Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 987,475 A→T 11.6% intergenic (+48/+7) fabH2 → / ← USA300HOU_0943 3‑oxoacyl‑[acyl‑carrier‑protein] synthase/hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730987,4750AT11.6% 87.3 / 2.8 32intergenic (+48/+7)fabH2/USA300HOU_09433‑oxoacyl‑[acyl‑carrier‑protein] synthase/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (13/15);  new base T (2/2);  total (15/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGACCTTGATATTACTTATGCAATGAGTAATAGCTTAGGATTCGGTGGACATAACGCAGTATTAGTATTCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATGTAGAAACAAAGTAATGCTCTTTTTTTACCTCTTGTGGGTTGAAAAATGGATCATCA  >  CP000730/987338‑987614
                                                                                                                                         |                                                                                                                                           
cAAGACCTTGATATTACTTATGCAATGAGTAATAGCTTAGGATTCGATGGACATAACGCAGTATTAGTATTCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGtt                                                                                                                                          >  1:379582/1‑141 (MQ=255)
  agcgcaagtttttACTTATGACATGAGTAATAGCTTAGGATTCGGTGGAGATAACGCAGTATTTGTATTCAAGAAATTTGAAGCATAATTATAAAGATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTc                                                                                                                                        <  2:308637/130‑1 (MQ=255)
     ccTTGATATTACTTATGCAATGAGTAATAGCTTAGGATTCGGTGGACATAACGCAGTATTAGTATTCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCttt                                                                                                                                     <  1:341998/141‑1 (MQ=255)
        tGATATTACTTATGCAATGAGTAATAGCTTAGGATTCGGTGGACATAACGCAGTATTAGTATTCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTtata                                                                                                                                  >  1:56789/1‑141 (MQ=255)
         gATATTACTTATGCAACGAATAATAGCTTAGGATTCGGAGGACATAACGCAGTATTAGTATTAAAGAAATTTGAAGCATAACTATAAAAAATTTCAGTCACGTTGTTTTGGTTACTGAAGATGTTTTTAGGTTCTCtattt                                                                                                                                 >  2:92244/1‑139 (MQ=255)
               aCTTATGCAATGAGTAATAGCTTAGGATTCGGTGGACATAACGCAGTATTAGTATTCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGAt                                                                                                                           >  2:99483/1‑141 (MQ=255)
                         tGAGTAATAGCTTAGGATTCGGTGGACATAACGCAGTATTAGTATTCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGc                                                                                                                 <  1:32534/141‑1 (MQ=255)
                                 aGCTTAGGATTCGGTGGACATAACGCAGTATTAGTATTCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGt                                                                                                         <  1:247377/141‑1 (MQ=255)
                                      aGGATTCGGTGGACATAACGCAGTATTAGTATTCAAGAAATTTGAAGCATAATTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCTGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCataa                                                                                                    <  2:175352/141‑1 (MQ=255)
                                         aTTCGGTGGACATAACGCAGTATTAGTATTCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCAtaataa                                                                                                 >  2:300842/1‑141 (MQ=255)
                                                     tAACGCAGTATTAGTATTCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATaaaatataaaatat                                                                                     <  1:92244/141‑1 (MQ=255)
                                                      aaCGCAGTATTAGTATTCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAAtatt                                                                                    <  1:276560/141‑1 (MQ=255)
                                                      aaCGCAGTATAAGTATTCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAAtatt                                                                                    >  2:11774/1‑141 (MQ=255)
                                                           agtattagtattCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATttatta                                                                               <  2:356647/141‑1 (MQ=255)
                                                               ttagtattCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATtaataa                                                                           >  1:193387/1‑141 (MQ=255)
                                                                       cAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGa                                                                   <  1:11774/141‑1 (MQ=255)
                                                                                tGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATg                                                          >  2:37697/1‑141 (MQ=255)
                                                                                tGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATg                                                          >  2:85190/1‑141 (MQ=255)
                                                                                 gAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAGGGGGATTATCCATGt                                                         <  2:326146/141‑1 (MQ=255)
                                                                                 gAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATGt                                                         <  1:196410/141‑1 (MQ=255)
                                                                                  aaGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATGTa                                                        >  2:358134/1‑141 (MQ=255)
                                                                                   aGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATGTAg                                                       <  2:102272/141‑1 (MQ=255)
                                                                                    gCATAATTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCTGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAGAATATTTATTAATAATAAGGGGATTATCCATGTAGa                                                      >  2:393844/1‑141 (MQ=255)
                                                                                             taAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATGTAGAAACAAAGTa                                             >  1:216252/1‑141 (MQ=255)
                                                                                                        aGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATGTAGAAACAAAGTAATGCTCttttt                                  <  2:373342/141‑1 (MQ=255)
                                                                                                             ctaaaacaacgTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATGTAGAAACAAAGTAATGCTCTTTTTTTAcc                             >  1:113156/11‑141 (MQ=255)
                                                                                                             cGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATGTAGAAACAAAGTAATGCTCTTTTTTTAcc                             <  2:379582/141‑1 (MQ=255)
                                                                                                               ttgttTTAGTTACTGAAGATTTTTTTTTTTTTTTTATACTAAGATGAGCGACAGCACAATTGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATGTAGAAACAAAGTAATGCTCTTTTTTTATctt                           >  2:323951/1‑140 (MQ=255)
                                                                                                                         tACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATGTAGAAACAAAGTAATGCTCTTTTTTTACCTCTTGTGGGTTg                 <  1:12095/141‑1 (MQ=255)
                                                                                                                                 aTTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATGTAGAAACAAAGTAATGCTCTTTTTTTACCTCTTGTTGGTTGAAAAATgg         >  2:20151/1‑141 (MQ=255)
                                                                                                                                  ttttttCTGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAGAATATTTATTAATAATAAGGGGATTATCCATGTAGAAACAAAGTAATGCTCTTTTTTTACCTCTTGTGGGTTGAAAAATGGa        <  1:393844/141‑1 (MQ=255)
                                                                                                                                        cAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATGTAGAAACAAAGTAATGCTCTTTTTTTACCTCTTGTGGGTTGAAAAATGGAtcatca  <  1:358134/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
CAAGACCTTGATATTACTTATGCAATGAGTAATAGCTTAGGATTCGGTGGACATAACGCAGTATTAGTATTCAAGAAATTTGAAGCATAACTATAAAAATCTTCAGTAACGTTGTTTTAGTTACTGAAGATTTTTTCAGTTTCTTTATACTAAGATGAGCGACAGCACAATCGTCATAATAAAATATAAAATATTTATTAATAATAAGGGGATTATCCATGTAGAAACAAAGTAATGCTCTTTTTTTACCTCTTGTGGGTTGAAAAATGGATCATCA  >  CP000730/987338‑987614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑