Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,366,219	G→A	11.7%	A46V (GCA→GTA)	rplN ←	ribosomal protein L14

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,366,219	0	G	A	11.7%	93.6 / 3.3	34	A46V (GCA→GTA)	rplN	ribosomal protein L14
Reads supporting (aligned to +/- strand):  ref base G (6/24);  new base A (2/2);  total (8/26)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

CCTTTGTCATCACGGATGATAACACATGCATTTTCATCAAATTTGATGTATGAACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACTTTGATTGTAAGAACTTCACGAGCACCAGAGTTGTCTGCTACTTTCAAGCGTGTTTCTTGTTGGATCA  >  CP000730/2366081‑2366354                                                                                                                                           |                                                                                                                                        ccTTTGTCATCACGGATGATAACACATGCATTTTCATCAAATTTGATGTATGAACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCa                                                                                                                                       >  1:105850/1‑141 (MQ=255) ccTTTGTCATCACGGATGATAACACATGCATTTTCATCAAATTTGATGTATGAACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCa                                                                                                                                       <  2:252748/141‑1 (MQ=255)  cTTTGTCATCACGGATGATAACACATGCATTTTCATCAAATTTGATGTATGAACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCAt                                                                                                                                      <  1:258367/141‑1 (MQ=255)        catcaCGGATGATAACACATGCATTTTCATCAAATTTGATGTATGAACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTaa                                                                                                                                >  1:153350/1‑141 (MQ=255)        catcaCGGATGATAACACATGCATTTTCATCAAATTTGATGTATGAACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTACATTTTTaa                                                                                                                                >  1:172751/1‑141 (MQ=255)        catcaCGGATGATAACACATGCATTTTCATCAAATTTGATGTATGAACCGTCATTACGACGAACAACTGACTTAGTTCGTACGATTAGAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTACATTTTTaa                                                                                                                                >  1:155916/1‑141 (MQ=255)          tcaCGGATGATAACACATGCATTTTCATCAAATTTGATGTATGAACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACACCGCCACCTGGTGTTGCATTTTTAACa                                                                                                                              <  2:54125/141‑1 (MQ=255)          tcaCGGATGATAACACATGCATTTTCATCAAATTTGATGTATGAACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACa                                                                                                                              <  2:54102/141‑1 (MQ=255)                 tgatAACACATGCATTTTCATCCAATTTGATGTATGAACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACCACGCCACCTTTTTTAACAACGCCCCCGGGTGTTGCATTTTTAACAGTACATa                                                                                                                       >  1:139666/1‑141 (MQ=255)                                          tttGATGTATGAACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGt                                                                                              <  2:145333/141‑1 (MQ=255)                                             gATGTATGAACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGtttt                                                                                           <  2:105850/141‑1 (MQ=255)                                               tGTATGCACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTAc                                                                                         <  2:209778/141‑1 (MQ=255)                                                    gAACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCa                                                                                    <  2:75582/141‑1 (MQ=255)                                                    gAACCGTCAGTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCa                                                                                    <  2:15276/141‑1 (MQ=255)                                                     aaCCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAg                                                                                   <  2:190223/141‑1 (MQ=255)                                                     aaCCGTCATTACGACGAACACCGGACGTAGTACGTACGAATACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAg                                                                                   <  2:12164/141‑1 (MQ=255)                                                              tACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTa                                                                          <  2:181797/141‑1 (MQ=255)                                                                  acgaACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATAc                                                                      <  2:226354/141‑1 (MQ=255)                                                                    gaACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACtt                                                                    <  2:153350/141‑1 (MQ=255)                                                                    gaACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTACATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACtt                                                                    <  2:172751/141‑1 (MQ=255)                                                                    gaACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTACATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACtt                                                                    <  2:155916/141‑1 (MQ=255)                                                                            gACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACTTTGATTGTa                                                            <  1:1340/141‑1 (MQ=255)                                                                             aCTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACTTTGATTGTaa                                                           >  2:87871/1‑141 (MQ=255)                                                                                           aTTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACTTTGATTGTAAGAACTTCACGAGCa                                             >  1:137250/1‑141 (MQ=255)                                                                                             tACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACTTTGATTGTAAGAACTTCACGAGCAcc                                           <  1:194369/141‑1 (MQ=255)                                                                                                       aCAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACTTTGATTGTAAGAACTTCACGAGCACCAGAGTTGTCt                                 <  2:240675/141‑1 (MQ=255)                                                                                                            gTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACTTTGATTGTAAGAACTTCACGAGCACCAGAGTTGTCTGCTAc                            <  2:139666/141‑1 (MQ=255)                                                                                                                ccTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACTTTGATTGTAAGAACTTCACGAGCACCAGAGTTGTCTGCTACTTTc                        <  2:175162/141‑1 (MQ=255)                                                                                                                 cTTTTTTAACACCGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCCCCTAATACTTTGATTGTAAGAACTTCACGAGCACCAGAGTTGTCTGCTACTTTCa                       <  1:79506/141‑1 (MQ=255)                                                                                                                            gcGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACTTTGATTGTAAGAACTTCACGAGCACCAGAGTTGTCTGCTACTTTCAAGCGTGTTTCt            <  1:127690/140‑1 (MQ=255)                                                                                                                            acGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCTCCTAAGACTTTGATTGTAAGAACTTCACGAGCACCAGAGTTGTCTGCTACTTTCAAGCGTGTTTCt            <  1:189259/141‑1 (MQ=255)                                                                                                                              gCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACTTTGATTGTAAGAACTTCACGAGCACCAGAGTTGTCTGCTACTTTCAAGCGTGTTTCttg          <  1:228923/141‑1 (MQ=255)                                                                                                                                     ggTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACTTTGATTGTAAGAACTTCACGAGCACCAGAGTTGTCTGCTACTTTCAAGCGTGTTTCTTGTTGGATc   <  2:124053/141‑1 (MQ=255)                                                                                                                                      gtgtTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACTTTGATTGTAAGAACTTCACGAGCACCAGAGTTGTCTGCTACTTTCAAGCGTGTTTCTTGTTGGATCa  >  1:168298/1‑141 (MQ=255)                                                                                                                                           |                                                                                                                                        CCTTTGTCATCACGGATGATAACACATGCATTTTCATCAAATTTGATGTATGAACCGTCATTACGACGAACACCTGACTTAGTACGTACGATTACAGCTTTGACAACGTCACCTTTTTTAACAACGCCACCTGGTGTTGCATTTTTAACAGTACATACGATAACATCGCCGATGTTTGCTGTTTTACGACCAGATCCACCTAATACTTTGATTGTAAGAACTTCACGAGCACCAGAGTTGTCTGCTACTTTCAAGCGTGTTTCTTGTTGGATCA  >  CP000730/2366081‑2366354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑