Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	725,201	A→C	9.1%	I30L (ATT→CTT)	dhaK →	glycerone kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	725,201	0	A	C	9.1%	109.1 / 5.5	44	I30L (ATT→CTT)	dhaK	glycerone kinase
Reads supporting (aligned to +/- strand):  ref base A (20/20);  new base C (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

CATTCATTTATTCTGTTTTATACTATGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGCCGGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGC  >  CP000730/725062‑725335                                                                                                                                            |                                                                                                                                       cattcattTATTCTGTTTTATACTATGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGAt                                                                                                                                       <  1:217938/141‑1 (MQ=255)  attcattTATTCTGTTTTATACTATGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGAtt                                                                                                                                      <  1:150760/141‑1 (MQ=255)      attTATTCTGTTTTATACTATGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACTCAGAGTTAGATCTGATTGCTa                                                                                                                                  <  1:140827/141‑1 (MQ=255)       tttATTCTGTTTTATACTATGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTaa                                                                                                                                 <  1:119393/141‑1 (MQ=255)        ttATTCTGTTTTATACTATGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAAt                                                                                                                                <  1:312960/141‑1 (MQ=255)        ttATTCTATTTTATACTATAACTGATAATACCAAGGAGGTACAAGATGATGAAAAAGTTTATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAAt                                                                                                                                <  2:348279/141‑1 (MQ=255)         tATTCTGTTTTATACTATGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATa                                                                                                                               >  2:365722/1‑141 (MQ=255)         tATTCTGTTTTATACTATGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATa                                                                                                                               >  2:231146/1‑141 (MQ=255)          aTTCTGTTTTATACTATGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGCTTGCTAATAc                                                                                                                              >  1:359167/1‑141 (MQ=255)          aTTCTGTTTTATACTATGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGCTTGCTAATAc                                                                                                                              >  1:235872/1‑141 (MQ=255)               gTTTTATACTATGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTa                                                                                                                         >  1:429545/1‑141 (MQ=255)                  ttATACTATGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACaaataaag                                                                                                                      <  2:7054/141‑8 (MQ=255)                         aTGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTaaaaaa                                                                                                               >  1:61066/1‑141 (MQ=255)                             cTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCt                                                                                                           <  1:80984/141‑1 (MQ=255)                                     aCCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTaagaaaga                                                                                                   <  2:379176/141‑1 (MQ=255)                                        aaGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACa                                                                                                <  2:221651/141‑1 (MQ=255)                                              ggacaataTGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGgtgt                                                                                          <  2:293009/139‑1 (MQ=255)                                               tACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTa                                                                                         >  1:384531/1‑141 (MQ=255)                                                 caacaTGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCATGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGc                                                                                       >  2:127720/1‑141 (MQ=255)                                                    caTGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAAt                                                                                    >  1:97332/1‑141 (MQ=255)                                                      tgatgaAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGAAAAAAAAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAg                                                                                  <  2:199233/141‑1 (MQ=255)                                                        atgaAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGtc                                                                                <  1:40525/141‑1 (MQ=255)                                                        atgaAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGtc                                                                                >  2:81925/1‑141 (MQ=255)                                                          gaAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGCTTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGtctc                                                                              <  2:359167/141‑1 (MQ=255)                                                          gaAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGCTTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGtctc                                                                              <  2:235872/141‑1 (MQ=255)                                                                    aTCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGa                                                                    <  2:38628/141‑1 (MQ=255)                                                                            aaaaGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACa                                                            >  1:345488/1‑141 (MQ=255)                                                                                gAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGaa                                                        >  1:261083/1‑141 (MQ=255)                                                                                gAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGaa                                                        >  2:368188/1‑141 (MQ=255)                                                                                gAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGaa                                                        >  1:156812/1‑141 (MQ=255)                                                                                     aTTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTgc                                                   >  1:74448/1‑141 (MQ=255)                                                                                     aTTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCCGGAGGTGGAAGCGGACATGAACCTgc                                                   >  1:397773/1‑141 (MQ=255)                                                                                      tttttAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTgcg                                                  <  1:231146/141‑1 (MQ=255)                                                                                        tttAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCa                                                <  1:205084/141‑1 (MQ=255)                                                                                           aaCTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGc                                             >  1:228126/1‑141 (MQ=255)                                                                                            aCTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGcc                                            <  1:230348/141‑1 (MQ=255)                                                                                             cTGATATGCTTGAAGGATTGTTAATTGCGCACCCAAAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGCCg                                           <  2:87518/141‑1 (MQ=255)                                                                                              tGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGCCgg                                          >  1:71744/1‑141 (MQ=255)                                                                                                       tGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGCCGGTTTTGTTGc                                 >  1:104603/1‑141 (MQ=255)                                                                                                        gAAGGATTGTTAAATCCGCACCAAGAGTTATAGCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGCAGGTTTTGTTGCa                                >  2:342413/1‑141 (MQ=255)                                                                                                               tGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGCCGGTTTTGTTGCAGAAGGTa                         >  1:156229/1‑141 (MQ=255)                                                                                                                  taagcgcgcTCCCCGAGCAAGATCACATTGCTAATACAGTTAGTATAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGGGGAAGCGGACAGGAACCTGCGCATGCCGGTTTTGTTGCAGAAGGTATGc                      <  1:126895/136‑1 (MQ=255)                                                                                                                                gagTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGCCGGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGtt        <  1:381934/141‑1 (MQ=255)                                                                                                                                      gATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGCCGGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGc  >  1:28993/1‑141 (MQ=255)                                                                                                                                            |                                                                                                                                       CATTCATTTATTCTGTTTTATACTATGACTGATAATACCAAGGAGGTACAACATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGCCGGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGC  >  CP000730/725062‑725335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑