Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,373,525	G→T	A84E (GCA→GAA)	pbuG ←	NCS2 family nucleobase:cation symporter‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,373,525	0	G	T	94.7%	54.8 / ‑5.7	19	A84E (GCA→GAA)	pbuG	NCS2 family nucleobase:cation symporter‑2
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (10/8);  total (11/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTT  >  CP000730/2373393‑2373659                                                                                                                                     |                                                                                                                                       cTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTTCTAACGCa                                                                                                                                >  1:29494/1‑141 (MQ=255)                  tAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCAGGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTAt                                                                                                               <  1:82516/141‑1 (MQ=255)                  tAATATCGCAAAGAATATACCTGAGAACAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTAt                                                                                                               <  2:15299/141‑1 (MQ=255)                    atatCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTATTa                                                                                                             >  1:1554/1‑141 (MQ=255)                           aaaGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCTAt                                                                                                      >  1:169979/1‑141 (MQ=255)                                atatACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATa                                                                                                 <  2:129175/141‑1 (MQ=255)                                atatACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATa                                                                                                 <  2:25906/141‑1 (MQ=255)                                 tataCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAg                                                                                                <  1:33110/141‑1 (MQ=255)                                                               aaCTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGc                                                                  <  1:182440/141‑1 (MQ=255)                                                                  ttGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTAc                                                               >  2:167551/1‑141 (MQ=255)                                                                                ccATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCtt                                                 >  2:219418/1‑141 (MQ=255)                                                                                          cacaACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAtttt                                       <  1:77500/141‑1 (MQ=255)                                                                                                              aTGCATTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCtt                   >  2:71324/1‑141 (MQ=255)                                                                                                              aTGCATTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCtt                   >  1:6765/1‑141 (MQ=255)                                                                                                                  aTTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAac               >  1:87709/1‑141 (MQ=255)                                                                                                                  aTTTAATCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAac               >  1:16816/1‑141 (MQ=255)                                                                                                                       aTCCCATACCTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTg          <  2:169979/141‑1 (MQ=255)                                                                                                                          ccATACCTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTa       >  2:50977/1‑141 (MQ=255)                                                                                                                               ccTGGTTCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACtt  >  1:147719/1‑141 (MQ=255)                                                                                                                                     |                                                                                                                                       CTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTT  >  CP000730/2373393‑2373659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑