| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,306,533 | A→G | 13.8% | P368P (CCT→CCC) | lmrS ← | hypothetical membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,306,533 | 0 | A | G | 13.8% | 66.1 / 6.0 | 29 | P368P (CCT→CCC) | lmrS | hypothetical membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (15/10); new base G (2/2); total (17/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01 | |||||||||||
GTATATTAACTGCGATATACTAAGTGCCCCAATAACAAAACGAGTTATATCATTTTGTACACCTTCAATTAATATAGAAGGTAAAAACATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATTACTACAGGCAAAATAACAAACCACATATCAA > CP000730/2306398‑2306666 | gTATATTAACTGCGATATACTAAGTGCCCCAATAACAAAACGAGTTATATCATTTTGTACACCTTCAATTAATATAGAAGGTAAAAACATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCGGGTAt > 2:254149/1‑141 (MQ=255)gTATATTAACTGCGATATACTAAGTGCCCCAATAACAAAACGAGTTATATCATTTTGTACACCTTCAATTAATATAGAAGGTAAAAACATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCGGGTAt > 2:254102/1‑141 (MQ=255) atatACTAAGTGCCCCAATAACAAAACGAGTTATATCATTTTGTACACCTTCAATTAATATAGAAGGTAAAAACATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTa < 2:8444/141‑1 (MQ=255) aCTAAGTGCCCCAATAACAAAACGAGTTATATCATTTTGTACACCTTCAATTAATAGAGAAGGTAAAAGCAGATCGGCAAAACCGATAATAATCGGTGGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTaaaaa > 1:158707/1‑141 (MQ=255) aaTAACAAAACGAGTTATATCATTTTGTACACCTTCAATTAATATAGAAGGTAAAAACATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAgg < 2:87575/141‑1 (MQ=255) aTTTTGTACACCTTCAATTAATATAGAAGGTAAAAACATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACaaaaaa > 2:244555/1‑141 (MQ=255) acCTTCAATTAATATAGAAGGTAAAAACATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTa < 1:242436/141‑1 (MQ=255) acCTTCAATTAATATAGAAGGTAAAAACATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTa < 1:179550/141‑1 (MQ=255) cAATTAATATAGAAGGTAAAAACATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCg < 2:158707/141‑1 (MQ=255) atatAGAAGGTAAAAACATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCaataa > 2:241307/1‑141 (MQ=255) taGAAGGTAAAAACATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATgg > 1:141994/1‑141 (MQ=255) aaGAAGGTAAAAACATATCGGAAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATAAACTCTAAAAATGGAACAAAAGGTTTAAAAAAAAAAAAAAAAAAGGGCGTGTAGTTCGCAATAATgg < 2:126943/140‑1 (MQ=255) aGAAGGTAAAAACATATCGGCAAAACCGATAATAATCGATTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGt > 2:167341/1‑141 (MQ=255) aaGGTAAAAACATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAg > 2:126349/1‑141 (MQ=255) aCATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTAc > 2:84643/1‑141 (MQ=255) aatCGGCAAAACCCATAATAATCGGTTGAAAAGCCTGTGCCGCTTCAAGTATTTTCAATAACCCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCaa < 1:102654/140‑1 (MQ=255) aaaaCCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCAt > 2:74242/1‑141 (MQ=255) aaCCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATta > 2:209837/1‑141 (MQ=255) aCCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATtac > 2:47862/1‑141 (MQ=255) ataatCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATTACTACAGGc > 1:155134/1‑141 (MQ=255) ataatCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATTACTACAGGc > 2:55925/1‑141 (MQ=255) taatCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATTACTACAGGCa > 2:82778/1‑141 (MQ=255) ttGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATTACTACAGGCAAAATAAc > 2:212676/1‑141 (MQ=255) agaAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATTACTACAGGCAAAATAACaaa < 2:262686/141‑1 (MQ=255) aGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATTACTACAGGCAAAATAACAAACca < 2:98259/141‑1 (MQ=255) cTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATTACTACAGGCAAAATAACAAACCACat < 1:2059/141‑1 (MQ=255) tgCCGCTTCGGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATTACTACAGGCAAAATAACAAACCACATATc < 1:254149/141‑1 (MQ=255) tgCCGCTTCGGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATTACTACAGGCAAAATAACAAACCACATATc < 1:254102/141‑1 (MQ=255) ccGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATTACTACAGGCAAAATAACAAACCACATATCaa > 2:235368/1‑141 (MQ=255) | GTATATTAACTGCGATATACTAAGTGCCCCAATAACAAAACGAGTTATATCATTTTGTACACCTTCAATTAATATAGAAGGTAAAAACATATCGGCAAAACCGATAATAATCGTTTGAGAAGCCTGTGCCGCTTCAGGTATTTGCATTAACTCTAAAAATGGAACAAAAGGTTTACCCAATATGACAAAAAAGGGCGTGTAGTTCGCAATAATGGTAGCAATAGTACCAATACTCATTACTACAGGCAAAATAACAAACCACATATCAA > CP000730/2306398‑2306666 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |