aaaaaTTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAg                                                           >  2:61672/1‑140 (MQ=255)
                                                                                    aTTGTTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGgcgc                                                       <  2:122203/140‑1 (MQ=255)
                                                                                     ttGGTACTGCCTTTGAATCGACAAATCATGAATCAACATTACAAAACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCa                                                      <  2:69803/140‑1 (MQ=255)
                                                                                     ttGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTACTCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCa                                                      <  2:2647/140‑1 (MQ=255)
                                                                                     ttGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCa                                                      <  1:420877/140‑1 (MQ=255)
                                                                                       ggTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGATTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAACTGGTGCAGGCGCAAc                                                    >  2:428650/1‑140 (MQ=255)
                                                                                        gTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACa                                                   >  1:410378/1‑140 (MQ=255)
                                                                                        gTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACa                                                   >  2:552124/1‑140 (MQ=255)
                                                                                         tACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACAt                                                  <  1:610175/140‑1 (MQ=255)
                                                                                         tACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACAt                                                  <  1:533809/140‑1 (MQ=255)
                                                                                          acagCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACAtt                                                 >  2:243918/4‑140 (MQ=255)
                                                                                          aCTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACAtt                                                 >  1:498097/1‑140 (MQ=255)
                                                                                            tGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTg                                               <  2:44617/140‑1 (MQ=255)
                                                                                              ccTTTGAAGCGACAAATCATGAATCAACATTGCAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTgaa                                             >  2:330185/1‑140 (MQ=255)
                                                                                                tttGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTgaaga                                           <  2:172267/140‑1 (MQ=255)
                                                                                                     aGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGtt                                      >  1:247267/1‑140 (MQ=255)
                                                                                                         aCAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCATCATCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGCAGAAAGTTATaa                                  >  1:243973/1‑140 (MQ=255)
                                                                                                              tCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTCTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAATTTATAACATAc                             <  2:172734/140‑1 (MQ=255)
                                                                                                                 tGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTa                          >  1:374740/1‑140 (MQ=255)
                                                                                                                  gAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTag                         >  2:326557/1‑139 (MQ=255)
                                                                                                                       aaCATTACAATACGTATTCGGTTAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGCTATAGCTGTAACCATCATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGc                    <  2:342906/140‑1 (MQ=255)
                                                                                                                        aCATTACAATACGTATTCAGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCt                   <  1:231678/140‑1 (MQ=255)
                                                                                                                        aCATTACAATACGTATTCAGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCt                   <  1:231650/140‑1 (MQ=255)
                                                                                                                         cactACAATACGTACTAGGTGAAAGGTATAGTAAAGTCCTTGATTATATTTTACTCTTATTCTTCTTTGGTATAGATGTAGCCATGATCGCTGGTGTAGATGCAACAATTGAGAAAAGTTATAACATACCTACCTGGCTa                  >  2:186665/4‑140 (MQ=255)
                                                                                                                                 tACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGTAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCTAGGTGCttt          <  2:649942/140‑1 (MQ=255)
                                                                                                                                     tATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACTTACCTACATGGCTAGGTGCTTTAAtt      <  2:592165/140‑1 (MQ=255)
                                                                                                                                      aTTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGTAACATTTGAAGAAAGTTATAACATACCTAGATGGCTGGGAGCTTTAATTa     >  2:7875/1‑140 (MQ=255)
                                                                                                                                        tCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCTAGGTGCTTTAATTATg   <  2:388789/140‑1 (MQ=255)
                                                                                                                                         cGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCTAGGTGCTTTAATTATGa  <  1:81618/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TTCACTAAATATGGCTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCTAGGTGCTTTAATTATGA  >  CP000730/728228‑728504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑