| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 748,768 | A→G | 19.5% | I255V (ATT→GTT) | USA300HOU_0694 → | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 748,768 | 0 | A | G | 19.5% | 39.0 / 5.9 | 22 | I255V (ATT→GTT) | USA300HOU_0694 | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (8/9); new base G (2/2); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
AGTGGATCAAGTAGCAACTTGCAAAGAAATGTTGATTAGTGGTGTAGGTGTTACAATCTTGCCAGAAATTATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGCGAGCTTTGTTGAACAACCAAAGGCGTAGTTTTAGACTAATTTAAGGTTTGTATTTAATTTTAAA > CP000730/748632‑748908 | aGTGGATCAAGTAGCAACTTGCAAAGAAATGTTGATTAGTGGTGTAGGTGTTACAATCTTGCCAGAAATTATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGAttcg > 1:198744/1‑141 (MQ=255) aTCAAGTAGCAACTTGCAAAGAAATGTTGATTAGTGGTGTAGGTGTTACAATCTTGCCAGAAATTATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGa < 2:81555/141‑1 (MQ=255) aTCAAGTAGCAACTTGCAAAGAAATGTTGATTAGTGGTGTAGGTGTTACAATCTTGCCAAAAATTATGATGAAAAATATCAGCAAAGAACAAAAAAAAAAAAAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGa < 2:223075/141‑1 (MQ=255) gTAGCAACTTGCAAAGAAATGTTGATTAGTGGTGTAGGTGTTACAATCTTGCCAGAAATTATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGGTTCGTTCGACAttt > 1:164772/1‑141 (MQ=255) gTAGCAACTTGCAAAGAAATGTTGATTAGTGGTGTAGGTGTTACAATCTTGCCAGAAATTATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGGTTCGTTCGACAttt > 1:164735/1‑141 (MQ=255) aaGAAATGTTGATTAGTGGTGTAGGTGTTACAATCTTGCCAGAAATTATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGTGTTAttttt > 2:141128/1‑137 (MQ=255) aaGAAATGTTGATTAGTGGTGTAGGTGTTACAATCTTGCCAGAAATTATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATc > 1:219800/1‑141 (MQ=255) aTGTTGATTAGTGGTGTAGGTGTTACAATCTTGCCAGAAATTATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGc < 1:137555/141‑1 (MQ=255) gTTGATTAGTGGTGTAGGTGTTACAATCTTGCCAGAAATTATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGCAt < 2:230245/141‑1 (MQ=255) aGTGGTGTAGGTGTTACAATCTTGCCAGAAATTATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCaa > 2:245636/1‑141 (MQ=255) tgtAGGTGTTACAATCTTGCCAGAAATTATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGcc < 1:46964/141‑1 (MQ=255) ggTGTTACAATCTTGCCAGAAATTATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACaa > 2:87976/1‑141 (MQ=255) gatgaAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGc > 1:5873/1‑141 (MQ=255) gatgaAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGc < 2:144596/141‑1 (MQ=255) atgaAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGCg < 1:222200/141‑1 (MQ=255) atgaAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGCg < 1:33698/141‑1 (MQ=255) aGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGCGAGCTTTGTTGaa > 2:10311/1‑141 (MQ=255) aaGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGGTTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGCGAGCTTTGTTGaacaac < 2:164735/141‑1 (MQ=255) aaGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGGTTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGCGAGCTTTGTTGaacaac < 2:164772/141‑1 (MQ=255) tAATGAGCCGCTGTTTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGCGAGCTTTGTTGAACAACCAAAGGCGTAGTTTTAGACTAATTTAAGGTTTGt < 2:89070/141‑1 (MQ=255) gAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGCGAGCTTTGTTGAACAACCAAAGGCGTAGTTTTAGACTAATTTAAGGTTTGTAttt < 1:63756/141‑1 (MQ=255) tGATTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGCGAGCTTTGTTGAACAACCAAAGGCGTAGTTTTAGACTAATTTAAGGTTTGTATTTAATTTTa > 1:160243/1‑141 (MQ=255) aTTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGCGAGCTTTGTTGAACAACCAAAGGCGTAGTTTTAGACTAATTTAAGGTTTGTATTTAATTTTaaa > 2:233720/1‑141 (MQ=255) aTTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGCGAGCTTTGTTGAACAACCAAAGGCGTAGTTTTAGACTAATTTAAGGTATGTATTTAATTATaaa > 2:50985/1‑141 (MQ=255) | AGTGGATCAAGTAGCAACTTGCAAAGAAATGTTGATTAGTGGTGTAGGTGTTACAATCTTGCCAGAAATTATGATGAAAAATATCAGCAAAGAACAATTTGAGTTTGAAAAAGTAGAAATTGATAATGAACCGCTGATTCGTTCGACATTTATGAGTTATGATCCGAGCATGTTGCAATTGCCACAAGTTGATTCTTTTGTAAATCTCATGGCGAGCTTTGTTGAACAACCAAAGGCGTAGTTTTAGACTAATTTAAGGTTTGTATTTAATTTTAAA > CP000730/748632‑748908 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |