Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,880,716 G→T 9.9% D1501E (GAC→GAA)  fmtB1 ← cell surface anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,880,7160GT 9.9% 157.2 / 4.3 50D1501E (GAC→GAA) fmtB1cell surface anchored protein
Reads supporting (aligned to +/- strand):  ref base G (24/21);  new base T (3/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAATTCATTGATTTTATCTAGTGCTACTTGTCTTTCT  >  CP000730/1880576‑1880854
                                                                                                                                            |                                                                                                                                          
gCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATgg                                                                                                                                            >  1:197752/1‑141 (MQ=255)
  aCGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTc                                                                                                                                          <  2:2737/141‑1 (MQ=255)
         tCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTc                                                                                                                                   >  1:250195/1‑141 (MQ=255)
           attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCgctac                                                                                                                                 >  2:417665/1‑137 (MQ=255)
           attattCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCgctac                                                                                                                                 >  2:302775/1‑137 (MQ=255)
              tttttttAATTGATTTAAAGCAACTTGTTTTTTTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCTCGCTTTTTAGCTTTATATTGTTTCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATTGTCTGGCGTCACTaa                                                                                                                              >  2:307760/7‑141 (MQ=255)
               ttCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTaaa                                                                                                                             <  1:264628/141‑1 (MQ=255)
                   cTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCaa                                                                                                                         <  1:169884/141‑1 (MQ=255)
                   cTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCaa                                                                                                                         <  1:137927/141‑1 (MQ=255)
                         gATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCGCTACTAAAGCAATGCTAt                                                                                                                   <  1:341344/141‑1 (MQ=255)
                         gATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCGCTACTAAAGCAATGCTAt                                                                                                                   <  2:364864/141‑1 (MQ=255)
                           tttAAAGCAACATGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCa                                                                                                                 <  2:278078/141‑1 (MQ=255)
                               aaGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAgcg                                                                                                             <  1:48522/141‑1 (MQ=255)
                                    aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                                                                                        >  2:363787/1‑141 (MQ=255)
                                    aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                                                                                        >  1:152818/1‑141 (MQ=255)
                                    aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                                                                                        >  2:397000/1‑141 (MQ=255)
                                    aCTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGa                                                                                                        >  1:418040/1‑141 (MQ=255)
                                     cTTGTTTTTCTTCATCAGTCGCATGTTCAGCTTGCTCTATTTCTTGCTTTTTAGCCTCATATTGTTGCTTTACTGCATCTCTAGCAGCTGCTCTAACAATATGTTCAGGCGCTACTAAAGCAATGCTATCAAGCGCTTGAc                                                                                                       >  1:429216/1‑141 (MQ=255)
                                       tGTTTTTCTTCATCAGGCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACtt                                                                                                     >  2:204606/1‑141 (MQ=255)
                                                          cATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgtt                                                                                  <  2:362767/141‑1 (MQ=255)
                                                          cATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgtt                                                                                  <  1:399199/141‑1 (MQ=255)
                                                          cATGTTCCGCTCGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgtt                                                                                  <  1:365245/141‑1 (MQ=255)
                                                           aTGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACttgttg                                                                                 <  1:339573/141‑1 (MQ=255)
                                                            tGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACACTATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGa                                                                                >  1:335211/1‑141 (MQ=255)
                                                                  gCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGtt                                                                          <  2:328526/141‑1 (MQ=255)
                                                                      gCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtat                                                                      >  1:391697/1‑141 (MQ=255)
                                                                         cAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtattat                                                                   <  1:162647/141‑1 (MQ=255)
                                                                         cAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCtattat                                                                   >  1:26570/1‑141 (MQ=255)
                                                                           aTTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTc                                                                 <  2:26701/141‑1 (MQ=255)
                                                                                        tAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCa                                                    <  1:278911/141‑1 (MQ=255)
                                                                                         aGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCAt                                                   >  2:170061/1‑141 (MQ=255)
                                                                                          gCTTCATATTGTTGCTTAACTGAATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATg                                                  >  2:152908/1‑141 (MQ=255)
                                                                                           cTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATgg                                                 >  2:91275/1‑141 (MQ=255)
                                                                                            ttCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGc                                                >  1:291294/1‑141 (MQ=255)
                                                                                                     ttgCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTAc                                       >  1:101368/1‑141 (MQ=255)
                                                                                                     ttgCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTAc                                       <  1:11291/141‑1 (MQ=255)
                                                                                                         ttAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAAt                                   >  1:127858/1‑141 (MQ=255)
                                                                                                         ttAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAAt                                   <  2:127879/141‑1 (MQ=255)
                                                                                                             cTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGGTTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAATTCAt                               <  1:363787/141‑1 (MQ=255)
                                                                                                                cATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAATTCATTGa                            >  1:439502/1‑141 (MQ=255)
                                                                                                                 aTCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAATTCATTGAt                           >  1:322140/1‑141 (MQ=255)
                                                                                                                 aTCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAATTCATTGAt                           >  1:322132/1‑141 (MQ=255)
                                                                                                                  tctcTAGCAGCTGCTATAACAATATGGTTAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAATTCATTGAtt                          >  2:342248/1‑141 (MQ=255)
                                                                                                                      tAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGCTTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAATTTTATTGATTTTAt                      >  2:115854/1‑141 (MQ=255)
                                                                                                                      tAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGGTGTATCATCAACTTGTTGATGTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAATTCATTGATTTTAt                      >  1:97340/1‑141 (MQ=255)
                                                                                                                       agcagcTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTTATTTACAAATTCATTGATTTTATc                     <  2:267860/141‑1 (MQ=255)
                                                                                                                          agcTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAATTCATTGATTTTATCTAg                  >  2:420966/1‑141 (MQ=255)
                                                                                                                                 ctAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAATTCATTGATTTTATCTAGTGCTACt           >  2:273771/1‑141 (MQ=255)
                                                                                                                                       atatGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAATTCATTGATTTTATCTAGTGCTACTTGtctt     <  1:388914/141‑1 (MQ=255)
                                                                                                                                       atatGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGCCATGGCTTGATTTACAAATTCATTGATTTTATCTAGTGCTACTTGtctt     <  1:221913/141‑1 (MQ=255)
                                                                                                                                          tGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAATTCATTGATTTTATCTAGTGCTACTTGtctttct  <  2:300532/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
GCACGTTTTTCATTATTCGCTAATTGATTTAAAGCAACTTGTTTTTCTTCATCAGTCGCATGTTCCGCTTGCTCAATTTCGCGCTTTTTAGCTTCATATTGTTGCTTAACTGCATCTCTAGCAGCTGCTCTAACAATATGGTCAGGCGTCACTAAAGCAATGCTATCAAGCGCTTGACTTGTTGTATCATCAACTTGTTGATTTGTTCTATTATTCGTAATATCTGTCATGGCTTGATTTACAAATTCATTGATTTTATCTAGTGCTACTTGTCTTTCT  >  CP000730/1880576‑1880854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑