Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,185 G→A 8.4% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1850GA 8.4% 271.1 / 13.4 115intergenic (+243/‑891)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (51/53);  new base A (4/6);  total (55/60)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.16e-01

Only 100 of 123 total aligned reads displayed.

ATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGG  >  CP000730/76048‑76322
                                                                                                                                         |                                                                                                                                         
atatAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                         >  1:100003/1‑140 (MQ=255)
  ataACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGtata                                                                                                                                       >  2:253646/1‑140 (MQ=255)
     aCTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAta                                                                                                                                    >  2:263616/1‑139 (MQ=255)
       tAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAtttt                                                                                                                                  <  1:107539/140‑1 (MQ=255)
                    ccTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGt                                                                                                                     <  1:625803/140‑1 (MQ=255)
                       gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTCACAAAAAGGAGtctt                                                                                                                  >  1:82917/1‑140 (MQ=255)
                       gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAAGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtctt                                                                                                                  >  1:288582/1‑140 (MQ=255)
                       gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGAGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtctt                                                                                                                  >  2:433213/1‑140 (MQ=255)
                        tATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtcttc                                                                                                                 <  1:524716/140‑1 (MQ=255)
                                 gAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCCAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAAc                                                                                                        <  1:457608/140‑1 (MQ=255)
                                  aaCAGCTAACCAAAGTGTTAGTAATTCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACt                                                                                                       <  1:53412/140‑1 (MQ=255)
                                  aaCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACt                                                                                                       <  1:287599/140‑1 (MQ=255)
                                   aCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTa                                                                                                      >  1:220637/1‑140 (MQ=255)
                                    cAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAAAACAAAGGCAATTAATTTAATACTTATAGAAAAATATAGGTACTGTTGACAAGTAGAATCACAAGTCTACTTTTAAACAAAAAGAGACTTCTGtgggaccgct                                                                                                     >  2:627406/1‑131 (MQ=255)
                                       cTAACCAAAGTGTTAGTACTTCAATTAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTCACAAAAAGGCGCCTTCTGTATGAACTCTTTc                                                                                                  >  2:144223/1‑140 (MQ=25)
                                         aaCCAAGGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAg                                                                                                >  2:369187/1‑140 (MQ=255)
                                          aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAACAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAAATTAACAAAAAGGAGTCTACTGGATGAACTATTTCAGa                                                                                               >  1:619973/1‑140 (MQ=37)
                                                         aTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAAc                                                                                >  2:443488/1‑140 (MQ=255)
                                                           ccaaagAACACCAACACAAAGACAACTAAATAAACAAATATAGCAAAACATACGTTCAGTTGCAAAGCTGAATCTATAGTAAAAAAAAAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACaa                                                                              <  2:278716/135‑1 (MQ=9)
                                                               ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGTACTATTTCAGATATAAACAATTTAACAAGGAt                                                                          <  1:477663/140‑1 (MQ=255)
                                                               ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAt                                                                          >  1:414058/1‑140 (MQ=255)
                                                               ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAt                                                                          >  1:261732/1‑140 (MQ=255)
                                                                        acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACt                                                                 <  1:409394/140‑1 (MQ=255)
                                                                         cacaAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTTCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                                <  1:147998/140‑1 (MQ=255)
                                                                           caAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTa                                                              <  1:360518/140‑1 (MQ=255)
                                                                            aaaGGAAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTCAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAg                                                             <  2:365173/140‑1 (MQ=255)
                                                                             aaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAGTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGc                                                            <  1:569821/140‑1 (MQ=255)
                                                                              aGGCAATTAATTTAATATTTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTAGAGTATAAATTTAACAAAAAGTATTCTTCTGTATTAACTATTTCAGTTATAAACAATTTAACAAGGATGTTATCCCTGTAGcc                                                           <  2:432648/140‑1 (MQ=18)
                                                                              aGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                           <  2:30639/140‑1 (MQ=255)
                                                                                  aattaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATATTTTTAACAAAAAGGAGTCTTCTGTAGGAACTATTTCCGATATAAACAATGTAACAAGGATGTTATCACTGTAGCCGTTg                                                       <  1:321524/140‑1 (MQ=255)
                                                                                   attaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTgg                                                      <  2:599161/140‑1 (MQ=255)
                                                                                     taatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTCTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGct                                                    <  2:510042/140‑1 (MQ=255)
                                                                                     taatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGct                                                    <  1:562120/140‑1 (MQ=255)
                                                                                     taatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGAAGCCGTTGGct                                                    >  1:205483/1‑140 (MQ=255)
                                                                                         ttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctacta                                                >  1:368468/1‑140 (MQ=255)
                                                                                         ttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctacta                                                >  2:615768/1‑140 (MQ=255)
                                                                                         ttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctacta                                                >  1:368652/1‑140 (MQ=255)
                                                                                            ataatTATAGCAAAATATAGTTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCt                                             <  1:33441/140‑1 (MQ=255)
                                                                                            ataatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCt                                             <  1:253646/140‑1 (MQ=255)
                                                                                                    aGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATg                                     <  2:100003/140‑1 (MQ=255)
                                                                                                    aGCAAAATATAGGTTCTGTGGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCGGTTGGCTACTATCTAAGATATg                                     <  2:486527/140‑1 (MQ=255)
                                                                                                      cAAAATATGAGTTCTGTTTCCAAGTTTAATCTATAGAAGAATTTTACCAAAAAGGAGGCTTCTGTAGGAACTATTTCAGATATAAACATTTTAACAAGGATGTTATCACTGTAGCCCTTGGCTACTATCTAAGATATGCa                                   <  2:189982/140‑1 (MQ=11)
                                                                                                       gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAt                                  >  1:504351/8‑140 (MQ=11)
                                                                                                       aaaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGGCGTTGGCTACTATCTAAGATATGCAt                                  >  2:580203/1‑140 (MQ=255)
                                                                                                       aaaaTATAAGTTTTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                  <  2:255157/140‑1 (MQ=255)
                                                                                                         ttaggaGGTTCTGTTACAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                                >  1:495713/6‑140 (MQ=1)
                                                                                                         ttagaaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGGTATAAAAAATTGAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                                <  1:247719/135‑1 (MQ=11)
                                                                                                         aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                                <  1:562195/135‑1 (MQ=2)
                                                                                                         aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                                <  2:253548/135‑1 (MQ=255)
                                                                                                         aaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTGAACAAGGATGTTATCACTGTAGCCGATAGGTACTATCTAAGATATGCATTg                                <  2:473249/140‑1 (MQ=255)
                                                                                                          aatgaGGTTCTGTTGTAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCGTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                               <  2:55178/136‑1 (MQ=11)
                                                                                                           tataCGTTCTGTTGCAAAGTTGAATCTATAGTAAAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                              <  2:145586/140‑1 (MQ=11)
                                                                                                           atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                              <  1:489412/137‑1 (MQ=2)
                                                                                                            tgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTCGCCGCTGGCGACTATCTAAGATATGCATTGAGt                             <  1:659460/138‑1 (MQ=11)
                                                                                                            tgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                             <  2:476766/138‑1 (MQ=9)
                                                                                                            ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGt                             >  2:586013/1‑140 (MQ=39)
                                                                                                            ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGAAATATTATGTATGAAATATTTAAGATATAAAAAATTTAAAAAGGATGTTATCACTGTAGCCGTTGGATACTATCTAAGATATGCATTGAGt                             >  1:188018/1‑140 (MQ=11)
                                                                                                             taGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGCACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGATACTATCTAAGCTATGCATTGAGtt                            >  1:467033/1‑140 (MQ=255)
                                                                                                             taGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGtt                            <  1:542183/140‑1 (MQ=37)
                                                                                                             gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGtt                            <  1:580860/139‑1 (MQ=2)
                                                                                                             gaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                            <  2:119271/139‑3 (MQ=9)
                                                                                                              acgTTCTGTTGCAAAGTTGAATGTATAATATAATTTTAACAAAAAGGATTCTTTTTTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTa                           <  1:514460/138‑1 (MQ=11)
                                                                                                              aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                           >  2:64898/1‑140 (MQ=2)
                                                                                                              aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAttaggcta                           >  2:512151/1‑134 (MQ=1)
                                                                                                               ggatCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAGACAGGCGGCTCCTGTATGAACTATTTCAGATCTAAACAATTTAACAAGGACGTTATCACCATAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTAt                          >  2:246447/4‑140 (MQ=11)
                                                                                                                gTTCTGTTGCAAAGTTGAATATATAGTATAATTTTAACAAAAAGGAGTCTACAGTATGAACTATTTCAGATATAAACAATTGAACAAGAATGTTATCACTGTAGCAGTTGGCTACTAACTAAGATATGCATTGAGTAATc                         >  2:71773/1‑140 (MQ=11)
                                                                                                                  tCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                       <  1:580345/140‑1 (MQ=35)
                                                                                                                  tCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCCTCTGTATTAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                       <  1:492048/140‑1 (MQ=255)
                                                                                                                   cTGTTGCAAAGTTGAATCTATAGTAAAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTTAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTg                      <  1:634008/140‑1 (MQ=25)
                                                                                                                    tGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGa                     <  2:384727/140‑1 (MQ=37)
                                                                                                                     gTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGAATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGat                    >  1:351155/1‑140 (MQ=25)
                                                                                                                      ttGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATATAAGATATGCATTGAGCTATCGTGata                   >  2:644102/1‑140 (MQ=255)
                                                                                                                        gCAAAGTTGAATCTATAGTATAATTTTAACAAAACGGAGTCTCCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCCCTGTAGCCGTTGACTACTATCTAAGATATGCATGGGGTTAACGTGatatt                 >  2:516570/1‑139 (MQ=11)
                                                                                                                        gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatata                 >  2:290624/1‑140 (MQ=34)
                                                                                                                        gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatata                 >  2:493886/1‑140 (MQ=35)
                                                                                                                         cAAAGTTGAATATATAGTAAAAAAAAAAAAAAGAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat                <  2:373292/140‑1 (MQ=11)
                                                                                                                          aaattttcattcataGTATAAATATAACAAAAAGGATTCCTCTGTTTGAACTAGTCCAGAAATAAACAATTTATCAAGAATGTTATCACTGTAGCTGTTGTCTATTATCTAAGATATGCATTGAGTTATCGTGATATATc               <  2:325799/128‑1 (MQ=11)
                                                                                                                          aaaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAATTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc               >  2:492407/1‑140 (MQ=25)
                                                                                                                            aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg             <  1:586013/140‑1 (MQ=35)
                                                                                                                              ttGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaa           >  2:278563/1‑140 (MQ=37)
                                                                                                                               tGAAACAATACTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaaa          <  2:162442/140‑1 (MQ=16)
                                                                                                                                 aaTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAata        <  1:638909/140‑1 (MQ=35)
                                                                                                                                  atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAatat       >  2:402950/4‑140 (MQ=0)
                                                                                                                                  atttataGTATAATTTTAACAAAAAGGAGTTTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAatat       <  1:122922/137‑1 (MQ=2)
                                                                                                                                  atttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTCTTTCAGATATAAACAATTTAAAAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAattt       >  2:622724/4‑138 (MQ=1)
                                                                                                                                  atttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAatat       >  1:336888/4‑140 (MQ=1)
                                                                                                                                   tttataatataaTTTTAACAAAAAGGCGTCTTCTTTTTGAACTATTTCAGATATAAACAATTCAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt      <  1:450668/138‑1 (MQ=11)
                                                                                                                                   tttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt      <  2:218442/138‑1 (MQ=9)
                                                                                                                                   tttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGATACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATAtt      >  1:229767/3‑140 (MQ=2)
                                                                                                                                   tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg             <  1:84463/133‑1 (MQ=34)
                                                                                                                                   tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg             >  2:84463/1‑133 (MQ=33)
                                                                                                                                   tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATAtt      >  1:574061/1‑140 (MQ=33)
                                                                                                                                     tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa    <  1:102959/140‑1 (MQ=16)
                                                                                                                                     tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAAAAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGCGCTATCGTGATATATCTGAAATATTaa    >  2:175440/1‑140 (MQ=255)
                                                                                                                                     tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTGCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATAGGCATTGAGCTATCGTGATATATCTGAAATATTaa    >  2:167928/1‑140 (MQ=255)
                                                                                                                                      ataatataatTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGTCGTTGGCTACTATCTTAGATATGCATTAAGATATAGTGATAGATCttgattagtaag   >  1:93292/1‑129 (MQ=11)
                                                                                                                                      ataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAg   <  2:461788/140‑1 (MQ=18)
                                                                                                                                       taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATACACAATTTAACAAGGATGTTATCACTGTAGCTGTTGAATACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATGTTAAgg  >  1:480075/1‑140 (MQ=11)
                                                                                                                                       taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACACTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTACgg  >  2:63608/1‑140 (MQ=12)
                                                                                                                                       taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgg  >  1:365121/1‑140 (MQ=17)
                                                                                                                                         |                                                                                                                                         
ATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGG  >  CP000730/76048‑76322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: