Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,201,454	T→C	18.2%	V144A (GTT→GCT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,201,454	0	T	C	18.2%	42.0 / 6.2	22	V144A (GTT→GCT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (10/8);  new base C (2/2);  total (12/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01

GAACAAGCATTAACGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGTCTCCTTCAAGTGAGT  >  CP000730/1201327‑1201594                                                                                                                                |                                                                                                                                             gAACAAGCATGAACGAAAAAAGGTGTTGACCGAAAGAAAATGTCCACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACttt                                                                                                                                 <  1:91429/141‑1 (MQ=255)      aGCATTAACGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGa                                                                                                                            >  1:72685/1‑141 (MQ=255)       gCATTAACGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGAt                                                                                                                           >  1:112507/1‑141 (MQ=255)            aaCGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGCTCGTGGTGACTTTAATGATCCata                                                                                                                      >  2:90539/1‑141 (MQ=255)            aaCGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGCTCGTGGTGACTTTAATGATCCata                                                                                                                      >  2:19351/1‑141 (MQ=255)             aCGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCatat                                                                                                                     <  1:134699/141‑1 (MQ=255)                  aaaaGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTAc                                                                                                                >  2:191689/1‑141 (MQ=255)                                   agaaaATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACg                                                                                               >  1:129745/1‑141 (MQ=255)                                       aaTGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGc                                                                                           >  1:219930/1‑141 (MQ=255)                                        aTGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCt                                                                                          <  1:94136/141‑1 (MQ=255)                                        aTGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCt                                                                                          >  1:47444/1‑141 (MQ=255)                                             aaCAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGcaca                                                                                     >  2:4966/1‑141 (MQ=255)                                                      aTTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTAt                                                                            >  2:31320/1‑141 (MQ=255)                                                                  aTGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAAt                                                                <  2:135887/141‑1 (MQ=255)                                                                  aTGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAAt                                                                <  2:148075/141‑1 (MQ=255)                                                                    gTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGCTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATgg                                                              <  1:19351/141‑1 (MQ=255)                                                                    gTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGCTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATgg                                                              <  1:90539/141‑1 (MQ=255)                                                                         gAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGAt                                                         <  1:189719/141‑1 (MQ=255)                                                                                                   aCAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTaaaaa                               >  1:19078/1‑141 (MQ=255)                                                                                                                  tAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGtc                <  1:163546/141‑1 (MQ=255)                                                                                                                  tAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGtc                <  2:72685/141‑1 (MQ=255)                                                                                                                    gACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGtctc              >  2:89438/1‑141 (MQ=255)                                                                                                                                ttCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGTCTCCTTCAAGTGAgg  >  2:25379/1‑140 (MQ=255)                                                                                                                                ttCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGTCTCCTTCAAGTGAGt  >  2:21192/1‑141 (MQ=255)                                                                                                                                |                                                                                                                                             GAACAAGCATTAACGAAAAAAGGTGTTGACCGAAAGAAAATGTCAACAGCTGAATTCCGTGAGAAATGTAAAGAATTTGCTTTAGAACAAATTGAATTACAGAAAAAAGATTTTAGACGTTTAGGTGTTCGTGGTGACTTTAATGATCCATATATTACATTAAAACCTGAATACGAAGCTGCACAAATTCGTATTTTTGGAGAAATGGCAGATAAAGGTTTAATTTATAAAGGTAAAAAGCCAGTTTATTGGTCTCCTTCAAGTGAGT  >  CP000730/1201327‑1201594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑