BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,315	G→A	55.0%	Y79Y (TAC→TAT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,315	0	G	A	55.0%	74.0 / inf	594	Y79Y (TAC→TAT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (111/147); new base A (143/176); total (266/328)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 601 total aligned reads displayed.

TACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATT  >  CP000730/36176‑36453

tACCAACTTGTTTAATTAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTTGAGTTTGACTCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTTCTTTTATTTTGATg                                                                                                                                            <  2:304001/140‑1 (MQ=11)

  ccAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTa                                                                                                                                          <  2:462669/140‑1 (MQ=33)

       ttGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtct                                                                                                                                     <  1:278540/140‑1 (MQ=12)

         ttttAATGAGACGTTTGATAAACGCATATGCTTAACGATTATCTCGTTCCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAACAGCTCCATTTTCCTTTTATTTTGATGTACGTCtca                                                                                                                                   <  2:316834/139‑1 (MQ=255)

         gTTTAATGAGCCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtca                                                                                                                                   <  2:169621/140‑1 (MQ=255)

          tttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                                                                  <  2:582384/140‑1 (MQ=32)

           ctaattggaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCATCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcatc                                                                                                                                 <  2:360109/133‑1 (MQ=11)

            tAATGAGCAGTTTGACAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcatca                                                                                                                                <  1:414336/140‑1 (MQ=12)

            tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcatca                                                                                                                                >  2:551407/1‑140 (MQ=17)

            tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTGTACGTCtcatca                                                                                                                                >  1:73896/1‑140 (MQ=17)

            tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGATTACGCAACCAAATATCTAATATCTGTCACTCTGCATCAATGGTCCGATATAACTAACGCCCTCTTCCTTTTATTTTGTTGTCTGTCtcctca                                                                                                                                >  2:516381/1‑140 (MQ=11)

             aaTGAGGCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAGATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAAGAGCTCCATTTTCCTTTTATTTTGATATTCGTCtcatcac                                                                                                                               >  2:178820/1‑139 (MQ=11)

             aaTGAGACGTTTGATAAACGCATATGCTGGATGATTATCTCGTTGCTTACGCCACCAAATAACTAATGTCTGACGCTCTGCATCAATGGCACGATATAGCTAGCCCCCTTTTCCTTTTATGTTTATGTACGTCCCACCaa                                                                                                                               >  2:184666/1‑140 (MQ=11)

               tGAGACGTTTGATAAACTCATATGTTGTATGATTATCTTTTTGCTTACGCAACCAATTATCTAATGTATGTTCCTCTGTATCAATGGTATGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                                                             <  1:308175/140‑1 (MQ=11)

                  gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATACCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGc                                                                                                                          <  1:594796/140‑1 (MQ=17)

                     gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGACACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGGCTCATCAATACTCCaa                                                                                                                       >  1:48495/1‑139 (MQ=12)

                      tttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCAATTTCCTTTTATTTTGAATTACGTCTCATCAATACGCCAtt                                                                                                                      >  1:240534/1‑140 (MQ=12)

                         gATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGt                                                                                                                   <  2:410863/140‑1 (MQ=12)

                         gATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGt                                                                                                                   <  2:380724/140‑1 (MQ=32)

                           tAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaa                                                                                                                 <  2:161965/140‑1 (MQ=12)

                           tAAACGCATATGCTGAATGATTATCTCGGTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaa                                                                                                                 <  2:402433/140‑1 (MQ=12)

                            aaaCGCATAGGCTTAATGATTATCTCGTTTCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaat                                                                                                                <  2:433582/140‑1 (MQ=12)

                              aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATTTAATGTATGTACCTCTGTATCAATGGCACGATATAAATAGCCCCATTTTCCTTTTATTTTGATATACCTCTCATCAATACGCCATTTGtactaa                                                                                                              >  2:54145/1‑140 (MQ=11)

                                  atatGCTGAATGATTATCTCGTTGCTTACGCAACCACATATCTAATGTATGTCCCTCTGCATCAATGGCACGCTATAAATAGCTCCATAGTCCTTTTATTTTGATGTACGTCTCATCAATCAGCCATTTGtaatagtctt                                                                                                          >  2:137036/1‑135 (MQ=11)

                                   tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGAttt                                                                                                         >  1:103655/1‑140 (MQ=25)

                                     tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCttttt                                                                                                       >  1:442835/1‑140 (MQ=35)

                                      gCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                                                                      >  1:429130/1‑140 (MQ=255)

                                       cTGAATGATTATCTCGTTGCTTACGCAACCAAACATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTCTTTTGATATACGTCTCATCAATACGCCACTTGTAATAAGCTTTTTTa                                                                                                     <  2:427404/140‑1 (MQ=11)

                                        tGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATCAAGAAAAACGACTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                                                                    >  1:547996/1‑140 (MQ=11)

                                        tGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                                                                    <  1:524430/140‑1 (MQ=38)

                                         taaTGATTATCTCTTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                                                                   <  2:290341/139‑1 (MQ=255)

                                          aaTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGc                                                                                                  <  1:618465/140‑1 (MQ=255)

                                          aaTGAATAGCTCGTTTCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGACGTACGTCTCATCAATACGCCATTTGTAATAAGCTttattaact                                                                                                  <  2:6540/140‑4 (MQ=11)

                                           aTGACTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCt                                                                                                 <  2:246376/140‑1 (MQ=255)

                                             tattattCCGTTCCTTACGCAACCAAATATCTAATGTATGTCCCTCCGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttt                                                                                               <  2:443150/139‑1 (MQ=255)

                                              aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtt                                                                                                          >  1:459810/1‑128 (MQ=35)

                                                tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttc                                                                                            >  1:587180/1‑140 (MQ=40)

                                                 aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATATATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                           <  1:382538/140‑1 (MQ=255)

                                                  tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                          <  1:50257/140‑1 (MQ=40)

                                                   ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                         <  1:225852/140‑1 (MQ=255)

                                                    tcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCAATCTGTAATAATCTTTTTTATGCTTTTTCTTcc                                                                                        <  1:659758/140‑1 (MQ=14)

                                                      gTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCCATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACATCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTGCaa                                                                                      >  2:532718/1‑140 (MQ=14)

                                                      gTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaa                                                                                      <  2:324657/140‑1 (MQ=40)

                                                             aCGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCACCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTCAt                                                                               >  2:12591/1‑140 (MQ=14)

                                                                  aCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaa                                                                          <  2:640650/140‑1 (MQ=255)

                                                                  aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAACACCATTTTGCTTTTATTTTGATGTACGTCTCATCAAGACGCCAGATGTAATGAACTCTTTTATGCTTTGTCATCCAAATTTGAtctaaa                                                                          >  2:367093/1‑135 (MQ=11)

                                                                    cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTCTTAGAAGAATTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                        >  1:513952/1‑140 (MQ=11)

                                                                      aaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGCCTCATCAATACGCCATTTGTATTAAACGTTTTTATCGTTTTTCTTCCAAATTTGATGTAAACTTg                                                                      >  1:561064/1‑140 (MQ=11)

                                                                        tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATATGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTggg                                                                    >  2:151190/1‑140 (MQ=255)

                                                                         atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCTATTTGTAATAAGCTTTTTTATGATTTTTCTGCCAAATTTGATATAAAATTgggg                                                                   <  1:11361/140‑1 (MQ=11)

                                                                          tCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                                                                  <  1:365747/140‑1 (MQ=37)

                                                                            tAATGTATGTCCCTCTGCATCAATGGCCCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCGTCCAAATTTGATATAAAATTGGGGCat                                                                <  2:25304/140‑1 (MQ=255)

                                                                            tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATGTAAAATTGGGGCat                                                                <  2:452682/140‑1 (MQ=255)

                                                                            tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                >  2:614358/1‑140 (MQ=255)

                                                                            tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                <  1:565651/140‑1 (MQ=37)

                                                                            tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCACAATTTTCCTTTTAGTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAACATTTTTTTGCTTTTTCTTGAAAATTTGATTTAAAATTAGGGcct                                                                >  2:238423/1‑138 (MQ=11)

                                                                               tgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTTCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGATTTTTTTTCCAAATTTGATATAAAATTGGGGCatcta                                                             >  1:143622/1‑137 (MQ=11)

                                                                                   tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAACTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTg                                                         >  1:534056/1‑140 (MQ=255)

                                                                                   tgtCACTCTGCATCAATGGCACGATATAAATAGGAACATTGTCCTTTTATTTTGATGTCCGTATCATCAATAAGCGATTTGTAAGAAGGTTTTTTATGCTTTTTCTTCCAAATAGAAGCTAAAATTGCGGCGTGTTCTTg                                                         >  2:213389/1‑140 (MQ=2)

                                                                                     tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                       >  1:260548/1‑140 (MQ=255)

                                                                                     tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                       >  2:478979/1‑140 (MQ=21)

                                                                                          ctgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCaa                                                  <  2:629464/136‑1 (MQ=255)

                                                                                          ctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTCTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAATCCaa                                                  >  1:622724/1‑140 (MQ=255)

                                                                                             taTCAATGGCACGATATAAATCGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                               <  2:101319/139‑1 (MQ=255)

                                                                                             cATCCATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTAATGTACGTCTCATCAATACGACATTTGTAATACGCTCTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGACATATTCTTCAATCCAACgg                                               >  2:199471/1‑140 (MQ=11)

                                                                                                    ggCACGATATAAATAGCTCCAATTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTGCAAATTTGATATAAGATTGGGGCATATTCTTGAACTCAACGGTAGATCg                                        >  1:341601/1‑140 (MQ=11)

                                                                                                       aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTAATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTg                                     >  1:24561/1‑140 (MQ=11)

                                                                                                        cGATATAAATAGCTCCTTTCTCCTTCTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                    <  1:647661/140‑1 (MQ=255)

                                                                                                        cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                    >  1:603510/1‑140 (MQ=25)

                                                                                                        cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTAATGCTTTTTCATCCAAATTTGCTATAAAATTGGGGCATATTATTGAACCCAACGGTAGATCGTTGa                                    >  1:286570/1‑140 (MQ=11)

                                                                                                         gATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                   >  1:638729/1‑140 (MQ=21)

                                                                                                          atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTGGATTGTTGAat                                  >  1:606692/1‑140 (MQ=11)

                                                                                                            ttaACTAGCTCCATTTTCCGTTTATTTTCATATCCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                <  2:62489/139‑1 (MQ=11)

                                                                                                            ataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACAGTCGACCGatgaatga                                >  1:297821/1‑140 (MQ=255)

                                                                                                               aaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtaatga                             >  1:329413/1‑140 (MQ=21)

                                                                                                                  atcTCCCTTTTTCTTTTATTTGGATTGTCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTCTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACg                          <  2:340089/138‑1 (MQ=11)

                                                                                                                  aGCTGCCTTGTCCTTTTCTTTTGGTCTACGTCGCATCAATATGCCATGTGTAATCAGCTTTTTTCTGCTTTTTCTTCCAAAGTTGATGTAAAAGTGGGGCATATTCTTGACCCCAACGGTAGACCGTTGAATGATGATCg                          <  2:80158/140‑1 (MQ=11)

                                                                                                                  aGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACg                          >  1:128669/1‑140 (MQ=40)

                                                                                                                  aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGACTGATGAACg                          >  2:665908/1‑140 (MQ=255)

                                                                                                                      ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                      <  1:391262/140‑1 (MQ=255)

                                                                                                                      ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                      >  2:385685/1‑140 (MQ=255)

                                                                                                                       cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTac                     <  1:357159/140‑1 (MQ=25)

                                                                                                                        aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAAGGTAGATCGTTGAATGATGAACGTTTaca                    >  1:180835/1‑140 (MQ=255)

                                                                                                                         ttttCCTTTTATTTTGACGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                   <  1:57991/140‑1 (MQ=21)

                                                                                                                           ttCCTTTTATTTTGATATACGTCTCATCAATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                 <  1:314792/140‑1 (MQ=255)

                                                                                                                           ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                 >  1:251448/1‑140 (MQ=255)

                                                                                                                            tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                <  2:493588/140‑1 (MQ=40)

                                                                                                                            tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                <  2:505603/140‑1 (MQ=255)

                                                                                                                             ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg               <  2:576951/140‑1 (MQ=40)

                                                                                                                             ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg               >  2:602526/1‑140 (MQ=255)

                                                                                                                             ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTATTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg               >  1:215115/1‑140 (MQ=255)

                                                                                                                              cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt              <  2:272189/140‑1 (MQ=255)

                                                                                                                               ttttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt             >  2:119017/1‑140 (MQ=25)

                                                                                                                                 ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTcc           >  2:658540/1‑140 (MQ=21)

                                                                                                                                  ttttttGGTATACGACTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctc          <  1:47170/138‑3 (MQ=11)

                                                                                                                                  tATTTGGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTGGGCATATTCTTGAACCCAACGGTAGACCGGTGAATGATGAACGTTTACACCACGTTccc          <  1:336495/140‑1 (MQ=255)

                                                                                                                                    ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGTATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt        <  1:156159/140‑1 (MQ=11)

                                                                                                                                    tttcGATATACGCCTAATCAATACGCCATTTCTAATAAGCTTTTTTATGCTTTTACTTCCAAATTTGATATAAAATTGTGGCATATTCTTGAACCCAACGGGAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt        <  1:203312/140‑1 (MQ=11)

                                                                                                                                       tGATATACGTCTCATCAATACGCCATTTGTAATAAGTTTTTTTTTGTTTTTTTTTCCAAATTTGTTATAAAATTGAGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat     <  1:92013/140‑1 (MQ=11)

                                                                                                                                          tGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAtt  >  2:631441/1‑140 (MQ=21)

TACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATT  >  CP000730/36176‑36453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`