| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,373,835 | C→T | 100% | intergenic (‑60/+53) | pbuG ← / ← topB | NCS2 family nucleobase:cation symporter‑2/DNA topoisomerase TopB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,373,835 | 0 | C | T | 100.0% | 23.1 / NA | 8 | intergenic (‑60/+53) | pbuG/topB | NCS2 family nucleobase:cation symporter‑2/DNA topoisomerase TopB |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (3/5); total (3/5) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGATGCTCCTTTTTTTAAAATACCTTAATAGTTTAATATCATTCTTAATAAAAAACAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGATTATTGTCTAAACCTTCATTTTTATTCATATACTTTTTCATTTC > CP000730/2373706‑2373968 | ttGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGATGCTCCTTTTTTTAAAATACCTTACTAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAg > 1:86811/1‑141 (MQ=255) gTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGATGCTCCTTTTTTTAAAATACCTTAATAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGt < 2:50367/141‑1 (MQ=255) tCACGAATGGATGCTCCTTTTTTTAAAATACCTTAATAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCa > 2:36771/1‑141 (MQ=255) ccTTTTTTTAAAATACCTTAATAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGatta > 2:114154/1‑141 (MQ=255) ttttAAAATACCTTAATAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGATTATTGTc < 2:86811/141‑1 (MQ=255) tACCTTAATAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGATTATTGTCTAAACCtt < 2:33727/141‑1 (MQ=255) aaTAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGATTATTGTCTAAACCTTCAtttt < 2:46836/141‑1 (MQ=255) taaAAAATAATACCAAAAGCGAGCTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGATTATTGTCTAAACCTTCATGTTTATTCATATACTTTTTCATTTc < 2:3412/141‑1 (MQ=255) | TTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGATGCTCCTTTTTTTAAAATACCTTAATAGTTTAATATCATTCTTAATAAAAAACAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGATTATTGTCTAAACCTTCATTTTTATTCATATACTTTTTCATTTC > CP000730/2373706‑2373968 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |