| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,034,893 | G→A | 12.2% | intergenic (‑1310/‑6396) | USA300HOU_0983 ← / → USA300HOU_0984 | competence protein/glycosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,034,893 | 0 | G | A | 12.2% | 104.8 / 5.2 | 41 | intergenic (‑1310/‑6396) | USA300HOU_0983/USA300HOU_0984 | competence protein/glycosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (15/21); new base A (3/2); total (18/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
ACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCATTGCTAA > CP000730/1034759‑1035033 | aCAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCa > 2:301007/1‑141 (MQ=255) ttCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGatata < 2:76114/141‑1 (MQ=255) tttttGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGAtt > 2:125254/1‑141 (MQ=255) tttttGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACAACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGAtt < 1:436399/141‑1 (MQ=255) tttGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTa < 1:325503/141‑1 (MQ=255) tGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACt < 2:320195/141‑1 (MQ=255) aTTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCctc < 2:322342/141‑1 (MQ=255) gAAACTGTTACCATCATCATCTGTTATACAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGt < 1:20225/141‑1 (MQ=255) gAAACTGTTACCATCATCATCTGTTATAATACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGt < 1:20223/141‑1 (MQ=255) gAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGt < 2:45042/141‑1 (MQ=255) aCTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAGTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTAtt > 1:99375/1‑141 (MQ=255) tGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTAACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTAtttt < 1:128717/141‑1 (MQ=255) gTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATg > 1:240211/1‑141 (MQ=255) aaaCTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGAt < 1:429/141‑1 (MQ=255) aaGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTa > 1:121171/1‑141 (MQ=255) aaaTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATg < 1:190337/141‑1 (MQ=255) aaTTGCCAGTATCATGATAAACAGCGCCACTACCAGAAATTCTTCTTACTACTTCTATGTTGTGAGCATCGATATATGTCTGATTTACTTTCTCTATCGTATTTTGATTCTTTCCAACTATGATAGATAGTCTATTTATGt < 2:274331/141‑1 (MQ=255) aaTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGt > 1:323754/1‑141 (MQ=255) gCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATGTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGtaaaa < 2:31810/141‑1 (MQ=255) cATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGt < 2:408751/141‑1 (MQ=255) cATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGt < 2:26581/141‑1 (MQ=255) aTAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGATGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACt < 1:64665/141‑1 (MQ=255) aaaCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt > 2:80812/1‑141 (MQ=255) aaaCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt > 2:80803/1‑141 (MQ=255) aaaCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt < 2:438448/141‑1 (MQ=255) aaaCAGCGCCACCAACAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt < 2:438446/141‑1 (MQ=255) cAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTttctt < 1:290547/141‑1 (MQ=255) gcgcCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTtcttct > 1:61616/1‑141 (MQ=255) caccaccaGAAATGCTTCTAACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATACAAAGTAACTTTCTTctgctg > 2:455876/1‑141 (MQ=255) caccaccaGAAATGCTTCTAACTACATCGATGTTATGAACATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAGAAAGTAACTTTCTTctgctt > 2:455982/1‑140 (MQ=255) accaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTgg < 1:231155/141‑1 (MQ=255) ccaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGt > 1:447527/1‑141 (MQ=255) caGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAt > 1:87225/1‑141 (MQ=255) aaaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtttt > 2:133966/1‑141 (MQ=255) aaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTAGTAAAttttt < 1:202757/141‑1 (MQ=255) tcttctTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTaa > 1:191896/1‑141 (MQ=255) cttcttACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTaaa > 2:221275/1‑141 (MQ=255) cttctaACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCca > 1:104032/1‑75 (MQ=255) cttctaACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCca < 2:104032/75‑1 (MQ=255) gATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCAt > 1:194190/1‑141 (MQ=255) aTGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCAtt < 1:64679/141‑1 (MQ=255) ttgtGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCATTGCt < 1:417770/141‑1 (MQ=255) gtgAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCATTGCTaa > 2:258541/1‑141 (MQ=255) | ACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCATTGCTAA > CP000730/1034759‑1035033 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |