| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,187,071 | C→T | 100% | R331K (AGA→AAA) | rsbU ← | sigma factor B regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,187,071 | 0 | C | T | 100.0% | 59.9 / NA | 18 | R331K (AGA→AAA) | rsbU | sigma factor B regulator |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (7/11); total (7/11) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
ATTCATTTCATTACACTCCTACTTATTAAAAATATTTATCTTCATTTTAAGCGCTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTCTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCATATGTTTATGTTTTTTAATATATTCTAAAAGTTTTTGTTT > CP000730/2186937‑2187207 | aTTCATTTCATTACACTCCTACTTATTAAAAATATTTATCTTCATTTTAAGCGCTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTta < 2:260096/141‑1 (MQ=255) tCCTACTTATTAAAAATATTTATCTTCATTTTAAGCGCTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCat < 2:11310/141‑1 (MQ=255) tCCTACTTATTAAAAATATTTATCTTCATTTTAAGCGCTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCat < 2:176005/141‑1 (MQ=255) aTCTTCATTTTAAGCGCTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGtt < 1:200226/141‑1 (MQ=255) aTCTTCATTTTAAGCGCTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGtt < 1:262319/141‑1 (MQ=255) aTTTTAAGCGCTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAg > 1:218321/1‑141 (MQ=255) aGCGCTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTaa < 2:236207/141‑1 (MQ=255) gcgcTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTaaa > 1:63773/1‑141 (MQ=255) gcgcTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTaaa > 1:149503/1‑141 (MQ=255) atataCCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTgg > 1:197723/1‑141 (MQ=255) ataatTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATTAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCAtatgtttat > 2:155723/1‑141 (MQ=255) ataatTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGTTAATTTGGACAATATCTTGTGGGTTCAtgtgtttat > 2:44090/1‑141 (MQ=255) ataatTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCAtatgtttat > 1:94392/1‑141 (MQ=255) aatTTCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCAtatgtttatgt < 1:16991/141‑1 (MQ=255) tCTAATCTTCTTTTTTAAATTAATTTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCATATGTTTATGttttt < 1:207613/141‑1 (MQ=255) attaattTACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCATATGTTTATGTTTTTTAATATATTCTAAAAGtt < 2:218321/141‑1 (MQ=255) tACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCATATGTTTATGTTTTTTAATATATTCTAAAAGTTTTTGttt < 2:149503/141‑1 (MQ=255) tACTTTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCATATGTTTATGTTTTTTAATATATTCTAAAAGTTTTTGttt < 2:63773/141‑1 (MQ=255) | ATTCATTTCATTACACTCCTACTTATTAAAAATATTTATCTTCATTTTAAGCGCTGTATCCACCATATTTATATTTCAAATTATTATATACCCATCGAAATAATTTCTAATCTTCTTTTTTAAATTAATTTACTCTTTTTATAATCAAAATAGTCATATCATCTTTTTTATTTGGGTTTTGAAGCTTTAAAATTGCTTCATAGATAATTTGAACAATATCTTGTGGGTGCATATGTTTATGTTTTTTAATATATTCTAAAAGTTTTTGTTT > CP000730/2186937‑2187207 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |