| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 401,596 | A→G | 10.3% | K47K (AAA→AAG) | USA300HOU_0370 → | possible transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 401,596 | 0 | A | G | 10.3% | 116.6 / 3.2 | 47 | K47K (AAA→AAG) | USA300HOU_0370 | possible transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (19/23); new base G (3/2); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGTAATCGATTGAAAGAATTACGAGCACGAGATGGCTTAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTACTGGGTGGACTTGTAGGGGGTATCATAGGTGGAATTTTAGGTAATTT > CP000730/401457‑401735 | tgCGTAATCGATTGAAAGAATTACGAGCACGAGATGGCTTAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATaaaaa > 2:171917/1‑141 (MQ=255) gCGTAATCGATTGAAAGAATTACGAGCACGAGATGGCTTAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAAt > 2:167699/1‑141 (MQ=255) gCGTAATCGATTGAAAGAATTACGAGCACGAGATGGCTTAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAAt > 2:408292/1‑141 (MQ=255) aaTCGATTGAAAGAATTACGAGCACGAGATGGCTTAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACACTTTTATGCCATCAGTATTAACGGCAATAAAAATTGCt > 2:260481/1‑141 (MQ=255) tCGATTGAAAGAATTACGAGCACGAGATGGCTTAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCg < 2:60011/141‑1 (MQ=255) gttACGAGCACGAGATGGCTTAAATCAAACGCAGCTTGCCAAACTAGCTGGTGTTTCAAGACAAACCATTTCGCTAATTGAGCGAAACAATTTTATGCCATCACTATTAACGGCAATAAAGATTGCTCGCATTTTCAATGa > 2:413761/2‑141 (MQ=255) cGAGCACGAGATGGCTTAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACg > 1:223408/1‑141 (MQ=255) cGAGCACGAGATGGCTTAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACg > 1:54759/1‑141 (MQ=255) cACGAGATGGCTTAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGTCAATAAAAATTGCTCGCATTTTCAATGAAACGGTgg > 2:308343/1‑141 (MQ=255) agaTGGCTTAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAAc > 2:203681/1‑141 (MQ=255) aTGGCTTAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTg < 1:374419/141‑1 (MQ=255) tAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTtta < 1:279326/141‑1 (MQ=255) tAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTAGAAACTGTTTtta > 2:62302/1‑141 (MQ=255) aaaCCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTttat < 2:103806/141‑1 (MQ=255) atcAAACGCAGCTTGCCAAACTAGCTGGTGTTTCAAGACAAACCATTTCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAGATTGCTCGCATTTTCAATGAAACGGTGGAAACGGTTTttatta < 1:39702/139‑1 (MQ=255) ccAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTtattat < 1:103861/141‑1 (MQ=255) aaCGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTga < 2:261695/141‑1 (MQ=255) cGCAACTTGCTACACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTgagg < 2:366581/141‑1 (MQ=255) aCTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAggagga > 1:188210/1‑141 (MQ=255) gCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTTATCGATACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAACCGGGGGAAACTGTTTTTATTATTGAGGAGGATGAg > 2:386808/1‑141 (MQ=255) gCTAAACAAGCGGGCGTTCCAAGACAAACCATATCGCTAATTGATCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAg > 2:134054/1‑141 (MQ=255) gCTAAACAAGCGGGCGTTACAAGACAAACCATATCGCTACTTGAGCGAAACAATTTTATGCCATCGGTATTTACGACAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAg < 1:386641/141‑1 (MQ=255) aaCATGCGGGCCTACAAAAACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAGCGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCAt < 2:116309/141‑1 (MQ=255) cAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGa < 2:59261/141‑1 (MQ=255) cGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAAt > 2:246349/1‑141 (MQ=255) gggCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATa > 2:106301/1‑141 (MQ=255) tttCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGAATGAGGCATGAAAATACTAAg > 1:241879/1‑141 (MQ=255) aCAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATc > 1:306128/1‑141 (MQ=255) aaCCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGa < 1:292928/141‑1 (MQ=255) ccATATCGCTAATTGAGCGCAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAACCTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGata < 1:7697/141‑1 (MQ=255) atatCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATc < 2:185775/141‑1 (MQ=255) tatCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCt < 1:188822/141‑1 (MQ=255) atCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGatatccgatatatctt < 1:356409/141‑13 (MQ=255) atCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCtt < 1:167919/141‑1 (MQ=255) gctcaattaGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTa > 2:286487/9‑141 (MQ=255) gCTAATTGAGAGAAACAATTTTATGCCATCAGTATAAAAAAAAAAAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTa < 2:260655/141‑1 (MQ=255) cTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTAc < 1:167699/141‑1 (MQ=255) cTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTAc < 1:408292/141‑1 (MQ=255) cGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTACTGGGTGGAc < 2:371862/141‑1 (MQ=255) aCAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTACTGGGCGGACTTGt > 1:61711/1‑141 (MQ=255) ttATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTACTGGGTGGACTTGTAggggg > 2:80985/1‑141 (MQ=255) tATGCCATCAGTATTAACGGCAATAAAGATTGCTCGCATTTTCAATGAAACGGTGGAAACGGTTTTTATTATTGAGGAGGATGAAGCATGAAAATACTAAGATATATCGGATATCTTTTACTGGGTGGAATTGTAGGGGGt > 2:337179/1‑141 (MQ=255) aGTATTAACGGCAATAAAGATTGCTCGCATTTTCAATGAAACGGTGGAAACGGTTTTTATTATTGAGGAGGATGAAGCATGAAAATACTAAGATATATCGGATATCTTTTACTGGGTGGAATTGTAGGGGGTATCATTGGt < 1:419243/141‑1 (MQ=255) aGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTACTGGGTGGACTTGTAGGGGGTATCATAGGt < 1:25801/141‑1 (MQ=255) aGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTACTGGGTGGACTTGTAGGGGGTATCATAGGt < 1:25278/141‑1 (MQ=255) tATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTACTGGGTGGACTTGTAGGGGGTATCATAGGTgg < 2:256725/141‑1 (MQ=255) aaCGGCAATAAAGATTGCTCGCATTTTCAATGAAACGGTGGAAACGGTTTTTATTATTGAGGAGGATGAAGCATGAAAATACTAAGATATATCGGATATCTTTTACTGGGTGGAATTGTAGGGGGTATCATTGGTGGAAtt > 1:132/1‑141 (MQ=255) aTAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTACTGGGTGGACTTGTAGGGGGTATCATAGGTGGAATTTTAGGTa > 1:438089/1‑141 (MQ=255) aaaTTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTACTGGGTGGACTTGTAGGGGGTATCATAGGTGGAATTTTAGGTAAttt > 1:318580/1‑141 (MQ=255) | TGCGTAATCGATTGAAAGAATTACGAGCACGAGATGGCTTAAACCAAACGCAACTTGCTAAACAAGCGGGCGTTTCAAGACAAACCATATCGCTAATTGAGCGAAACAATTTTATGCCATCAGTATTAACGGCAATAAAAATTGCTCGCATTTTCAATGAAACGGTGGAAACTGTTTTTATTATTGAGGAGGATGAGGCATGAAAATACTAAGATATATCGGATATCTTTTACTGGGTGGACTTGTAGGGGGTATCATAGGTGGAATTTTAGGTAATTT > CP000730/401457‑401735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |