Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 770,855 A→G 13.5% intergenic (+41/‑212) ebsC → / → fruR possible transcriptional regulator/DeoR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730770,8550AG13.5% 81.2 / 7.0 37intergenic (+41/‑212)ebsC/fruRpossible transcriptional regulator/DeoR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (19/13);  new base G (2/3);  total (21/16)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

TGGGCTTCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTT  >  CP000730/770727‑770991
                                                                                                                                |                                                                                                                                        
tggGCTTCGAACAATGCAAATCTCAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAAt                                                                                                                              >  2:319694/1‑141 (MQ=255)
 gggCTTCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAAtt                                                                                                                             >  1:154106/1‑141 (MQ=255)
              tGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCa                                                                                                                >  1:436850/1‑141 (MQ=255)
                 aaaTCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTAAtct                                                                                                             >  2:452011/1‑139 (MQ=255)
                       caATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAAATGAATTTTATAAAAATTTCATCTCTGTca                                                                                                       <  2:448427/141‑1 (MQ=37)
                         aTAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCcaca                                                                                                     >  2:14959/1‑141 (MQ=255)
                           aGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAAATGAATTTTATAAAAATTTCATCTCTGTCACACt                                                                                                   <  1:247372/141‑1 (MQ=25)
                              tGTTGAGGATTTGATTACAATACCTAAAGGCATAATTGGAGCAGTTATTCATGAATGATTAATACCTACAAAAAGTATGGGGCAAAATTAGGGGTGTGACGTAGATGAAATTTTTATAAAATTCATTTCTGCCACTCTCCt                                                                                                <  2:4047/141‑1 (MQ=255)
                                   aGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTttg                                                                                           >  1:258730/1‑141 (MQ=255)
                                         tGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGaa                                                                                     >  1:127292/1‑141 (MQ=255)
                                                   aaCTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCAtttt                                                                           >  2:221989/1‑141 (MQ=255)
                                                         aGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATc                                                                     >  1:25364/1‑141 (MQ=255)
                                                          ggCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCa                                                                    <  1:297460/141‑1 (MQ=255)
                                                              aaaTTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATaaa                                                                >  1:309633/1‑141 (MQ=255)
                                                                 ttGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAg                                                             <  1:180752/141‑1 (MQ=255)
                                                                  tGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGt                                                            <  1:107083/141‑1 (MQ=255)
                                                                  tGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGAAAGATTAGGAGTGTGGCAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGt                                                            <  2:114826/141‑1 (MQ=255)
                                                                    gAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCa                                                          >  2:417402/1‑141 (MQ=255)
                                                                    gAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCa                                                          >  2:13268/1‑141 (MQ=255)
                                                                      gCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCAtt                                                        >  1:232201/1‑141 (MQ=255)
                                                                             tCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTg                                                 >  1:138371/1‑141 (MQ=255)
                                                                                attaataatTAATAAAAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCAGAAACAGTCATTATAATTGAGt                                              <  2:23277/138‑1 (MQ=255)
                                                                                 tgaatgaTTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTa                                             >  1:51598/1‑141 (MQ=255)
                                                                                      gaTTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGa                                        >  1:303324/1‑141 (MQ=255)
                                                                                          aataaCAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACAt                                    >  2:309984/1‑141 (MQ=255)
                                                                                                  caaAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAAAATAAAAATGt                            <  2:2461/141‑1 (MQ=255)
                                                                                                   aaaaaGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTa                           >  1:69001/1‑141 (MQ=255)
                                                                                                      aagaaaggggCAAGCTTAGGAGTGTGACAGAGACCAACATTTCATAAAACTCATATCCGCCACACTCCTTATAGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAATATTAGAAAAAAACAATGTAAta                        >  2:153322/7‑140 (MQ=255)
                                                                                                       aGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAAttt                       >  2:133190/1‑141 (MQ=255)
                                                                                                              ggCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAGAAATTAAGTTTATCGTc                <  1:104884/141‑1 (MQ=255)
                                                                                                              ggCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTc                <  1:242340/141‑1 (MQ=255)
                                                                                                              ggCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTc                <  2:76814/141‑1 (MQ=255)
                                                                                                              ggCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTACTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTc                <  2:130858/141‑1 (MQ=255)
                                                                                                                cAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTcac              <  2:56919/141‑1 (MQ=255)
                                                                                                                       aGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGa       <  2:122794/141‑1 (MQ=255)
                                                                                                                        ggAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAg      >  1:368982/1‑141 (MQ=255)
                                                                                                                            tgtgACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGtt  <  1:150780/141‑1 (MQ=255)
                                                                                                                                |                                                                                                                                        
TGGGCTTCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTT  >  CP000730/770727‑770991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: